<p>Risk assessment of chemical, laboratory experiment or process</p><p>The risk assessment shall contain information about current chemicals, storage, handling, preparation, use, end products, possible intermediate products and waste handling. Personal security and external environment shall be taken into consideration.</p><p>Chemical/Laboratory experiment/Process</p><p>Development of catalytic amperometric biosensor for the detection of glucose. The aim if the exercise is to develop two electrochemical glucosensors by the combination of commercial screen-printed electrodes, chemical and electrochemical deposited of catalysts/mediators and enzymes. Procedure: Surface of screen-printed electrode will be pretreated electrochemically in a saturated solution of Na2CO3 and functionalized electrochemically with Prussian Blue or chemically with ferrocene carboxylic acid. Then, glucose oxidase aqueous solution and Nafion solution in isopropanol will be mixed, dropped on the surface of electrode and left to dry at 4 °C for 60 minutes. Amperometric detection of glucose with fabricated biosensor will be performed. </p><p>Chemicals:</p><p>Na2CO3 FeCl3 HCl K3[Fe(CN)6)] FcCOOH KCl H2O2 glucose glucose oxidase Nafion isopropanol PBS –Na2HPO4, KH2PO4, NaCl and KCl</p><p>Area/Unit Biosensors and Bioelectronics Laboratory/Premises H302 Risk assessment</p><p>X Low risk Moderately hazardous Hazardous Very hazardous</p><p>Inherent chemicals intrinsic hazardous properties (also end products and intermediate products if possible) Na2CO3</p><p>Causes serious eye irritation.</p><p>FcCOOH</p><p>Irritant in contact with skin and eyes </p><p>HCl Hazardous in case of skin contact (corrosive, irritant, permeator), of eye contact (irritant, corrosive), or ingestion. Slightly hazardous in case of inhalation (lung sensitizer). Thus, safety measures need to be used, please see below.</p><p>H2O2 Does not show hazardous properties at used concentration isopropanol Highly flammable. Irritating to eyes. Vapors may cause drowsiness and dizziness.</p><p>Prussian blue May cause eye, skin and respiratory irritation. May cause gastrointestinal irritation. Prolonged skin contact may result in dermatitis in sensitive individuals.</p><p>Risks with storage, preparation, handling and waste handling shall be described at normal conditions and regarding unexpected incidents. All chemical when not in use will be stored (after having been properly labelled) in ventilated cabinet. During storage mixing between reactive solutions will be minimized.</p><p>Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or repeated exposure. Always use adequate protection (see below)</p><p>Measures to limit risks (safety equipment, handling instructions, etc.) The worker should use laboratory gloves, lab coat and tightly fit protective eyes glasses. Personal precautions, protective equipment and emergency procedures Ensure adequate ventilation. Evacuate personnel to safe areas. Environmental precautions Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into the environment must be avoided. Methods and materials for containment and cleaning up Soak up with inert absorbent material and dispose of as hazardous waste. Keep in suitable, closed containers for disposal.</p><p>First aid measures and measures in case of fire and waste After inhalation If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen. After skin contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. Call a physician. After eye contact In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. After ingestion If swallowed, wash out mouth with water provided</p><p>Always report accident or a situation that could have led to an accident to the demonstrators.</p><p>Risk assessment performed by: Date Valerio Beni 2015/01/29 Employers’ signature Date</p>