<p> Hellenic Accreditation System</p><p>Annex F1/8 to the Certificate 493-2</p><p>SCOPE of ACCREDITATION of the National Reference Laboratory for Marine Biotoxins of the Department of Aquatic Organisms Pathology, Control of Marine Biotoxins and Toxins in Other Waters of the Directorate of Veterinary Centre of Thessaloniki of the Ministry of Rural Development and Food </p><p>Materials/Products tested Types of test/Properties measured Applied methods/Techniques used</p><p>Toxicological Testing</p><p>1. Shellfish (bivalve Analysis of lipophilic toxins in shellfish by Test method (ΔΜΤ.01) for molluscs) mouse bioassay. the analysis of lipophilic toxicity in shellfish by mouse Qualitative determination of okadaic acid, bioassay, based on the dinophysistoxins, pectenotoxins, yessotoxins standard method of and azaspiracids. Yasumoto et al. (1978) as recommended by the Detectable lipophlilic toxins concentration of European Union Reference at least 160 µg okadaic acid equivalents/kg Laboratory of Marine and upwards Biotoxins (AESAN, Campus Universitário de Vigo, As Lagoas-Marcosende, Vigo, Spain )</p><p>2. Shellfish (bivalve Analysis of PSP toxicity in shellfish by Test method (ΔΜΤ.02) for molluscs) mouse bioassay the analysis of PSP toxicity in shellfish by mouse Range: at least 400 µg STX diHCl bioassay, based on the equivalents/kg and upwards AOAC official method 959.08.</p><p>Page 1 of 2 Annex F1/8 to the Certificate ESYD No 493-2 09.03.2017 Materials/Products tested Types of test/Properties measured Applied methods/Techniques used</p><p>Chemical Testing</p><p>1. Shellfish and finfish Determination of domoic acid concentration Internal method (ΔΜΤ.03) (ASP toxins). validated according to the requirements of the Decision Range:  1.0 mg of domoic acid 2002/657/EC by the equivalents/kg and upwards. European Union Reference Laboratory of Marine Biotoxins (AESAN, Campus Universitário de Vigo, As Lagoas-Marcosende, Vigo, Spain) by use of High Performance Liquid Chromatography with Ultraviolet Detection by Diode Array Detector (HPLC-UV-DAD)</p><p>Site of assessement: Laboratory permanent premises, 3A Limnou street, 546 27 Thessaloniki, Greece. Approved signatory: T. Zacharaki, Α.Papazachariou.</p><p>This scope of Accreditation replaces the previous one dated 20.10.2016. The Accreditation Certificate 493-2, to ELOT EN ISO/IEC 17025:2005, has been extended until 21.06.2017.</p><p>Athens, March 09, 2016</p><p>Ioannis Sitaras Director of the Laboratories Accreditation Division</p><p>Page 2 of 2 Annex F1/8 to the Certificate ESYD No 493-2 09.03.2017</p>