<p>Name: Date:</p><p>Read Laboratory #1 Osmosis and Diffusion to answer the following Pre-lab Questions.</p><p>The two things that we will be looking at are the processes of osmosis and diffusion and the effect of solute concentration on water potential.</p><p>1. Define the following terms: diffusion</p><p> osmosis</p><p> water potential</p><p>2. What happens at equilibrium?</p><p>3. Define dialysis. Explain what determines if a substance is able to pass through a membrane.</p><p>4. Do you expect glucose to move out of the bag (dialysis tube)? ______Why or why not?</p><p>How will you test this? </p><p>5. Do you expect starch to move out of the bag (dialysis tube)? ______Why or why not?</p><p>How will you test this?</p><p>6. Do you expect water to move out of the bag (dialysis tube)? ______Why or why not? How will you test this?</p><p>7. Do you expect Lugol’s Iodine to move into the bag? ______Why or why not?</p><p>How will you test this?</p><p>8. In Fig. 1.1 in the Lab #1 packet, identify the hypotonic solution</p><p>______; the hypertonic solution ______; and the net direction of initial water flow ______. </p><p>9. A dialysis bag contains a sucrose solution of 0.6M. The beaker into which it is placed has a concentration of 0.3M. Is the dialysis bag hyper or hypotonic to the beaker solution? ______. In which direction is the water expected to travel?</p><p>10. What concentration of sucrose solution would the beaker need to contain for there to be no net movement of water between the 0.6M bag and the beaker? ______</p><p>11. What are the two components that influence water potential?</p><p>12. If a potato core is placed in pure water, in which direction would you predict the net flow of water to travel (if any)? Why?</p><p>13. Discuss the difference between plant and animal cells when placed in hypotonic solutions.</p><p>14. How does the movement of water relate to pressure? Give an example to illustrate this.</p><p>15. Increasing pressure increases or decreases the water potential number (circle one). 16. Increasing solute concentration increases or decreases the water potential number (circle one). </p><p>17. Circle the correct phrase: </p><p>Water will go from an area of -3 water potential to 0.</p><p>Water will go from an area of 0 water potential to -3.</p><p>18. If you continue to add solute to our potato cells what will happen to them?</p><p>19. Define plasmolysis.</p><p>20. Explain what you would expect to happen if you put salt on watermelon (very yummy). What about salt on slugs in your garden (very cruel).</p>
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