PROTOZOOLOGICAACTA ''V.' i -XhM'i- i i ' ; m % & . / M ' J.JsSw. v.. w...... A .. ..sSSL. ..3 . - ,«.. REDACTORU M CONSILIUM S. DRYL (WARSZAWA), A. GRĘBECKI (WARSZAWA), O. JlROVEC (PRAHA), G. I. POLJANSKY (LENINGRAD), Z. RAABE (WARSZAWA), K. M. SUKHANOVA (LENINGRAD) VOLUMEN Vir Fasciculi: 1 — 10 WARSZAWA 19 6 9 http://rcin.org.pl INSTYTUT BIOLOGII DOŚWIADCZALNEJ IM. M. NENCKIEGO POLSKIEJ AKADEMII NAUK ACTA PROTOZOOLOGICA Redaktor Naczelny: Zastępca Redaktora Naczelnego: Sekretarz Redakcji ZDZISŁAW RAABE STANISŁAW DRYL STANISŁAW L. KAZUBSKI NOTICE TO AUTHORS Acta Protozoologica is intended as a journal serving for the publication of original papers embodying the results of experimental or theoretical research in ail fields of protozoology with the exception of purely clinical reports. The papers must be concise and will not be accepted if they have been previously published elswhere. 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Manuscripts may be submitted to each member of the Editorial Board or direc- tly to the Office: Acta Protozoologica, Nencki Institute of Experimental Biology. Warszawa 22, ul. Pasteura 3, Poland. http://rcin.org.pl ACTA PROTOZOOLOGICA VOL. VII WARSZAWA, 30.IX.1969 FASC. 1 Institute of Zoology, Warsaw University, Warszawa 64, Poland J. KACZANOWSKA and D. KOWALSKA Studies on topography of the cortical organelles of Chilodonella cucullulus (O.F.M.) I. The cortical organelles and intraclonal dimorphism Studia nad topografią organelli kortykalnych Chilodonella cucullulus (O.F.M.) I. Organelle kortykalne i dymorfizm wewnątrzklonalny The aim of the present series of studies is a tentative description of cortical organelles disposition on the flat ventral surface of Chilodonella cucullulus (O.F.M.) The studies include both the interdivisional period, as well, as the stages of the development of offspring structures in the parental pattern. The present paper contains the result of the first section of studies. The papers which will follow, would deal with the relation of the total number of kineties to the CVPs (contractile vacuole pores) disposition (Kowalska and Kaczanowska 1969 in press) and the geometry with topography of the cortical organelles. We would like to thank Prof. Dr. Z. Raabe for valuable comments and critisism of the manuscript. • Materials and methods Studies were carried out on stocks marked B, C, S and clone Bj of Chilodonella cucullulus (O.F.M.) isolated from mass cultures from the sewage treatment centre at Józefów near Warsaw. Stock were isolated in March 1967. Stock C died out in May of the same year, while stock S died out in April 1968. Stock B underwent conjugation in the fall of 1967 and from one exconjugant from one caryonide clone Bx was isolated. In this clone mass intraclonal conjugation is periodically observed (experimentally tested by S. Radzikowski M. Sc. unpublished). After the mass conjugation of Bx exconjugants were not re-isolated and the conventional mark Bx is applied for each subclone or substocks isolated from the periodically conjugating pool of initial clone Bx. This pool Bx is cultivated till the present time. Protozoans are cultivated in room temperature in Petri dishes in a small amount of tap water to which a drop of fresh baker yeast suspension is added. The protozoans are fed every second day and inoculated every 4—6-th http://rcin.org.pl ACTA PROTOZOOLOGICA VOL. VII WARSZAWA, 30.IX.1969 FASC. 1 Institute of Zoology, Warsaw University, Warszawa 64, Poland J. KACZANOWSKA and D. KOWALSKA Studies on topography of the cortical organelles of Chilodonella cucullulus (O.F.M.) I. The cortical organelles and intraclonal dimorphism Studia nad topografią organelli kortykalnych Chilodonella cucullulus (O.F.M.) I. Organelle kortykalne i dymorfizm wewnątrzklonalny The aim of the present series of studies is a tentative description of cortical organelles disposition on the flat ventral surface of Chilodonella cucullulus (O.F.M.) The studies include both the interdivisional period, as well, as the stages of the development of offspring structures in the parental pattern. The present paper contains the result of the first section of studies. The papers which will follow, would deal with the relation of the total number of kineties to the CVPs (contractile vacuole pores) disposition (Kowalska and Kaczanowska 1969 in press) and the geometry with topography of the cortical organelles. We would like to thank Prof. Dr. Z. Raabe for valuable comments and critisism of the manuscript. Materials and methods Studies were carried out on stocks marked B, C, S and clone of Chilodonella cucullulus (O.F.M.) isolated from mass cultures from the sewage treatment centre at Józefów near Warsaw. Stock were isolated in March 1967. Stock C died out in May of the same year, while stock S died out in April 1968. Stock B underwent conjugation in the fall of 1967 and from one exconjugant from one caryonide clone B: was isolated. In this clone mass intraclonal conjugation is periodically observed (experimentally tested by S. Radzikowski M. Sc. unpublished). After the mass conjugation of Bx exconjugants were not re-isolated and the conventional mark Bx is applied for each subclone or substocks isolated from the periodically conjugating pool of initial clone B^ This pool Bi is cultivated till the present time. Protozoans are cultivated in room temperature in Petri dishes in a small amount of tap water to which a drop of fresh baker yeast suspension is added. The protozoans are fed every second day and inoculated every 4—6-th http://rcin.org.pl 2 J. KAC'ZANOWSK A and D. KOWALSKA day. The sporadic infection of cultures with Chilodonella uncinata, or fungi was only removed by means of re-isolation of protozoans. The cultures used in experiments were started from a single ciliate isolated from the stock cultures. The infravital observations were conducted by contrast phase microscopy. Observations were also made of stained ciliates with nigrozin and silvered after Klein and most of them after Chatton-Lwoff in Corliss (1953) modifica- tion. Three series of experiments with protozoans of the same age were con- ducted on stock S and clone Bx in a period when the ciliates were in good physiological condition. Individual samples contained about 10 dividers were isolated during five minutes in stage of advanced cytokinesis. The process of cytokinesis was completed in isolated specimens within 5 to 8 min.