<p>UC Irvine Environmental Health & Safety: Example Standard Operating Procedures for Hazardous Chemicals and Particularly Hazardous Substances</p><p>Hazardous Chemicals #1 Process Handling ENU in animals. Please ensure employees read the Material Safety Data Sheet at: #2 Hazardous Chemicals/Class of Hazardous Chemicals N- Nitroso –N ethylurea (synonym ENU). Staff must review the Material Safety Data Sheet prior to working with any hazardous material. Protocol designed for liquid stock of ENU, not powder form. #3 PPE - Personal protective Equipment Use disposable nitrile gloves and a lab coat at all times when working with ENU. #4 Engineering/Ventilation Controls All procedures involving ENU must be handled inside the fumehood. Verify date of inspection of the fumehood on the sticker. Fumehood must have been inspected within the lst year. #5 Special Handling Procedures and Storage Requirements 1. In a chemical hood, measure the appropriate volume of ENU into a syringe. No handling of dry powders.</p><p>2. Keep mice inside fume hood for at least 24 hours after exposure to ENU. When removing mice from fume hood, transfer into clean cage and discard bedding as hazardous (solid) waste. The PI’s staff will change contaminated bedding and place dirty bedding into a doubled plastic bag containing paper saturated with inactivating solution. (see solution below), then sealed and placed in a rigid leak proof container labeled as hazardous waste. Procedure will be conducted inside a fume hood.</p><p>3. Subsequent bedding changes may be disposed of as non-hazardous bedding waste. #6 Spill and Accident Procedures 1. Clean all spills and soak all equipment, syringes and gloves coming into contact with ENU with the inactivating solution and discard in a rigid leak proof container labeled as hazardous waste. </p><p>2. Inactivate any remaining ENU on surfaces with 20-30 mls of inactivating solution. Let stand in the chemical hood exposed to light overnight and discard in a rigid leak proof container labeled as hazardous waste.</p><p>3. If nitrile gloves should become contaminated while working with ENU, apply inactivating solution on glove and remove immediately and wash hands with soap and water.</p><p>4. Inactivating Solution: 20% w/v Na2S2O3 in 0.1N NaOH. #7 Waste Disposal Dispose of any liquid thiosulfate in a rigid leak proof container labeled as hazardous waste. Contact EH&S for disposal as hazardous waste. (containers or pails may be obtained from physical sciences stores or a lab safety supply vendor). #8 Approval Required: Approval by PI or Lab Supervisor required to perform protocol. Reviewed by EH&S: S. Weekly, L. Cambria Campus Veterinarian G. Mulder #9 Decontamination Procedures Apply solution to all contaminated surfaces. See Item 6. Formula for Deactivating solution:</p><p>50 gm Na2S203 (Sodium Thiosulfate) 1 gm NaOH</p><p> up to 250 mls with dH20 #10 Designated Area Vivarium No. ______For more information relevant to completing this form, review thoroughly Chemical Hygiene Plan: Section 5. Training Acknowledgement: I have read, asked questions, and understand the hazards of and safe working procedures for the activity/materials described herein. NAME DATE</p><p>These safety training resources, prepared solely for the use of the Regents of the University of California, were provided by a variety of sources. It is your responsibility to customize the information to match your specific operations. Neither the University of California nor any of its employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would not infringe privately owned rights. Reference herein to UC Irvine Environmental Health & Safety: Example any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the University of California. The views and opinions of authors expressed herein do not necessarily state or reflect those of the University of California, and shall not be used for advertising or product endorsement purposes.</p>