AMC 2019 Oral Session 13 [Diversity of entomopathogenic fungi] 3-O13-3 Harpellales from Japan by wider host family collection Hiroki Sato Forestry and Forest Products Research Institute, Japan Harpellales is an order in the Kickxellomycotina which are living in the gut of aquatic insects, especially juveniles of Ephemeroptera, Plecoptera and Diptera. Over 250 species have been described to date. Purpose: We are studying to clarify the species diversity of Harpellales in Japan comparing with those of Asian countries. Methods: Literature research. Observation of the fungi in the guts of aquatic insects by dissection. Results and conclusion: Forty nine species have been recoded from Asia. Thirty one species in 15 genera, are known from China, 20 species in 12 genera from Japan, 7 species in 5 genera from India, and 5 species in 4 genera from Thailand. Only Harpella melusinae: is recorded from all the four countries. To compare with the species number, genus number is not so much lower than China. This could reflect the larger number of host families being examined. The families investigated in China: Simuliidae, Chironomidae, Tipulidae and Culicidae in Diptera; Baetidae, Ephemerellidae and Heptageniidae in Ephemeroptera; Plecoptera sp. In Japan, in addition to the families researched in China, harpellalean species have been recorded from Blephariceridae and Thaumaleidae in Diptera, Leptophlebiidae in Ephemeroptera, and Nemouridae in Plecoptera. In this study, we explored to examine not or less investigated host families in Japan. We found about 20 more harpellalean species from Capniidae, Chloroperlidae, Peltoperlidae and Taeniopterygidae in Plecoptera, Caenidae, Ephemerellidae and Isonychiidae in Ephemeroptera. In total about 40 species are recognized suggesting a wide diversity in Japan. Asian Mycological Congress 2019.