<p> University of Wisconsin Oshkosh Institutional Biosafety Committee Sponsored Programs & Faculty Development</p><p>Laboratory Biological Safety Assessment Checklist </p><p>Lab Assessment Date: ______PI Name: ______</p><p>Protocol/Registration Number (s): ______</p><p>Location of Lab(s)/Workspace(s): ______</p><p>Research OR Teaching Space: ______</p><p>Individual(s) Conducting Lab Assessment: ______</p><p>Biosafety Level: ______</p><p>Overview of Research & Teaching Activity- PI Provides Summary of Work Conducted in Lab</p><p>Y N N/A Comments Do you work with human, non-human primate, or sheep derived source materials (e.g. blood, body fluid, tissue or cell lines)? If yes, have all staff completed blood borne pathogens training within the past year?</p><p>Do you use any materials derived from potentially infectious substances (e.g. purified protein, protein conjugated to Ab)?</p><p>Do you work with animal materials (e.g. blood, body fluids, tissue, or cell lines)? Do you handle potentially infectious animal materials? Do you work with infectious agents of human or animal origin? (e.g. virus, bacteria, fungi, protozoa, parasite, prion)?</p><p>Is recombinant work done in the lab (exempt or non-exempt)? Do you use biological toxins or toxin subunits (e.g. diphtheria toxin, cholera toxin, tetrodotoxin, conotoxins, pertussis toxin)?</p><p>Protocols, SOPs, and Training</p><p>Y N N/A Comments All work conducted in this lab is covered by an up-to-date IBC Protocol or Registration. PI has documentation of training provided to research personnel and/or students for classroom projects. Training is complete for all individuals listed on IBC Protocol or Registration. Training requirements may be found on IBC website. Research personnel have access to the IBC Protocol or Registration (i.e., located in shared lab binder, google drive, dropbox, etc) and are familiar with its contents. Laboratory-specific procedures are available to staff. Staff have read these documents. Do you have a biological inventory? (system for tracking biological materials, disposal, storage). Personnel are familiar with the IBC Incident Report Form and how to report spills, exposures, injuries, and illnesses to the IBC, including the reporting timeframe.</p><p>Aerosol Generating Activities Sponsored Programs & Faculty Development Institutional Biosafety Committee Updated: 9/7/17 University of Wisconsin Oshkosh Institutional Biosafety Committee Sponsored Programs & Faculty Development</p><p>Laboratory Biological Safety Assessment Checklist </p><p>Y N N/A Comments Do you generate biological aerosols during your work? What precautions are taken when using centrifuges? (e.g. buckets with Comments: biocontainment caps, screw cap tubes, open buckets and tubes only in BSC) What precautions are taken when using a sonicator or homogenizer? (e.g. Comments: use in BSC, use in sealed enclosure or tubes, towel with disinfectant over top) What other procedures could generate aerosols and how do you protect Comments: yourself? (e.g. using pipets/needles/syringes, mixing, vacuum system, transfer loops, opening tubes) Personal Protective Equipment (PPE)</p><p>Y N N/A Comments PPE is available and utilized by all persons entering the laboratory/workspace and is appropriate to the work being conducted. PPE in use matches what is listed in the protocol and/or registration. PPE is not worn in public hallways (the one gloved hand method may be utilized if transporting materials in the hallway) Employees voluntarily wearing respirators have been given OSHA- Appendix D- Employees Using Respirators When Not Required Under the Standard. Safety Equipment: Biosafety Cabinet, Laminar Flow Hoods, Fume Hoods </p><p>Y N N/A Comments Laminar Flow Hood/Clean Bench used for procedures Fume Hood is used in lab Lab uses Biosafety Cabinet (BSC) Type of BSC/Class: If the BSC is in use it has been certified within the past year. BSC certification label is on unit The BSC is kept free of clutter and the front grate is kept clear. Are you using heat-generating items in the BSC (i.e. Bunsen burner, propane cylinders, gas plumbed in the BSC)? Do you use the UV light in the BSC? The BSC vacuum lines are protected with liquid trap and in-line High Efficiency Particulate Air (HEPA) filters, as per http://www.cdc.gov/ biosafety/publications/bmbl5/index.htm, Section IV (pg. 49), BSL-2 requirements. Signage and Accessibility Y N N/A Comments Signage is present on door exterior for BSL-2 labs with placards signifying BSL, PPE required for entry, agent(s) or material(s) in use, and potential health effects. Emergency contact information is current and posted on the door exterior/ interior entranceway. All equipment used for biohazardous/biological materials is labeled with a Biohazard sticker.</p><p>Sponsored Programs & Faculty Development Institutional Biosafety Committee Updated: 9/7/17 University of Wisconsin Oshkosh Institutional Biosafety Committee Sponsored Programs & Faculty Development</p><p>Laboratory Biological Safety Assessment Checklist Access to the laboratory/workspace is limited or restricted while experiments are in progress. PI has final responsibility for determining who may enter or work in the space. General Facility Condition Y N N/A Comments Sharps are safely used and disposed of properly. No recapping of needles. Sharps containers disposed when they reach 2/3-3/4 full. Sharps containers are sealed and delivered to Biology Stockroom when ready for disposal. All biological wastes are decontaminated before disposal, and follow the procedure listed in IBC Protocol. Laboratory clutter is minimized and storage of cardboard is minimized. All surfaces and furniture in the lab work area are impermeable. Food and drink are not stored or consumed in the lab. Sign is posted for No Food or Drink. Biological materials in the lab are appropriately labeled. Hand washing supplies are readily available within the lab (i.e. sink, soap, towels, trash can). A biological spill kit is available and readily accessible. Kit is appropriate for the materials used in lab. Kits contain instructions for handling spill. Transport of hazardous materials between rooms and buildings is conducted in an appropriate manner (e.g. secondary containment, appropriately labeled). Plumbed emergency shower and eyewash (if required), are available and unobstructed (sign for emergency shower and eyewash is clearly visible). Eyewash is flushed weekly and documented by Facilities Management. If eyewash bottles are present in the lab, they are full and not out of date. Exits and corridors free from obstructions (i.e., lab aisle space are open at least 36-inches and hallways/corridors are open at least 44-inches for traffic).Doors are kept closed in order to maintain proper room pressure. Biological Waste Handling Y N N/A Comments Is Regulated Medical Waste (RMW or infectious waste) being generated? If so, is it stored in a rigid closed container and sterilized prior to disposal? Lab generates rDNA (BSL1 or 2) or BSL1 biological liquid/solid waste (e.g. nonpathogenic bacteria used in cloning, animal-derived materials). If so, is it stored in a rigid closed container with a clear autoclave bag and treated prior to disposal? Biological waste is properly segregated (autoclave, chemical treatment or incineration). Non-regulated sharp waste is decontaminated and disposed as general glass waste (broken or unbroken glass, glass vials, laboratory slides, Pasteur pipettes). Autoclave waste is separate from sharps waste (i.e. solid waste that is not a regulated sharp such as PPE, plastic tips, and vacutainers). Regulated sharps are generated (e.g. hypodermic needles, syringes with needles, scalpel blades, razor blades, lancets, infectious glass, rigid plastic vials or slide) and are properly disposed (puncture resistant container, no autoclaving & EHS waste pickup for off-site disposal). Lab has safe sharps practices. PPE is used when handling sharps. </p><p>Sponsored Programs & Faculty Development Institutional Biosafety Committee Updated: 9/7/17</p>