<p>ELECTRONIC SUPPLEMENTARY MATERIAL</p><p>SEDIMENTS, SEC 1 • SEDIMENT QUALITY AND IMPACT ASSESSMENT • RESEARCH ARTICLE</p><p>Assessing metal bioaccumulation from estuarine sediments: comparative experimental results for the polychaete Arenicola marina</p><p>M. Carmen Casado-Martinez • Brian D. Smith • Philip S. Rainbow</p><p>Received: 25 November 2011 / Accepted: 19 September 2012</p><p>© Springer-Verlag 2012</p><p>Responsible editor: Wolfgang Ahlf</p><p>M. C. Casado-Martinez () • B. D. Smith • P. S. Rainbow Department of Zoology, The Natural History Museum, Cromwell Rd, London SW7 5BD, United Kingdom</p><p>M. C. Casado-Martinez () Ecotox Centre, Swiss Center for Applied Ecotoxicology, Eawag/EPFL, EPFL-ENAC-IIE-GE Station 2 (GR B0 391), 1015 Lausanne, Switzerland e-mail: [email protected]</p><p>() Corresponding author: M. C. Casado Martinez Tel.: +41 (0)21 693 08 96 Fax.: +41 (0)21 693 80 35 e-mail: [email protected] Table S1 Biota-sediment accumulation factors (BSAF) calculated as the ratio between the concentrations measured in worms and the corresponding concentration measured in the exposure sediment derived from 30 d laboratory exposures and field collected individuals Thames estuary Dulas Bay Gannel estuary Laboratory Field Laboratory Field Laboratory Field Ag 0.95 0.93 7.1 2.0 2.8 1.1 As 4.3 4.5 1.8 0.60 1.1 2.8 Cd 0.47 0.78 1.2 3.9 0.40 0.43 Cu 0.62 0.67 0.28 0.43 0.58 0.58 Pb 0.05 0.07 0.08 0.04 0.02 0.11 Zn 0.97 0.98 0.34 0.15 0.33 0.49 Fig. S1 Bioaccumulated concentrations (μg g-1) measured in individuals of Arenicola marina from laboratory exposures to the Thames sediments (×), and model predicted concentrations in the absence of sediment ingestion (▲) and with an optimal IR as calculated following Cammen, 1980 (■)</p><p>0.6 0.9 m m r r o o w w 0.4 </p><p> n n i 0.6 i</p><p> n n c c n n o</p><p> o 0.2 c c 0.3</p><p> d g C A 0 0 0 10 20 30 0 10 20 30</p><p>90 45 m r m r o o w w</p><p>30 60 n i n i</p><p> c n n c o n c</p><p> o 15</p><p>30 s c</p><p>A n Z 0 0 0 10 20 30 0 10 20 30 Time (d) Time (d)</p><p>Fig. S2 Bioaccumulated concentrations (μg g-1) measured in individuals of Arenicola marina from laboratory exposures to the Gannel sediments (×), and model predicted concentrations in the absence of sediment ingestion (▲) and with an optimal IR as calculated following Cammen, 1980 (■)</p><p>0.9 1.8 m m r r o o w w</p><p> n n</p><p> i 0.6 i 1.2</p><p> n n c c n n o o c c</p><p>0.3 0.6 g d A C</p><p>0 0 0 10 20 30 0 10 20 30</p><p>60 90 m m r r o o w w</p><p>40 n i 60 n i n n c c n n o o c</p><p>30 c 20 n s Z A</p><p>0 0 0 10 20 30 0 10 20 30</p><p>Time (d) Time (d) Fig. S3 Bioaccumulated concentrations (μg g-1) measured in individuals of Arenicola marina from laboratory exposures to the Dulas Bay sediments (×), and model predicted concentrations in the absence of sediment ingestion (▲) and with an optimal IR as calculated following Cammen, 1980 (■)</p><p>0.9 0.9 m m r r o o w w</p><p> n n i</p><p> i 0.6 0.6</p><p> n n c c n n o o c c</p><p>0.3 0.3 d g C A 0 0 0 10 20 30 0 10 20 30</p><p>75 90 m m r r o o w w</p><p>50 n n</p><p> i 60 i</p><p> n n c c n n</p><p> o o 25 c c</p><p>30 s n Z A</p><p>0 0 0 10 20 30 0 10 20 30</p><p>Time (d) Time (d) </p>