<p>Mass spectrometry:</p><p>Responsible person: Ms. S. Fürstenberg and Dr. I. Starke Labor: E 0.11-12 phone: 5294, 5150 Opening hours: 9.00 -9.30 Download requisition sheet: Word.doc</p><p>When submitting a sample for analysis make sure that your requisition sheet is filled out completely and carefully. </p><p>In general, if your sample decomposes while obtaining a melting point, then you will not be able to request a electron impact ionization spectrum (EI). Electrospray ionization (ESI) will be required. </p><p>Usually till 1.5 miligram of your sample are sufficient for an analysis.</p><p>Ionization Techniques available</p><p>Electron impact ionization (EI) is the classical ionization technique in mass spectrometry; however, its application is restricted to thermally stable samples with low molecular mass (m/z 650). An EI spectrum contains many signals of fragment-ions from the compound and is therefore useful for structural characterization and identification of a compound. </p><p>Electrospray ionization (ESI) is the method of choice for proteins, oligosaccharides, oligonucleotides and complexes. However, the sample must be soluble and stable in protic solvents. </p><p>For an overview of the instruments available, see our :</p><p>Instruments</p><p>1. The GC-MS TRACE DSQII is a single quadrupole mass spectrometer equipped with an Electron impact ionization source. Mass range: up to 1050 Da. Direct inlet port and GC ( Low resolution spectra) Concentration of the samples : 1 mg / 100 microliter</p><p>2. The GC-TOF is a Time of flight mass spectrometer equipped with an Electron impact (EI) source. Mass range : up to 1.500 Da, Direct inlet port and GC. Concentration of the samples : about 1 mg</p><p>3. The Q-TOF is a Time of flight mass spectrometer equipped with an Electrospray ionization (ESI) source. Mass range : up to 20 kDa Concentration of the samples : 0.1 - 1.5 mg (exact sample amount is needed)</p>