Catalog # Aliquot Size F37-911-05 3 x 5 nmol F37-911-20 3 x 20 nmol F37-911-50 3 x 50 nmol FKBP1A siRNA Set I siRNA duplexes targeted against three exon regions Catalog # F37-911 Lot # Z2037-46 Specificity Formulation FKBP1A siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human FKBP1A expression. shipped at room temperature. Product Description Reconstitution Protocol FKBP1A siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human FKBP1A mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_000801. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot FKBP12, FKBP1, PKC12, PKCI2, PPIASE, FKBP-12, FKBP12C 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or FKBP1A Protein F37-30G below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background FKBP1A is a member of the immunophilin protein family FKBP1A siRNA Set I which plays a role in immunoregulation and basic cellular siRNA duplexes targeted against three exon regions processes involving protein folding and trafficking. FKBP1A is a cis-trans prolyl isomerase that binds the Catalog Number F37-911 immunosuppressants FK506 and Rapamycin. FKBP1A Specific Lot Number Z2037-46 interacts with various type I receptors, including the TGF- Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol beta type I receptor and competitive binding assays Format Lyophilized powder show that the type I receptor may be a natural ligand for Stability 1yr at -70oC from date of shipment FKBP1A (1). FKBP1A-deficient mice have normal skeletal Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is muscle but show severe dilated cardiomyopathy and recommended that the lyophilized ventricular septal defects that mimick a human and resuspended siRNAs are stored at congenital heart disorder (2). or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. Wang, T. et.al: Specific interaction of type I receptors long-term storage, it is recommended that the siRNA is stored at -70oC. For of the TGF-beta family with the immunophilin FKBP-12. most favorable performance, avoid Science 265: 674-676, 1994. repeated handling and multiple 2. Shou, W. et al: Cardiac defects and altered freeze/thaw cycles. ryanodine receptor function in mice lacking FKBP12. Nature 391: 489-492, 1998. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .