NSF Engineering Research Center for Biorenewable Center for Biorenewable Chemicals Annual Reports Chemicals 4-7-2011 NSF Engineering Research Center for Biorenewable Chemicals, Third Year Renewal Proposal, Volume II NSF Engineering Research Center for Biorenewable Chemicals Follow this and additional works at: http://lib.dr.iastate.edu/cbirc_annualreports Part of the Biomedical Engineering and Bioengineering Commons, and the Chemical Engineering Commons Recommended Citation NSF Engineering Research Center for Biorenewable Chemicals, "NSF Engineering Research Center for Biorenewable Chemicals, Third Year Renewal Proposal, Volume II" (2011). Center for Biorenewable Chemicals Annual Reports. 5. http://lib.dr.iastate.edu/cbirc_annualreports/5 This Book is brought to you for free and open access by the NSF Engineering Research Center for Biorenewable Chemicals at Iowa State University Digital Repository. 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NSF Engineering Research Center for Biorenewable Chemicals Transforming the THIRD YEAR RENEWALchemical PROPOSALindustry for a sustainable future VOLUME II April 7, 2011 Dr. Brent Shanks, Director Dr. Basil Nikolau, Deputy Director Core Partner Institutions Iowa State University (Lead) Rice University University of California, Irvine University of New Mexico University of Virginia University of Wisconsin Transforming the chemical industry for a sustainable future A National Science Foundation Engineering Research Center since 2008 Cooperative Agreement No. EEC-0813570 CBiRC Third Year Renewal Proposal Table of Contents Table of Contents ........................................................................................................................... i List of Projects................................................................................................................................1 Thrust 1 – New Biocatalysts for Pathway Engineering .............................................................1 Thrust 2 – Microbial Metabolic Engineering ............................................................................3 Thrust 3 – Chemical Catalyst Design ........................................................................................7 Life Cycle Assessment .............................................................................................................12 Pre-College Education .............................................................................................................12 University Education ...............................................................................................................14 International Education ............................................................................................................15 Project Summaries (for Center-controlled or Core Projects) ......................................................17 Thrust 1 – New Biocatalysts for Pathway Engineering ...........................................................17 Thrust 2 – Microbial Metabolic Engineering ..........................................................................45 Thrust 3 – Chemical Catalyst Design ......................................................................................97 Life Cycle Assessment ...........................................................................................................139 Pre-College Education ...........................................................................................................144 University Education .............................................................................................................159 International Education ..........................................................................................................166 Associated Project Abstracts ....................................................................................................171 Thrust 1 – New Biocatalysts for Pathway Engineering .........................................................171 Thrust 2 – Microbial Metabolic Engineering ........................................................................181 Thrust 3 – Chemical Catalyst Design ....................................................................................190 Life Cycle Assessment Support Area ....................................................................................201 Pre-College Education ...........................................................................................................202 University Education .............................................................................................................203 International Education ..........................................................................................................204 Data Management Plan .............................................................................................................205 Bibliography of Publications .....................................................................................................209 Biographical Sketches ................................................................................................................217 Current and Pending Support ..................................................................................................271 Volume II i April 7, 2011 CBiRC Third Year Renewal Proposal Volume II ii April 7, 2011 CBiRC Third Year Renewal Proposal List of Projects Thrust 1 New Biocatalysts for Pathway Engineering Faculty Investigators Department Institution CENTER-CONTROLLED (CORE) PROJECTS T1.1 – 3-ketoacyl-ACP Synthase: Characterization of Novel Biocatalysts (3-ketoacyl Synthases) for Diversifying FAS/PKS Metabolic Pathways Joseph P. Noel (Lead) Jack H. Skirball Center for Salk Institute for Biological Chemical Biology & Studies Proteomics Basil J. Nikolau Biochemistry, Biophysics & Iowa State University Molecular Biology Eran Pichersky Molecular, Cellular & University of Michigan Developmental Biology Peter J. Reilly Chemical & Biological Iowa State University Engineering T1.2 – Acetoacetyl-CoA: Use of Escherichia coli for the Production of Molecules Functionalized for Chemical Synthesis Thomas A. Bobik (Lead) Biochemistry, Biophysics & Iowa State University Molecular Biology Basil J. Nikolau Biochemistry, Biophysics & Iowa State University Molecular Biology T1.3 – Acetyl-CoA/Propionyl-CoA Synthetase: Biocatalysts for Diversifying Precursor Pools for FAS/PKS Systems David J. Oliver (Lead) Genetics, Development & Cell Iowa State University Biology Peter J. Reilly Chemical & Biological Iowa State University Engineering T1.4 – Acyl-CoA Carboxylases: Biocatalysts for Diversifying Precursor Pools for FAS/PKS Systems Basil J. Nikolau (Lead) Biochemistry, Biophysics & Iowa State University Molecular Biology Peter J. Reilly Chemical & Biological Iowa State University Engineering T1.5 – Methylketone Synthase/Thioesterase: Development of Methylketone Synthase Enzyme Adapted for the Production of Short-Chain Methylketones Eran Pichersky (Lead) Molecular, Cellular & University of Michigan Developmental Biology Joseph P. Noel Jack H. Skirball Center for Salk Institute for Biological Chemical Biology & Studies Proteomics Volume II 1 April 7, 2011 CBiRC Third Year Renewal Proposal T1.6 – Thioesterases: Characterization of Novel Biocatalysts (Thioesterases) for Diversifying FAS/PKS Metabolic Pathways Basil J. Nikolau (Lead) Biochemistry, Biophysics & Iowa State University Molecular Biology Peter J. Reilly Chemical & Biological Iowa State University Engineering ASSOCIATED PROJECTS A Genetically Tractable Microalgal Platform for Advanced Biofuel Production U. S. Department of Energy Basil J. Nikolau Biochemistry, Biophysics & Iowa State University Molecular Biology David J. Oliver Genetics, Development & Cell Iowa State University Biology Eve S. Wurtele Genetics, Development & Cell Iowa State University Biology Advancing Drug Development from Medicinal Plants Using Transcriptomics and Metabolomics National Institutes of Health Eve S. Wurtele (Lead) Genetics, Development & Cell Iowa State University Biology Basil J. Nikolau Biochemistry, Biophysics & Iowa State University Molecular Biology Annotation of Novel Enzymatic Functions in Methanogens U. S. Department of Energy Basil J. Nikolau (Lead) Biochemistry, Biophysics & Iowa State University Molecular Biology Biocatalysts of the Acetyl-CoA Condensation Metabolic Pathway Iowa Board of Regents (Battelle Fund) Basil J. Nikolau (Lead) Biochemistry, Biophysics & Iowa State University Molecular Biology Biosynthesis of Alkamides – Experimental Modeling of a Modular Secondary Metabolic Pathway National Science Foundation Basil J. Nikolau (Lead) Biochemistry, Biophysics & Iowa State University Molecular Biology Coenzyme B12-dependent 1,2-propanediol Degradation in Salmonella National Science Foundation Thomas A. Bobik (Lead) Biochemistry, Biophysics & Iowa State University Molecular Biology Collaborative Research: Structural, Functional and Evolutionary Basis for the Utilization of a Quinone Methide-Like Mechanism in the Biosynthesis of Plant Specialized Metabolites National Science Foundation Joseph P. Noel (Lead) Jack H. Skirball Center for Salk Institute for Biological Chemical Biology & Studies Proteomics Volume II 2 April 7, 2011 CBiRC Third Year Renewal Proposal Eran Pichersky Molecular, Cellular & University