Vitae ISSN: 0121-4004 [email protected] Universidad de Antioquia Colombia FREITAS-NUNES, Cristina; GOMES DE SOUSA-CARDOZO, Francielle Tramontini; NASSER, Paulo NASSER; SCASSI, Felipe; GERHARDT, Deborah; CAMELINI, Carla Maisa; ROSSI, Márcio José; CUNHA-PETRUS, José Carlos; CERDEIRA-SABINO, Ester; MARCUCCI-RIBEIRO, Maria Cristina; MALTA-ROMANO, Camila ANTIVIRAL SCREENING OF EXTRACTS DERIVED FROM PLANT AND BACTERIA Vitae, vol. 21, núm. 1, 2014, pp. S107-S108 Universidad de Antioquia Medellín, Colombia Available in: http://www.redalyc.org/articulo.oa?id=169831208053 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative VITAE, REVISTA DE LA FACULTAD DE QUÍMICA FARMACÉUTICA ISSN 0121-4004 / ISSNe 2145-2660. Volumen 21 suplemento 1, año 2014 S107 Universidad de Antioquia, Medellín, Colombia ANTIVIRAL SCREENING OF EXTRACTS DERIVED FROM PLANT AND BACTERIA Cristina FREITAS-NUNES1; Francielle Tramontini GOMES DE SOUSA-CARDOZO1, Paulo NASSER1; Felipe SCASSI1; Deborah GERHARDT1; Carla Maisa CAMELINI2; Márcio José ROSSI3; José Carlos CUNHA-PETRUS2; Ester CERDEIRA-SABINO1; Maria Cristina MARCUCCI-RIBEIRO4 and Camila MALTA-ROMANO1. BACKGROUND Dengue is a major public health problem, affecting all tropical and many subtropical countries. It has been reported more than 200 thousand cases per year only in Americas (1). Sixty nine percent (69 %) of anti-infective drugs developed between 1981 and 2010 had been derived from or inspired by natural products, highlighting the importance of the natural resources in the research and development of new antiviral drugs (2). OBJECTIVE In this sense, this study aimed to evaluate the cytotoxicity and the in vitro anti-dengue activity of an Ethanolic Extract of Green Propolis (EEGP) (3) and an Exopolysaccharide extracted from liquid culture of Lactobacillus plantarum (ELP) (ATCC 8014). METHODS Cytotoxicity of EEGP and ELP was evaluated in C6/36 cells by MTT assay. Anti-dengue activity (DENV-2 American strain) of two different non-cytotoxic concentrations of EEGP (15 and 7.5 µg/mL) and ELP (250 and 125 µg/mL), added before or after virus inoculation, was evaluated by tittering cell culture supernatants by quantitative PCR at 48 and 72 h post infection. RESULTS AND CONCLUSION As results and conclusion, the 50% cytotoxicity (CC50) obtained values for EEGP and ELP were > 15 µg/mL and > 250 µg/mL, respectively. Although no anti-DENV-2 activity was found for EEGP or ELP, at the tested concentrations in C6/36 cell model, ongoing studies are in course in order to assess their potential anti-dengue activity on mammalian cell lines, as well as their indirect antiviral effects by immunomodulatory mechanisms. Keywords: Dengue; antiviral agents; propolis; Lactobacillus plantarum. 1 Institute of Tropical Medicine, University of São Paulo; 05403000; São Paulo-SP, Brazil. [email protected] 2 Department of Chemistry and Food Engineering, University of Santa Catarina, 88040-900, Florianópolis-SC, Brazil. 3 Department of Microbiology, Immunology and Parasitology, University of Santa Catarina, 88040-900, Florianópolis-SC, Brazil. 4 Department of Pharmacy, Anhanguera Bandeirante University, 02071013, São Paulo-SP, Brazil. S108 Volumen 21 (Supl. 1); 2014 REFERENCES 1. Programa Regional da dengue da OPAS/OMS, PWR/COR, Dados da dengue nas Américas, 2013. Available from: http://www.paho.org/bra/ index.php?option=com_content&view=article&id=3168&Itemid=99999. Data accessed: 29/02/2014. 2. Cragg GM, Newman DJ. Natural products: A continuing source of novel drug leads. Biochim Biophys Acta. 2013 Jun;1830(6):3670-95 3. Nunes CF, Finger PF, Fischer G, De Castro CC, Hubner SO, Paulino N , et al. Padronização de uma amostra de extrato etanólico de própolis verde. Revista Fitos. 2012; 7(1): 63-73..