<p> Table S1: List of isolates used in this study and their related information: location, source of isolation (CLIN: clinical, VET: veterinary and ENV: environmental), specific source and date of isolation. Mating types, allele numbers for the seven MLST loci and the corresponding sequence type (ST) are also presented.</p><p>Other collection Source of Mating GPD LAC SOD URA WM number Specific location Specific source Isolation date CAP59 PLB1 IGS1 ST number isolation type 1 1 1 5 a Eucalyptus tereticornis, i l Australia, NSW, WM 1008 ENV association with frass of a 2000 α 2 6 4 2 15 2 10 7 a</p><p> r Blacktown t moth larva s Australia, NSW, u WM 437 New8; H 18 CLIN Skin 1994 α 2 6 4 2 15 2 10 7 Newcastle A VPB 571-015; Australia, NSW, WM 198 VET Cat 1990 α 2 6 4 2 15 2 10 7 McBride Sydney, Camden Australia, NSW, Cat (15 years old), lymph WM 08.245 571 270 VET 1996 α 2 6 4 2 15 2 10 7 Sydney node 49435; CBS 10082; Australia, NSW, **,¶ CLIN Lung 1991 α 1 17 16 14 19 7 16 21 WM 178 IFM 50894 Sydney Australia, NSW, ‡,¶ 571116; V20 VET Dog, abdominal mass 1995 α 2 6 4 2 15 2 10 7 WM 05.230 Sydney, Bradbury VPB 571-058; PR563; Australia, NSW, †,‡,¶ VET Cat (Birman cat), nasal 1991 α 2 6 4 2 15 2 10 7 WM 04.71 V4; CP 2500 / 91 Sydney, Chiswick Australia, NSW, †,‡,¶ C 194; V9 VET Cat (Pusska Shepherd) 1992 α 2 6 4 2 15 2 10 7 WM 08.284 Sydney, Coogee Australia, NSW, WM 08.267 571 327 VET Cat, nasal 2006 α 2 6 4 2 15 2 10 7 Sydney, Gladesville Australia, NSW, WM 2209 1607/96 VET Cat (3.5 years old), nasal 1996 α 2 6 4 2 15 2 10 7 Sydney, Rosemeadow Australia, NT, †,‡,¶ RDH06 CLIN CSF 1996 α 2 6 4 2 15 2 10 7 WM 04.70 Arnhemland Australia, NT, **,†,‡,¶ NT-08 CLIN Lung 1983 α 3 2 7 1 18 5 6 33 WM 06.1 Arnhemland Australia, NT, †,‡ H 16 CLIN Lung 1996 α 3 2 7 1 18 5 6 33 WM 2606 Arnhemland Australia, NT, †,‡,¶ NT-07 CLIN CSF 1989 α 3 2 7 1 18 5 6 33 WM 3030 Arnhemland Australia, NT, Eucalyptus tree (near ¶ ARN 001 ENV 21/06/1999 α 2 6 4 2 15 2 10 7 WM 03.456 Arnhemland, house) Australia, NT, **,†,‡,¶ NT-13 CLIN Lung 1985 α 2 6 4 2 15 2 10 7 WM 05.229 Arnhemland Australia, NT, ¶ RAM 002 ENV Eucalyptus tree 22/06/1999 α 2 6 4 2 15 2 10 7 WM 03.27 Arnhemland Australia, NT, ‡,¶ RAM 005 ENV E. tetrodonta 22/06/1999 α 2 6 4 2 15 2 10 7 WM 04.187 Arnhemland Australia, NT, E. miniata (100 m north of ‡,¶ RAM 015 ENV 22/06/1999 α 2 6 4 2 15 2 10 7 WM 04.69 Arnhemland oval) †,‡ WM 3032 NT-14 Australia, NT, CLIN CSF 1983 α 3 2 7 1 18 5 6 33 Katherine Region Australia, QLD, WM 09.4 37098-9217 CLIN Male (50 years old) 2008 α 2 6 4 2 15 2 10 7 Aurukun WM 08.141 571156 Australia, QLD, Maleny VET Cat, nasal 1996 α 2 6 4 2 15 2 10 7 PWQ 875, 5018; Q99; WM 08.306 Australia, WA VET Horse (name RIjInski ) 15/12/1999 α 2 6 4 2 15 2 10 7 86773K PWQ 606, 14-1419; Horse (name: Olivieri; 5 WM 08.308; WM 2971 S00 3895-U; MA Australia, WA, Perth VET 12/07/1995 α 2 6 4 2 15 2 10 7 years old), tracheal wash 21832 PWQ 320; 91/684; Horse (name: Grey all WM 09.85 Australia, WA VET 06/02/1991 α 2 6 4 2 15 2 10 7 91/183; noble), tracheal wash PWQ 321; 88/1337; Horse (cough after exercise, WM 09.86 Australia, WA VET 08/03/1988 α 2 6 4 2 15 2 10 7 88/460 lung lesion) Australia, WA, near WM 09.88 PWQ 494; 93/3258 VET Sheep L2 (horn lesion) 04/06/1993 α 2 6 4 2 15 2 10 7 Busselton Australia, WA. near WM 09.89 PWQ 496; 93/3257 VET Sheep L1 (eye lesion) 04/06/1993 α 2 6 4 2 15 2 10 7 Busselton PWQ 474; P93/133; Australia, WA, near WM 09.90; WM 08.307 VET Sheep 093-133, cid (tumor) 20/01/1993 α 2 6 4 2 15 2 10 7 93/355; 14-1408 Busselton Horse (name: Gavin "Kiwi WM 09.91 PWQ 628; 95/3662 Australia, WA VET 24/05/1995 α 2 6 4 2 15 2 10 7 Habit") tracheal wash PWQ 1781; WM 09.98 Australia, WA VET Dog, cid, nasal swab 16/12/2008 α 2 6 4 2 15 2 10 7 P08/2061902G PWQ 1009; Australia, WA, Dog, cid, cerebrospinal fluid WM 09.94 VET 21/12/2001 a 14 21 28 27 46 2 27 38 Q01/1067864N Geraldton area (3 years old) PWQ 730, 14-1431; Horse (name: Solidago), WM 08.309; WM 447 SOLIDAGO; M31557; Australia, WA VET 04/04/1997 α 27 6 4 1 43 7 4 48 lung M29004 PWQ 655, 14-1433; Horse (name: Malley Natasi WM 08.310 Australia, WA VET 29/11/1995 α 27 6 4 1 43 7 4 48 Malley; M25183 Aztec) PWQ 1675; Australia, WA, Cow # 1004 (tumor-like WM 09.97 VET 11/03/2008 α 2 6 4 2 15 2 10 7 P08/2030588F Busselton, Alton Park lesions through the lungs) Australia, WA, Eucalyptus log under Koala, WM 09.153 - Caversham Wildlife ENV 08/07/2009 α 2 6 4 2 15 2 10 7 new shed Park Australia, WA, WM 09.154 - Caversham Wildlife ENV Eucalyptus log, new shed 08/07/2009 α 2 6 4 2 15 2 10 7 Park Australia, WA, Eucalyptus tree trunk I, old WM 09.163 - Caversham Wildlife ENV 08/07/2009 α 2 6 4 2 15 2 10 7 shed Park Australia, WA, Vertical Eucalyptus log, old WM 09.164 - Caversham Wildlife ENV 08/07/2009 α 2 6 4 2 15 2 10 7 shed Park Australia, WA, Vertical Eucalyptus log WM 09.166 - Caversham Wildlife ENV 08/07/2009 α 2 6 4 2 15 2 10 7 (second), visitor area Park WM 09.155 - Australia, WA, VET Koala (name: Sarah), nasal 08/07/2009 α 2 6 4 2 15 2 10 7 Caversham Wildlife swab right nose●, new shed Park Australia, WA, Koala (name: J), nasal swab WM 09.156 - Caversham Wildlife VET 08/07/2009 α 2 6 4 2 15 2 10 7 ●●, Park right nose new shed Australia, WA, Koala (name: Amie), nasal WM 09.157 - Caversham Wildlife VET 08/07/2009 α 2 6 4 2 15 2 10 7 ●, Park swab right nose new shed Australia, WA, Koala (name: Amelie), nasal WM 09.158 - Caversham Wildlife VET 08/07/2009 α 2 6 4 2 15 2 10 7 ●, Park swab left nose new shed Australia, WA, Koala (adult male), nasal WM 09.160 - Caversham Wildlife VET swab left and right nose●, 08/07/2009 α 2 6 4 2 15 2 10 7 Park new shed Australia, WA, Koala (no name, female), WM 09.161 - Caversham Wildlife VET nasal swab left and right 08/07/2009 α 2 6 4 2 15 2 10 7 Park nose●, new shed Australia, WA, Eucalyptus tree trunk II, old WM 09.165 - Caversham Wildlife ENV 08/07/2009 a 14 21 28 27 46 2 27 38 shed Park Australia, WA, Eucalyptus log above WM 09.152 - Caversham Wildlife ENV 08/07/2009 α 27 6 4 1 43 7 4 48 Eucalyptus trunk, new shed Park Australia, WA, Male (54 years old) WM 09.83 PWQ 309 CLIN 17/12/1985 α 27 6 4 1 43 7 4 48 Mandurah cerebrospinal fluid Australia, WA, Female (24 years old) WM 09.95 PWQ 1040 CLIN 26/08/2002 α 27 6 4 1 43 7 4 48 Meekatharra Sputum Australia, WA, Middle Male (40 years old) WM 09.96 PWQ 1671 CLIN 29/01/2008 α 27 6 4 1 43 7 4 48 Swan cerebrospinal fluid PWQ 697, 14-1434; WM 08.311; WM Dog (Dalmatian), cranial Jackson; 6003508t; Australia, WA, Perth VET 10/04/1996 α 3 16 4 9 23 2 15 5 *,**,¶ mediastinal mass 08.131; WM 2517 571140; WA861 Australia, WA, Perth, Male (40 years old), WM 1382 PWQ 777 CLIN 18/03/1998 α 2 6 4 2 15 2 10 7 Willetton cerebrospinal fluid WM 04.72 PWQ 314; W12 Australia, WA, Perth CLIN Male (49 years old) 31/07/1990 α 3 2 7 1 18 5 6 33</p><p> a Canada, British c *,**,‡‡ 20 i CDC R265 Columbia, Vancouver CLIN Male, bronchial wash 2001 α 1 1 4 1 14 7 4 r WM 02.32 (=VGIIa) e Island, Duncan</p><p> m Canada, British WM 06.25; WM 7 A *,**,‡‡ CDC R272 Columbia, Vancouver CLIN Female, bronchial wash 2001 α 2 6 4 2 15 2 10 02.39 (=VGIIb) h Island, Ladysmith t r 6</p><p> o - B7432 USA, Oregon CLIN Blood 05/01/2009 - 4 6 4 1 15 2 15 (=VGIIc) N ATCC 32609. USA, Maryland, ¶,** CLIN Sputum 1975 α 1 1 4 1 14 7 4 20 WM 06.4 NIH 444. CBS 6956 Bethesda CBS 7750 ; USA, California, San E. camaldulensis (bark ¶,** ENV 1990 α 1 1 4 1 14 7 4 20 WM 06.13 451-SF13.2 Francisco debris) WM 02.35 CDC R268 Canada, British CLIN Female, cerebrospinal fluid 2001 α 1 1 4 1 14 7 4 20 Columbia, Lantzville CBS11511; Female (53 years old), </p><p> e WM 09.113 Switzerland CLIN 2006 α 1 1 4 1 14 7 4 20 RKI06/496 cerebrospinal fluid p</p><p> o CBS10485; Male (51 years old), lung </p><p> r WM 09.144 Denmark, Herning CLIN 2006 α 1 1 4 1 14 7 4 20 E758/2005 biopsy u</p><p>E WM 05.77** AV55 ; CBS 10090 Greece, Athens CLIN Female (26 years old) 1998 a 2 27 4 1 8 7 10 18 Republic of Korea, Male (57 years old), - K71 CLIN 2006 α 2 6 4 2 15 2 10 7 Asia Gyeonggi cerebrospinal fluid ¶,** WM 06.33 CBS 1930 ; RV 71421 Aruba VET Goat 1953 a 2 6 4 18 12 10 25 25 Male (18 years old), </p><p> a WM 05.452** LMM 417 ; LA 55 Brazil, Piaui CLIN 1995 a 2 27 4 1 8 7 15 16 cerebrospinal fluid c i r WM 05.470 LMM 547.18.13A3 Brazil, Piaui ENV Wood 1994 α 2 16 4 26 15 2 42 26 e LA 43 ; NUAM 176; Uruguay, Quebrada de Nest of wasp Polybia </p><p> m ¶,** WM 06.8 ENV 1996 α 7 6 21 25 2 2 32 3</p><p>A CBS 8684 los Cuervos occidentalis Brazil, Mato Grosso do Female (18 years old), h</p><p> t WM 05.533 LMM 892 CLIN 1997 α 10 6 30 16 20 2 16 11 Sul cerebrospinal fluid u</p><p> o LA 84 ; HEC 11102 ; WM 477 Brazil, Rio de Janeiro CLIN Male (47 years old), urine 1994 α 28 6 29 18 1 1 15 4 S LMM 21 Male (33 years old), WM 05.529 LMM 855 Brazil, Roraima CLIN 1997 α 2 32 30 1 13 7 25 27 cerebrospinal fluid Isolates used in other population genetic studies: † [25]; ‡ [17]; ‡‡ [27]; ¶ [16]; * [29]; ** [30]; ● Animals were colonized or had subclinical infections; ●● Koala with an infection of the right claw treated with Fluconazole daily and Amphotericin B 2x weekly.</p>