Dement Neuropsychol 2015 December;9(4):428-432 Case Report Cadasil – genetic and ultrastructural diagnosis Case report Julio Cesar Vasconcelos da Silva1, Leila Chimelli2, Felipe Kenji Sudo3, Eliasz Engelhardt4 ABSTRACT. Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is a hereditary disorder which affects the cerebral vasculature due to mutations in the NOTCH 3 gene. The diagnosis may be established through genetic testing for detection of these mutations and/or by skin biopsy. We report a case of the disorder in a female patient, who presented recurrent transient ischemic attacks that evolved to progressive subcortical dementia. Neuroimaging disclosed extensive leukoaraiosis and lacunar infarcts. The genetic analysis for NOTCH 3 was confirmatory. The ultrastructural examination of the skin biopsy sample, initially negative, confirmed the presence of characteristic changes (presence of granular osmiophilic material inclusions [GOM]), after the analysis of new sections of the same specimen. The present findings indicate that negative findings on ultrastructural examinations of biopsy should not exclude the diagnosis of the disease and that further analyses of the sample may be necessary to detect the presence of GOM. Key words: CADASIL, skin biopsy, granular osmiophilic material, NOTCH 3. CADASIL: DIAGNÓSTICO GENÉTICO E ULTRAESTRUTURAL. RELATO DE CASO RESUMO. Arteriopatia Cerebral Autossômica Dominante com Infartos Subcorticais e Leucoencefalopatia (CADASIL) é uma desordem hereditária que acomete a vasculatura cerebral devido a mutações no gene do NOTCH 3. O diagnóstico pode ser estabelecido através de testes genéticos para a detecção das mutações e/ou de biópsia de pele. É relatado um caso da desordem em paciente feminino, que apresentou ataques isquêmicos transitórios recorrentes, evoluindo para um quadro progressivo de demência subcortical. A neuroimagem mostrou extensa leucoaraiose e infartos lacunares. A análise genética para NOTCH 3 foi confirmatória. O exame ultraestrutural de biópsia de pele, inicialmente negativa, confirmou a presença de alterações características (presença de material granular osmiofílico [GOM]), após a análise de novos cortes do mesmo material. Os presentes achados indicam que achados negativos de exames ultraestruturais de biópsia não deveriam excluir o diagnóstico da doença e que análise adicional da amostra pode ser necessária para detectar a presença de GOM. Palavras-chave: CADASIL, biópsia de pele, material granular osmiofílico, NOTCH 3. INTRODUCTION episodes, psychiatric and neurological symp- erebral Autosomal Dominant Arteriopa- toms and cognitive impairment, and ulti- Cthy with Subcortical Infarcts and Leuko- mately dementia.1,2 encephalopathy (CADASIL) is an early-onset Several methods for diagnosing CADASIL vascular disorder associated with recurrent have been proposed, including genetic testing, subcortical transient ischemic attacks (TIA), Magnetic Resonance Imaging (MRI) and usually preceded or accompanied by migraine skin biopsy. The first descriptions of MRI This study was conducted at the Universidade Federal do Rio de Janeiro (UFRJ). 1Neuropsychologist; MSc in Internal Medicine/Neurology- x; PhD Student at the Institute of Psychiatry, UFRJ, Rio de Janeiro - Brazil. 2Professor at the Department of Pathology - UFRJ, Rio de Janeiro - Brazil. 3MD, MSc In Psychiatry - Institute of Psychiatry; PhD Student at the Institute of Psychiatry - UFRJ, Rio de Janeiro - Brazil. 4Full Professor (retired) - Federal University of Rio de Janeiro; Cognitive and Behavioral Neurology Unit - Institute of Neurology Deolindo Couto and Center for Alzheimer’s Disease/Institute of Psychiatry - UFRJ, Rio de Janeiro - Brazil. Julio Cesar Vasconcelos da Silva. Rua Garibaldi, 225 / 107 / Bl 2 – 20511-330 Rio de Janeiro RJ – Brazil. E-mail: [email protected] Disclosure: The authors report no conflits of interest Received September 09, 2015. Accepted in final form November 12, 2015. 428 Neuroimaging in CADASIL Silva et al. Dement Neuropsychol 2015 December;9(4):428-432 abnormalities in CADASIL date from 1991.3,4 Subcortical Table 1. Clinical aspects throughout the patient’s life. ischemic lesions (leukoencephalopathy), hypointense Clinical features Age (years) on T1 and hyperintense on T2-weighted and FLAIR Migraine 19 images, are characteristic findings. In early stages, these Paresthesia in lower left limb 42 lesions are predominantly located in the periventricular Paresthesia spread to the upper left limb, and to the 44 regions, centrum semiovale, temporal white matter and right limbs; left-side hemiparesis; dysphagia; vertigo; external capsule, while lacunar infarcts, are also common cognitive impairment (aphasia [transient]; dysnomia; early in the disease.5,6 Although many patients develop forgetfulness for recent events, “distorted vision”) symptoms before 60 years of age, MRI changes may Mood disorders (depressive symptoms, anxiety) 45 4 occur before 35 years of age. Right-sided hemiparesis 46 Another noteworthy diagnostic method for Pseudobulbar palsy; aphasia 53 CADASIL is ultrastructural investigation for granular Dementia and death 57 osmiophilic material (GOM) deposits in the smooth muscle cells of arterioles, obtained through biopsy (skin or muscle). Besides genetic testing for NOTCH 3 and the identification of the NOTCH 3 receptor by (anomia), memory (forgetfulness for recent events), immunohistochemistry, ultrastructural analysis of GOM visuospatial and visuoperceptual abilities (complaints can detect the presence of the disease in cases where the of “distorted vision”) were perceived during succes- cited methods are not available. sive consultations with the psychologist. The cogni- The present article reports a case of CADASIL in tive impairments became more severe and progressed which the diagnosis, previously confirmed by genetic to dementia, and the patient died at the age of 57. The testing, was ratified after repeated analyses of a skin Table below depicts the main clinical features presented biopsy sample, with the aim of: (i) discussing the by the patient, according to medical records. diagnostic methods; and (ii) highlighting the importance Brain MRI at age 56 showed extensive white matter of reiterated analyses of a skin biopsy, even when initially lesions and numerous lacunar infarcts, and dilated negative, in search of the ultrastructural marker of supratentorial ventricles (Figure 1). CADASIL. Initially, a number of different disorders were considered. However, familial history of TIA, migraine CASE REPORT and white matter lesions indicated that investigation for A female patient presented with complaints of migraine CADASIL should be carried out. When the patient was episodes preceded by visual aura, since the age of 19. At 49, skin biopsy was performed. The sample was analyzed 42, after one such episode, she reported paresthesia and by transmission electron microscopy, which revealed presented hemiplegia. Despite the lack of formal neuro- slight thickening of the wall of arterioles; however no psychological assessment, impairments in language GOM bodies were visualized in arteriolar walls. Despite Figure 1. Brain MRI performed at 56 years of age. Upper images [1 to 4] - T1-weighted: [A] brainstem lesion, [B and D] lacunes, [C] white- matter hypointensities, [E] small infarct. Lower images [5 to 6] - FLAIR: [F] hyperintensities in the anterior temporal region, [G] hyperintensity in the external capsule, [H] periventricular hyperintensities, [I] hyperintensities in the centrum semiovale. Silva et al. Neuroimaging in CADASIL 429 Dement Neuropsychol 2015 December;9(4):428-432 the negative result, the hypothesis of CADASIL was not commonly followed by cortical-subcortical lesions and rejected, and, in 2006, DNA and/or blood samples were disabling manifestations, such as gait disorder, pseudo- sent to France, for genetic analyses,7 as detailed in the bulbar palsy and cognitive impairment, among others.8,9 Box. The mean age for occurrence of these clinical features is around 42 years old,2 ranging from 20 to 65 years.10,11 Box. Description of the genetic analysis performed in the present case. The present study describes the presence of these symp- Samples of DNA and/or blood from the patient and her siblings were toms, similarly to a previous study.7 sent to the Genetics Laboratory of the Lariboisière Hospital, Paris(*). The clinical course was marked by migraine episodes, The sample of the present case comprised 30 μg of a concentrate considered one of the most characteristic symptoms of containing about 200 ng/μl of DNA. The analysis was performed by the disease. A previous study revealed that 22% had direct sequencing of the DNA of exons 3 and 4 of NOTCH 3 (Chromo- migraine with aura.2 The first complaints may appear some 19), which revealed a nucleotide substitution of one arginine (CGC) for one cysteine (TGC) at exon 4 in position 153 (c.535 C>T: before the age of 20. Hence, these symptoms may occur R153C). earlier than the mean age in which brain lesions are evident on MRI images.12 In line with a previous study, The examiners concluded that the mutation was typical of CADASIL. these symptoms were also evident in the present case.7 *Department: Genetics of vascular disorders. Principal Investigator: Additionally, as suggested by Markus et al.,5 white Elisabeth Tournier-Lasserve. Team: Dominique Chiarasini; Anne Jou- matter changes in the anterior temporal pole and in tel; Florence Marchelli; Florence Riant; Manuèle Ruas