Macronutrient Composition of Semi-Synthetic High-Fat Diets (In % of Diet)

Macronutrient Composition of Semi-Synthetic High-Fat Diets (In % of Diet)

<p>Friedrich et al.: Supplement tables</p><p>TABLE S1</p><p>Macronutrient composition of semi-synthetic high-fat diets (in % of diet)</p><p>HF diet 13C-HF diet Ingredients (Experiment 1) (Experiment 2) Casein 18 20 Wheat starch 43 40 Saccharose 5 5 Palm kernel fat 18 - Linseed oil 1 - Safflower oil 1 - Corn oil - 17 Cellulose 7 11 Mineral mixture 5 5 Vitamin mixture 2 2 Energy content (kJ/g) 21.3 19.9</p><p>1 TABLE S2 Sequences of used primer-probe-pairs for investigation of gene expression through quantitative real-time PCR</p><p>Gene description/ Sequences (3´ 5´) Accession No. 18SrRNA f acc aca tcc aag gaa ggc ag NR_003278 r ttt tcg tca cta cct ccc c p 6-FAM - agg cgc gca aat tac cca ctc cc - TAMRA ACC f ttt cac tgt ggc ttc tcc ag NM_133360 r tgc att tca ctg ctg caa ta p 6-FAM - cca ttg ttg gcg ata aga acc ttc tca - TAMRA CD36/FAT f cca agc tat tgc gac atg at NM_007643 r ca gcg tag ata gac ctg caa a p 6-FAM - tgg cac aga cgc agc ctc ct - TAMRA Fabp2 f gtt gtg ttt gag ctc ggt gt NM_007980 r agc aat cag ctc ctt tcc at p 6-FAM-tcc ctc aat ggt cca ggc cc -TAMRA FAS f ttg atg att cag gga gtg ga NM_007988 r tta cac ctt gct cct tgc tg p 6-FAM - cat aga ccc gcc gag cca gg - TAMRA FATP4 f ttc atc aag acg gtc agg ag NM_011989 r acc att gaa gca aac agc ag p 6-FAM - tca gga gca cca tgc cac ca - TAMRA Glut2 f atc cag gcg aat tta tcc ag NM_031195 r ctg ttc cta acc ggg atg at p 6-FAM - ccc acc gac atg aag atg gtg c - TAMRA SCD1 f ttc ttc tct cac gtg ggt tg NM_009127 r cgg gct tgt agt acc tcc tc p 6-FAM - cgc aaa cac ccg gct gtc aa - TAMRA SGLT1 f cgc cag tac tct ctt cac ca NM_019810 r acg agg atg aac aac ctt cc p 6-FAM - aca cca aga tcc gga aga agg ca -TAMRA</p><p>18SrRNA: ribosomal RNA, ACC: Acetyl-CoA-Carboxylase, CD36/FAT: Cluster of differentiation 36 / Fatty acid translocase, Fabp2: Fatty acid binding protein 2, FAS: Fatty acid synthase, FATP4: Fatty acid transport protein 4, Glut2: Glucose transporter 2, SCD1:</p><p>Stearoyl-CoA-Carboxylase, SGLT1: Sodium dependent glucose transporter 1 </p><p>2</p>

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