ERLIN2 Antibody Cat. No.: 45-552 ERLIN2 Antibody 45-552 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Specifications HOST SPECIES: Goat SPECIES REACTIVITY: Human HOMOLOGY: Expected Species Reactivity based on sequence homology: Rat IMMUNOGEN: The immunogen for this antibody is: C-EDEPLETATKEN TESTED APPLICATIONS: ELISA, IHC, WB Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 43kDa band observed in Human Breast lysates and in lysates of Human Breast Cancer (calculated MW of 37.8kDa according to NP_009106.1). The observed molecular weight corresponds to APPLICATIONS: earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml. September 26, 2021 1 https://www.prosci-inc.com/erlin2-antibody-45-552.html SPECIFICITY: This antibody is expected to recognise isoform 1 (NP_009106) only. POSITIVE CONTROL: 1) Cat. No. 1311 - Human Breast Tissue Lysate Properties Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing. CONCENTRATION: 500 ug/mL STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing. Additional Info OFFICIAL SYMBOL: ERLIN2 ERLIN2, SPFH2, ER lipid raft associated 2, SPFH domain family, member 2 , HGNC:1356, ALTERNATE NAMES: C8orf2, MGC87072 , chromosome 8 open reading frame 2, Erlin-2, MGC87072 ACCESSION NO.: NP_009106.1 PROTEIN GI NO.: 6005721 GENE ID: 11160 Background and References 1) Garcia MJ, Pole JC, Chin SF, Teschendorff A, Naderi A, Ozdag H, Vias M, Kranjac T, Subkhankulova T, Paish C, Ellis I, Brenton JD, Edwards PA, Caldas C. A 1 Mb minimal REFERENCES: amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes. Oncogene. 2005 Aug 4;24(33):5235-45. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 2 https://www.prosci-inc.com/erlin2-antibody-45-552.html.