US 2008O132691A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2008/0132691 A1 KhVOrOVa et al. (43) Pub. Date: Jun. 5, 2008 (54) SIRNA TARGETING KINASE INSERT Related U.S. Application Data DOMAIN RECEPTOR (KDR) (63) Continuation-in-part of application No. 10/940,892, filed on Sep. 14, 2004, which is a continuation of (75) Inventors: Anastasia Khvorova, Boulder, CO application No. PCT/US04/14885, filed on May 12, (US); Angela Reynolds, Conifer, 2004, Continuation-in-part of application No. 10/714, CO (US); Devin Leake, Denver, 333, filed on Nov. 14, 2003. CO (US); William Marshall, (60) Provisional application No. 60/426,137, filed on Nov. Boulder, CO (US); Steven Read, 14, 2002, provisional application No. 60/502,050, Denver, CO (US); Stephen filed on Sep. 10, 2003. Scaringe, Lafayette, CO (US) Publication Classification Correspondence Address: (51) Int. C. KALOW & SPRINGUT LLP C7H 2L/02 (2006.01) 488 MADISONAVENUE, 19TH FLOOR (52) U.S. Cl. ....................................................... 536/24.5 NEW YORK, NY 10022 (57) ABSTRACT Efficient sequence specific gene silencing is possible through (73) Assignee: DHARMACON, INC., Lafayette, the use of siRNA technology. By selecting particular siRNAs CO (US) by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for (21) Appl. No.: 11/978,107 silencing genes. Methods, compositions, and kits generated through rational design of siRNAS are disclosed including (22) Filed: Oct. 26, 2007 those directed to nucleotide sequences for KDR. s S & 3 & SSSSSSSSSSS k kD CHC & 17O CLCa H EEA 1-> k O-Actinin-> --, -er is a set sat -100 DynII-> ... : --98 k k PPl set is is see essee. Eps 15 - is 40 YFP-Rab5b- site m50 CALM k 72 PPl - it is one O-Actinin-> is as-is is is a -100 sessessess k 3. Eps15R - 8&is& 8, 8. $ - 30 YFP-Rab5c -> theto e50ess - - 90 PP m) sex is is is a see. 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