<p>Adittional file 1: Table S1. Marker alleles, pedigrees and phenotypes for SCN race 3 in 153 soybean lines entered into the USDA Uniform Soybean Tests for Southern States</p><p>Source of Resistance based on SNP Marker allele‡ extended pedigree G Line name Institution Pedigree Reaction SCN Race 3† S GSM38 M GSM191 1 3 8 3 University of G98-1420 x G05-1102 RR 1.0/1.0 G G G 2011/2012 Peking Georgia H7242 RR University of G98-1420 x G06-2460 RR 1.0/1.0 G G G 2011/2012 Peking Georgia H7242 RR University of R07-6669 Lonoke x R00-33 1.0/3.6 G C C 2012/2013 Peking + PI 88788 Arkansas University of R04-522 Lonoke x P9594 1.0 G C C 2011 Peking + PI 88788 Arkansas University of G96-2272 x G04-2215RR 1.0/5.0 G G G 2011/2012 Peking Georgia H7242 RR University of G99-2678 x G08-3795RR 1.0 G C G 2012 PI 88788 Georgia P97M50 University of G00-3083 x G07-2879 RR 1.0/1.0 G G G 2011/2012 Peking Georgia AGS758RR University of N97-9658 x G02- G08-5122 RR 1.0/1.6 G G G 2012/2013 Peking Georgia G176376 University of G99-4158 x 2011/2012/20 G06-3182 RR 1.0/1.0/1.0 G G G Peking Georgia P97M50 13 University of G95-346 x H7242 G03-1187 RR 1.0 G G G 2011 Peking Georgia RR University of TN09-029 Fowler × Anand 1.0 G G G 2012 Peking Tennessee Clemson SC01-786RR x 2011/2012/20 SC07-786RR 1.0/1.0/1.6 G G G Peking University G00-3213 13 SC07-1518RR Clemson SC01-809RR x 1.0/1.0/3.1 G G G 2011/2012/20 Peking University G99-3211 13 Clemson SC01-809RR x 2011/2012/20 SC07-1490RR 1.0/1.0/1.2 G G G Peking University G99-3211 13 Clemson N97-9658 x 2011/2012/20 SC07-108RR 1.0/3.0/4.7 G C G Peking + PI 88788 University SC01-783RR 13 Clemson G00-3213 x SC07-1029RR 1.0/1.0 G G G 2011/2012 Peking University SC00-643RR Clemson SC01-809RR x SC06-708RR 1.0/1.0 G G G 2011/2012 Peking University G99-3211 Clemson SC01-809RR x SC06-676RR 1.0/1.0 G G G 2011/2012 Peking University G99-3211 Clemson N97-9658 x SC06-301RR 1.0/5.0 G C C 2011/2012 Peking + PI 88788 University SC01-783RR Clemson SC00-603RR x SC05-642RR 1.0 G G G 2011 Peking University SC94-1573 SC92-2482 x {SC92-2482 x Clemson SC01-803 RR [HAGOOD x 1.0/1.0 G G G 2011/2012 Peking University (HAGOOD x BC1RES NIKRR)]} University of R00-1076 x S99- R09-1970 1.0 G G G 2013 Peking Arkansas 2281 University of R07-6654 Lonoke x R00-33 1.0 G C C 2012 Peking + PI 88788 Arkansas University of S99-2281 x UA R07-10244 1.0 G G G 2011 Peking Arkansas 4805 University of S99-2281 x UA R07-10231 1.0 G G G 2011 Peking Arkansas 4805 USDA-ARS, Fowler x JTN-5503 1.0 G G G 2011 Peking Jackson, TN Manokin USDA-ARS, S96-2641 x S97- JTN-5208 1.0 G G G 2011 Peking Jackson, TN 1688-8-LOAM02 USDA-ARS, S95-1908 x JTN-5108 1.0/1.0 G G G 2011/2012 Unknown Jackson, TN Bolivar USDA-ARS, LS94-3207 x S95- JTN-4607 1.0/1.0 G G G 2011/2012 Peking Jackson, TN 1908 JTN-4408 USDA-ARS, S97-1753 x V94- 1.0 G G G 2011 Peking Jackson, TN 0198 USDA-ARS, S97-1688 x V94- JTN-4307 1.0/1.0 G G G 2012/2013 Peking Jackson, TN 0198 R95-2210 x V96- V05-2664 Virginia Tech 1.0/3.0 G C C 2011/2012 Peking + PI 88788 0332 University of S04-8882 x S05- S10-10368 1.0 G G G 2013 Peking Missouri 11400 G00-3213 University of G07-1185 RR x(Boggs RR(2) x 1.0/1.0 G G G 2011/2012 Peking Georgia N97-9658) University of G00-3209 x G03- G08-3282 RR 1.0 G G G 2012 Peking Georgia 952 RR University of G00-3083 x G08-2869 RR 1.0 G G G 2012 Peking Georgia AGS758RR University of G00-3213 x G07-3557 RR 1.0/1.0 G G G 2011/2012 Peking Georgia P97M50 USDA-ARS, 2011/2012/20 JTN-5110 J98-32 x Anand 1.0/1.0/1.0 G G G Peking Jackson, TN 13 University of N97-9658 x G08-4200 RR 1.0/1.0 G G G 2012/2013 Peking Georgia BOGGS RR University of G00-3213 x G10PR-224R2 1.0 G G G 2013 Peking Georgia NC7308A1-COYN USDA-ARS, 2011/2012/20 N7003CN Cook × Anand 1.0/4.0/1.5 G G C Peking Raleigh, NC 13 University of R98-3267F x R06-4475 2.0/5.0 G C C 2012/2013 Peking + PI 88788 Arkansas R97-1832 University of Prichard-RR x G05-4237RR 2.0/2.0 G C G 2011/2012 PI 88788 Georgia G94-3117 University of TN02-303 × S98- TN08-114 3/2.7 G G C 2012/2013 Peking Tennessee 1375 University of TN02-303 × S98- TN08-109 2.0/2.0 G G C 2011/2012 Peking Tennessee 1375 Clemson N97-9658 x SC07-150RR 2.0 G G C 2011 Peking University SC00-643RR Clemson SC98-1930 x SC06-291RR 2.0/4.3 G C G 2012/2013 PI 88788 University SC00-892RR SC06-007RR Clemson SC98-1850 x 2.0/4.3 G G G 2012/2013 Peking University SC00-892RR University of R00-1076 x BA R09-400 2.0 G C C 2012 PI 88788 Arkansas 743303 University of 5601T x R00- R08-47 2.0 G C G 2012 Peking + PI 88788 Arkansas 1940 University of LS96-1631 x R96- R07-5351 2.0 G G G 2012 Unknown Arkansas 3427 University of R01-2373 x R01- R07-1857 2.0 G C G 2012 PI 88788 Arkansas 315 University of S99-2281 x S00- S05-11482 2.0 G G C 2011 Peking Missouri 9985-03 University of S04-8952 x S03- S08-9727 2.0 G C C 2012 Unknown Missouri 1904 University of S04-11681 x S09-14199 2.0 G G G 2012 Unknown Missouri S04-12412 USDA-ARS, 2011/2012/20 JTN-5203 R93-171 x Anand 2.0/2.0/1.0 G G C Peking Jackson, TN 13 University of G00-3209 x G03- G08-3279 RR 2.0/4.0 G G G 2012/2013 Peking Georgia 952 RR Clemson G00-3213 x SC09-142RR 2.1 G G G 2013 Peking University SC00-643RR Clemson G00-3213 x SC09-142RR 2.1 G G G 2013 Peking University SC00-643RR University of TN02-226 x MON TN09- 47,169 2.6 G G C 2013 Peking Tennessee RR2Y Clemson G00-3213 x SC09-102RR 2.7 G G G 2013 Peking University SC00-643RR Clemson G00-3213 x SC09-102RR 2.7 G G G 2013 Peking University SC00-643RR Clemson SC01-809RR x SC09-210RR 2.7 G G G 2013 Peking University G99-3211 Unknown, AgSouth 2011/2012/20 AGS606RR Commercial ../3.0/3.9 G C C PI 88788 Genetics 13 check University of TN09-004 Fowler × Anand 3.0/4.0 G G C 2011/2012 Peking Tennessee TN08-113 University of TN02-303 × S98- 3.0 G G C 2012 Peking Tennessee 1375 Clemson N97-9658 x SC09-092RR 3.0 G G G 2013 Peking University SC00-643RR University of R00-1076 x R00- R08-141 3.0 G C G 2012 PI 88788 Arkansas 1940 University of R00-1551 x R01- R07-1826 3.0/2.6 G G G 2012/2013 Unknown Arkansas 315 University of R05-3239 Ozark x Anand 3.0 G G C 2011 Peking Arkansas University of R00-1194F x S04- S08-14117 3.0 G C C 2012 PI 88788 Missouri 5969RR USDA-ARS, 5601T x N96- N05-7462 3.0 G H H 2011 Unknown Raleigh, NC 6809 Clemson N97-9658 x SC09-092RR 3.0 G G G 2013 Peking University SC00-643RR University of R00-1194F x S03- S08-9942RR 3.3 G C C 2013 PI 88788 Missouri 383RR University of R09-5088 5002T x UA 4805 3.4 G G G 2013 Peking Arkansas University of TN02-226 x MON TN09-44,420 3.4 G G C 2013 Peking Tennessee RR2Y S00-9925-10 x V08-1924 Virginia Tech 3.6 G G C 2013 Peking S00-9985-03 University of S06-7084 x RR2- S10-2635RR 3.8 G G C 2013 Unknown Missouri 6851 University of TN09-008 Fowler × Anand 4.0/1.1 G G C 2012/2013 Peking Tennessee University of TN05-5018 5601T × 5002T 4.0 G G C 2011 Peking Tennessee University of BA 743303 x R09-430 3.0/3.1 G C C 2012/2013 PI 88788 Arkansas R00-684 University of R00-1551 x R01- R07-1810 4.0 G G C 2011 Unknown Arkansas 2346 R95-2210 x V94- V05-2592 Virginia Tech 4.0 G G C 2011 Peking 0436 University of G00-3880 x G10PR-86R2 4.2 G C C 2013 PI 88788 Georgia NC7308A1-COYN University of G00-3880 x G09-3202R2 4.3 G C H 2013 PI 88788 Georgia NC7308A1-COYN Clemson N97-9658 x SC09-052RR 4.3 G G C 2013 Peking University SC00-643RR Clemson N97-9658 x SC09-039RR 4.3 G G H 2013 Peking University SC00-643RR Clemson N97-9658 x SC09-052RR 4.3 G G C 2013 Peking University SC00-643RR University of G93-2225 x (G00- G10PR-56444R2 4.6 G G C 2013 Peking Georgia 3213 x RR2Y) University of G93-2225 x (G00- G10PR-56330R2 4.8 G G C 2013 Peking Georgia 3213 x RR2Y) 99VPI-67 x S00- V08-0136 Virginia Tech 4.9 G G C 2013 Peking 9925-10 Clemson SC98-1850 x SC06-051RR 5.0 G G C 2012 Peking University SC00-892RR R95-2210 x V94- V05-2607 Virginia Tech 5.0 G G C 2011 Peking + PI 88788 0436 University of PRICHARD-RR x AGS828RR 5.0 G C G 2013 PI 88788 Georgia SC96-1476 University of G00-3213(2) x G10PR-56264R2 5.0 G G C 2013 Peking Georgia RR2Y University of S99-2281 x S02- S07-2680 1.0/4.0 T C G 2011/2012 Unknown Missouri 6143 North Late maturing TCHM06-M-204 Carolina State mutant in 2.0/5.0 T C H 2011/2012 Unknown University Holladay M3 University of TN02-226 x MON TN11-4513 3.0 T C H 2013 Peking Tennessee RR2Y University of 5002T x R01- R09-1589 3.0 T C C 2013 Unknown Arkansas 4752 USDA-ARS, N7002 N7001 × Cook 3.0/5.0 T C C 2011/2012 Unknown Raleigh, NC University of PIO 9592 x R03-1250 3.0 H C C 2011 Unknown Arkansas KS4895 S96-2641 x V97- V06-0855 Virginia Tech 3.0 T C C 2011 Unknown 1549 University of DP4748 x S01- R09-4571 3.3 T C C 2013 Unknown Arkansas 9794 University of S04-10364 x S09-9943 3.6 T C C 2013 Unknown Missouri LG04-5196 University of R08-527 UA 4805 x 5002T 3.8 T C C 2013 Unknown Arkansas North N97-61 x TN96- NCC04-619 Carolina State 4.0 T C C 2011 Unknown 64 University University of S98-1375 x R97- R08-1178 4.0 H H C 2012 Unknown Arkansas 1634 University of R06-4433 Lonoke x P9594 4.0 T C C 2011 Unknown Arkansas University of R05-3817 R96-3427 x 605 4.0 T C C 2011 Unknown Arkansas University of R05-374 Lonoke x DP4748 4.0/4.0 T C H 2011/2012 Unknown Arkansas 5002T x V99- V05-2037 Virginia Tech 4.0 T C C 2011 Unknown 3337 V95-0391 x N96- V05-2326 Virginia Tech 4.0 T C C 2011 Unknown 7211 V96-0340 x V94- V06-0283 Virginia Tech 4.0 T C C 2011 Unknown 1401 University of R00-1194F x S04- S08-14087 4.0 T C C 2011 Unknown Missouri 5969RR University of LG04-5196 x S08-17361 4.0 T C C 2011 Unknown Missouri S00-9925-10 BS University of Hartz 5545 x 2011/2012/20 OSAGE 4.0/4.0/.. T C G Unknown Arkansas KS4895 13 North Centennial x 2011/2012/20 Dillon Carolina State 1.0/4.0/4.7 T C G Unknown Young 13 University North N99-8137 x 2011/2012/20 NCC06-1090 Carolina State 3.0/4.0/4.1 T C C Unknown TN99-117 13 University USDA-ARS, 2011/2012/20 NC-ROY Holladay x Brim 4.0/4.0/4.3 T C C Unknown Raleigh, NC 13 USDA-ARS, N7002 x N98- 2011/2012/20 N05-7432 3.0/3.0/4.4 T C C Unknown Raleigh, NC 7265 13 USDA-ARS, N10-7121 ROY x 398833-BB 4.2 T C C 2013 Unknown Raleigh, NC USDA-ARS, N10-7143 ROY x 398976-BB 4.2 T C C 2013 Unknown Raleigh, NC University of 5601T x TN08-100 4.2 T C C 2013 Unknown Tennessee PI417088 USDA-ARS, N98-7961 x N02- N09-13671 4.0/4.9 T C C 2012/2013 Unknown Raleigh, NC 8718 USDA-ARS, 2011/2012/20 N8001 N7001 × Cook 3.0/4.0/3.8 T C C Unknown Raleigh, NC 13 University of S04-20912RR x S09-6201 4.4 T C H 2013 Unknown Missouri S05-4630RR University of 5601T x R01- R10-5721 4.5 T C C 2013 Unknown Arkansas 2195 USDA-ARS, NC-ROY x N02- N09-13690 4.5 T C C 2013 Unknown Raleigh, NC 8718 University of LG04-6863 x S04- S09-13635 4.6 T C C 2013 Unknown Missouri 10364 University of HOLLADAY x TN11-5062 4.7 H H C 2013 Unknown Tennessee MANOKIN University of R10-197 5002T x R04-357 4.7 T C G 2013 Unknown Arkansas University of S00-9925-10 x R10-1288 4.7 T C C 2013 Unknown Arkansas UA 4805 University of UA 4805 x R01- R09-1822 4.7 T C C 2013 Unknown Arkansas 4747RR USDA DT98-9102 x PI DS25-1 4.8 T C C 2013 Unknown Stoneville, MS 587982A University of R97-1634 x V00- 2011/2012/20 R07-10322 2.0/5.0/4.3 H H C Unknown Arkansas 3824 13 USDA-ARS, N07-14182 N7002 x Clifford 5.0 T C C 2012 Unknown Raleigh, NC USDA-ARS, N99-186 x TN99- N08-145 5.0/4.3 H H C 2012/2013 Unknown Raleigh, NC 117 N08-391 USDA-ARS, S99-1171 x N00- 5.0/3.6 T C C 2012/2013 Unknown Raleigh, NC 370 USDA-ARS, G96-2272 x N00- N08-521 5.0/4.4 T C G 2012/2013 Unknown Raleigh, NC 377 University of TN07-754 5601T × 5002T 5.0 T C C 2012 Unknown Tennessee University of R09-209 5002T x Ozark 5.0 T C C 2012 Unknown Arkansas University of R07-1685 5002T x Ozark 5.0 T C C 2012 Unknown Arkansas University of R04-572 MD 4900 x Ozark 5.0 T C C 2011 Unknown Arkansas V97-2276 x V07-5775 Virginia Tech 5.0 T C C 2012 Unknown GP26062 University of S04-10364 x S04- S09-10871 5.0/2.7 T C C 2012/2013 Unknown Missouri 12412 USDA-ARS, N98-7961 x N02- N09-13663 5.0/4.3 T C C 2012/2013 Unknown Raleigh, NC 8718 USDA-ARS, N7002 x N98- N05-7380 5.0 T C C 2012 Unknown Raleigh, NC 7265 North NCC09-200719-1-37 Carolina State Unknown 5.0 T C C 2013 Unknown University North Md 99-6226 x 2011/2012/20 NCC07-8138 Carolina State 4.0/5.0/4.1 T C C Unknown N97-9677 13 University North NCC09-135 Carolina State Unknown 5.0 H H C 2013 Unknown University USDA-ARS, N7002 x N09-13128 5.0/3.6 T C C 2012/2013 Unknown Raleigh, NC TAMAHAKARI-BB USDA-ARS, N09-13189 ROY x PI 5083294 5.0 T C C 2013 Unknown Raleigh, NC North R97-1634 x N97- 2011/2012/20 NCC06-899 Carolina State 2.0/5.0/4.6 T C C Unknown 9693 13 University †The ratings for reactions to race 3 were as follows: 1 = 0-5 cysts, 2 = 6-10 cysts, 3 = 11-20 cysts, 4 = 21-40 cysts, and 5 = > 40 cysts on the root. ‡ “H” represents heterozygote. </p>