August 2019 | Volume 32 Number 8 www.chromatographyonline.com LC TROUBLESHOOTING Ion-exchange separations and MS PERSPECTIVES IN MODERN HPLC HPLC pumps ANALYSIS FOCUS SEC in polymer analysis Does your carrier gas come with hidden dangers? A Clear Vision A novel automated GC method to detect organochlorine pesticides in environmental waters magentablackcyanyellow ES107242_LCE0819_CVTP1_FP.pgs 08.02.2019 16:57 UBM BIP® Technology Changing the way you think about high purity gas The BIP® range of gases are available with a minimum purity grade of BIP®BIP® gases gases - Performance - Performance with with GC-FID GC-FID 6.0, however is purity everything? Even with a purity level of 99.9999% 12001200 what exactly is in the other 0.0001%? Despite being an extremely tiny 11801180 percentage, what if some “active” impurities were still affecting your 11601160 results and processes? Air Products’ ultra-high purity gases delivered 11401140 with BIP® technology guarantee the market’s lowest possible levels of 11201120 potentially harmful impurities: oxygen (≤ 10 ppb), moisture (≤ 20 ppb) 11001100 and total hydrocarbons (< 100 ppb). 10601060 10601060 BIP® Technology 2 2 4 4 6 6 8 8 1010 12 12 14 14 16 16 18 18min. min. FID baselineFID baseline with with BIP® BIP® N2 as N markup2 as markup gas gas The BIP® technology hidden in every cylinder uniquely filters out FID baselineFID baseline with with non-BIP® non-BIP® N2 as N markup2 as markup gas gas potentially damaging impurities before the gas leaves the cylinder. BIP® technology is available with the most commonly used ultra-high purity gases: Helium, Nitrogen, Hydrogen and Argon. Refill bypass vlave “Switching over to BIP® cylinder technology is very easy because no adaptation of the current system is required.” Dr Frank David, R&D Manager, Research Institute for Purifier Chromatography, Belgium. bed 0800 389 0202 [email protected] airproducts.co.uk/BIP magentablackcyanyellow ES107241_LCE0819_CVTP2_FP.pgs 08.02.2019 16:57 UBM August 2019 | Volume 32 Number 8 www.chromatographyonline.com LC TROUBLESHOOTING Ion-exchange separations and MS PERSPECTIVES IN MODERN HPLC HPLC pumps ANALYSIS FOCUS SEC in polymer analysis A Clear Vision A novel automated GC method to detect organochlorine pesticides in environmental waters Supelco® spectrophotometry products. Supreme performance. Every time. Maintaining your high standards requires analytical products you can rely on, along with the right documentation and expertise. That's why our system of cell and reagent test kits, certifi ed reference materials, sample preparation instruments, spectrophotometers, software and accessories are thoughtfully composed to ensure you reliably achieve your analytical goals and comply with guidelines and regulations. So for pitch-perfect performance day after day, choose Supelco® spectrophotometry products. For more information, please visit: SigmaAldrich.com/SuccessReplicated Merck, the vibrant M and Supelco are trademarks of Merck KGaA, Darmstadt, Germany or its affi liates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources. © 2019 Merck KGaA, Darmstadt, Germany and/or its affi liates. All Rights Reserved. The Life Science Business of Merck operates as MilliporeSigma in the US and Canada. Coupling powers Pioneering new fields in ultra-trace analysis, the Comfortable, easy change of accessories new GCMS-TQ 8050 NX triple quadrupole couples through the advanced, illuminated GC oven the powers of a world-leading GC and a newly designed detector. Both provide outstanding sensi- The GCMS-TQ 8050 NX complements the Shimadzu tivity at femtogram and even sub-femtogram levels. NX family, coupling the Nexis GC-2030 with the quadrupole series TQ-8050, TQ-8040 or QP-2020. Superior performance of NX technologies Shimadzu’s NX series provides high-end GCMS solu- e.g. new flow controller and time management for tions for every analytical challenge. maintenance A wide variety of optional products supports trace analysis such as autosamplers and inlets www.shimadzu.eu/coupling-powers CONTENTS August | 2019 Volume 32 Number 8 COVER STORY 396 Development of Fully Automated Bubble-in-Drop Microextraction–Gas Chromatography–Mass Spectrometry of Organochlorine Pesticides in Environmental Waters Claire A. Taylor, Hui Min Lee, and Hian Kee Lee This article describes the intentional incorporation of an air bubble into a solvent droplet for fully automated bubble-in-drop microextraction–gas chromatography–mass spectrometric (BID-GC–MS) analysis of selected organochlorine pesticides as model analytes. FEATURES (HPLC) modules (pump, autosampler, UV detector, and chromatography data system) to be published 420 Sizing Up Size-Exclusion Chromatography in 2019. This instalment provides an overview Alasdair Matheson for analytical-scale HPLC pumps, including their André Striegel spoke to LCGC Europe about requirements, modern designs, operating principles, innovations in size-exclusion chromatography (SEC) in trends, and best practices for trouble-free operation. polymer analysis, including the benefits of hyphenating Recommendations for selection and guidelines for SEC with on-line multi-angle static light scattering operating and troubleshooting are provided. (MALS) and differential refractometry (DRI) detection, and the role of quintuple detection. DEPARTMENTS 424 Company Profiles COLUMNS A selection of profiles on leading separation science 405 LC TROUBLESHOOTING companies. Pass the Salt: Evolution of Coupling Ion-Exchange 454 Products Separations and Mass Spectrometry Dwight R. Stoll 456 Events At the recent HPLC meeting in Milan, Dwight Image credit: Denis Gladkiy/stock.adobe.com Stoll noted several topics of interest to the “LC Troubleshooting” readers. Here, he focuses on the first: MS-friendly ion-exchange separations. 410 PERSPECTIVES IN MODERN HPLC Modern HPLC Pumps: Perspectives, Principles, and Practices Konstantin Shoykhet, Ken Broeckhoven, and Michael W. Dong This instalment is the first of a series of four white papers on high performance liquid chromatography 394 LCGC Europe August 2019 EDITORIAL ADVISORY BOARD Group Vice President Project Manager Daniel W. Armstrong Huba Kalász Mike Tessalone Vania Oliveira University of Texas, Arlington, Texas, USA Semmelweis University of Medicine, Günther K. 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Fell Mary Ellen McNally Sales Executive Pharmaceutical Chemistry, University of DuPont Crop Protection, Newark, Liz Mclean Bradford, Bradford, UK Delaware, USA Hinderton Point, Lloyd Drive, [email protected] Attila Felinger Imre Molnár Cheshire Oaks, Professor of Chemistry, Department of Molnar Research Institute, Berlin, Germany Analytical and Environmental Chemistry, Cheshire, CH65 9HQ, UK Luigi Mondello Senior Director, Digital Media University of Pécs, Pécs, Hungary Dipartimento Farmaco-chimico, Facoltà Michael Kushner Tel. +44 (0)151 353 3500 Francesco Gasparrini di Farmacia, Università di Messina, [email protected] Fax +44 (0)151 353 3601 Dipartimento di Studi di Chimica e Messina, Italy Tecnologia delle Sostanze Biologicamente Peter Myers Attive, Università “La Sapienza”, Rome, Department of Chemistry, University of Webcast Operations Manager Italy Liverpool, Liverpool, UK Joseph L. 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