<p> Journal: Molecular and Cellular Biochemistry</p><p>Analysis of human bitter taste receptors expression in extraoral tissues</p><p>Appalaraju Jaggupillia,b, Nisha Singha,b, Jasbir Upadhyayaa,b, Anurag S. Sikarwara,b, Makoto Arakawaa,b,c, </p><p>Shyamala Dakshinamurtia,d,e, Rajinder P. Bhullara,b, Kangmin Duana,b,e, and Prashen Chelikania,b,e,f*</p><p> aManitoba Chemosensory Biology (MCSB) Research Group, bDepartment of Oral Biology, University of </p><p>Manitoba, Winnipeg, Canada, MB, R3E 0W4, cDepartment of Dental Hygiene, Chiba Prefectual </p><p>University Of Health Sciences, d Departments of Pediatrics, Physiology, University of Manitoba, eBiology of Breathing, Children’s Hospital Research Institute of Manitoba (CHRIM), f Department of </p><p>Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada, MB, R3E 0W4</p><p>* Correspondent Author: Dr. Prashen Chelikani, D319, Department of Oral Biology, and Manitoba </p><p>Chemosensory Biology (MCSB) Research Group, University of Manitoba, Winnipeg, MB R3E 0W4. </p><p>Canada 204-789-3539 (phone), 204-789-3913 (fax), email: [email protected] Journal: Molecular and Cellular Biochemistry TAS2R45 ATGATAACTTTTCTGCCCATCATATTTTCCATTCTAGTAGTGGTTACATTTGTTATTGGAAATTTTGCTAATGGCTTCATAGCGTTGGTAAATTCCACCGAGTGGGTGAAGAGACAAAAGATCTCCTTT TAS2R30 ATGATAACTTTTCTGCCCATCATTTTTTCCATTCTAATAGTGGTTATATTTGTTATTGGAAATTTTGCTAATGGCTTCATAGCATTGGTAAATTCCATTGAGTGGGTCAAGAGACAAAAGATCTCCTTT TAS2R46 ATGATAACTTTTCTGCCCATCATTTTTTCCATTCTAATAGTGGTTACATTTGTGATTGGAAATTTTGCTAATGGCTTCATAGCATTGGTAAATTCCATTGAGTGGTTCAAGAGACAAAAGATCTCTTTT TAS2R31 ATGACAACTTTTATACCCATCATTTTTTCCAGTGTGGTAGTGGTTCTATTTGTTATTGGAAATTTTGCTAATGGCTTCATAGCATTGGTAAATTCCATTGAGCGGGTCAAGAGACAAAAGATCTCTTTT TAS2R43 ATGATAACTTTTCTACCCATCATTTTTTCCAGTCTGGTAGTGGTTACATTTGTTATTGGAAATTTTGCTAATGGCTTCATAGCACTGGTAAATTCCATTGAGTGGTTCAAGAGACAAAAGATCTCCTTT **** ******* * ******** ******* * * ******** ****** ***************************** ************ *** ** * ***************** ***</p><p>TAS2R45 GCTGACCAAATTGTCACTGCTCTGGCGGTCTCCAGAGTTGGTTTGCTCTGGGTGTTATTATTAAATTGGTATTCAACTGTGTTGAATCCAGCTTTTTATAGTGTAGAATTAAGAACTACTGCTTATAAT TAS2R30 GTTGACCAAATTCTCACTGCTCTGGCGGTCTCCAGAGTTGGTTTGCTCTGGGTGTTATTACTACATTGGTATGCAACTCAGTTGAATCCAGCTTTTTATAGTGTAGAAGTAAGAATTACTGCTTATAAT TAS2R46 GCTGACCAAATTCTCACTGCTCTGGCAGTCTCCAGAGTTGGTTTACTCTGGGTATTAGTATTAAATTGGTATGCAACTGAGTTGAATCCAGCTTTTAACAGTATAGAAGTAAGAATTACTGCTTACAAT TAS2R31 GCTGACCAGATTCTCACTGCTCTGGCGGTCTCCAGAGTTGGTTTGCTCTGGGTATTATTATTAAATTGGTATTCAACTGTGTTTAATCCAGCTTTTTATAGTGTAGAAGTAAGAACTACTGCTTATAAT TAS2R43 GCTGACCAAATTCTCACTGCTCTGGCGGTCTCCAGAGTTGGTTTGCTCTGGGTATTATTATTAAACTGGTATTCAACTGTGTTGAATCCAGCTTTTAATAGTGTAGAAGTAAGAACTACTGCTTATAAT * ****** *** ************* ***************** ******** *** ** ** * ****** ***** *** ************ * *** ***** ****** ********* ***</p><p>TAS2R45 ATCTGGGCAGTAACCGGCCATTTCAGCAACTGGCCTGCTACTAGCCTCAGCATATTTTATTTGCTCAAGATTGCCAATTTCTCCAACCTTATTTTTCTTCGCTTAAAGAGGAGAGTTAAGAGTGTCATT TAS2R30 GTCTGGGCAGTAACCAACCATTTCAGCAGCTGGCTTGCTACTAGCCTCAGCATGTTTTATTTGCTCAGGATTGCCAATTTCTCCAACCTTATTTTTCTTCGCATAAAGAGGAGAGTTAAGAGTGTTGTT TAS2R46 GTCTGGGCAGTAATCAACCATTTCAGCAACTGGCTTGCTACTAGCCTCAGCATATTTTATTTGCTCAAGATTGCCAATTTCTCCAACCTTATTTTTCTTCACTTAAAGAGGAGAGTTAAGAGTGTTGTT TAS2R31 GTCTGGGCAGTAACCGGCCATTTCAGCAACTGGCTTGCTACTAGCCTCAGCATATTTTATTTGCTCAAGATTGCCAATTTCTCCAACCTTATTTTTCTTCACTTAAAGAGGAGAGTTAAGAGTGTCATT TAS2R43 ATCTGGGCAGTGATCAACCATTTCAGCAACTGGCTTGCTACTACCCTCAGCATATTTTATTTGCTCAAGATTGCCAATTTCTCCAACTTTATTTTTCTTCACTTAAAGAGGAGAGTTAAGAGTGTCATT ********** * * *********** ***** ******** ********* ************* ******************* ************ * ********************** **</p><p>TAS2R45 CTGGTGGTGCTGTTGGGGCCTTTGCTATTTTTGGCTTGTCATCTTTTTGTGGTAAACATGAATCAGATTGTATGGACAAAAGAATATGAAGGAAACATGACTTGGAAGATCAAATTGAGGCGTGCAATG TAS2R30 CTGGTGATACTGTTGGGGCCTTTGCTATTTTTGGTTTGTCATCTTTTTGTGATAAACATGGATGAGACTGTATGGACAAAAGAATATGAAGGAAACGTGACTTGGAAGATCAAATTGAGGAGTGCAATG TAS2R46 CTGGTGATACTATTGGGGCCTTTGCTATTTTTGGTTTGTCATCTTTTTGTGATAAACATGAATCAGATTATATGGACAAAAGAATATGAAGGAAACATGACTTGGAAGATCAAACTGAGGAGTGCAATG TAS2R31 CTGGTGATGCTGTTGGGGCCTTTACTATTTTTGGCTTGTCAACTTTTTGTGATAAACATGAAAGAGATTGTACGGACAAAAGAATATGAAGGAAACTTGACTTGGAAGATCAAATTGAGGAGTGCAGTG TAS2R43 CTGGTGATGTTGTTGGGGCCTTTGCTATTTTTGGCTTGTCATCTTTTTGTGATAAACATGAATGAGATTGTGCGGACAAAAGAATTTGAAGGAAACATGACTTGGAAGATCAAATTGAAGAGTGCAATG ****** * * *********** ********** ****** ********* ******** * *** * * ************ ********** ***************** *** * ***** **</p><p>TAS2R45 TACCTTTCAGATACGACTGTAACCATGCTAGCAAACTTAGTACCCTTTACTGTAACCCTGATATCTTTTCTGCTGTTAGTCTGTTCTCTGTGTAAACATCTCAAGAAGATGCAGCTCCATGGCAAAGGA TAS2R30 TACCATTCAAATATGACTCTAACCATGCTAGCAAACTTTGTACCCCTCACTCTGACCCTGATATCTTTTCTGCTGTTAATCTGTTCTCTGTGTAAACATCTCAAGAAGATGCAGCTCCATGGCAAAGGA TAS2R46 TACCTTTCAAATACAACGGTAACCATCCTAGCAAACTTAGTTCCCTTCACTCTGACCCTGATATCTTTTCTGCTGTTAATCTGTTCTCTGTGTAAACATCTCAAAAAGATGCAGCTCCATGGCAAAGGA TAS2R31 TACCTTTCAGATGCGACTGTAACCACGCTAGGAAACTTAGTGCCCTTCACTCTGACCCTGCTATGTTTTTTGCTGTTAATCTGTTCTCTGTGTAAACATCTCAAGAAGATGCAGCTCCATGGTAAAGGA TAS2R43 TACTTTTCAAATATGACTGTAACCATGGTAGCAAACTTAGTACCCTTCACTCTGACCCTACTATCTTTTATGCTGTTAATCTGTTCTTTGTGTAAACATCTCAAGAAGATGCAGCTCCATGGTAAAGGA *** **** ** ** ****** *** ****** ** *** * *** * ***** *** **** ******** ******** **************** ***************** ******</p><p>TAS2R45 TCTCAAGATCCCAGTACCAAGGTCCACATAAAAGTTTTGCAAACTGTGATCTCCTTCTTCTTGTTACGTGCCATTTACTTTGTGTCTGTAATAATATCAGTTTGGAGTTTTAAGAATCTGGAAAACAAA TAS2R30 TCTCAAGATCCCAGCACCAAGGTCCACATAAAAGCTTTGCAAACTGTGACCTCCTTTCTTCTGTTATGTGCCATTTACTTTCTGTCCATGATCATATCAGTTTGTAATTTTGGGAGGCTGGAAAAGCAA TAS2R46 TCTCAAGATCCCAGCATGAAGGTCCACATAAAAGCTTTGCAAACTGTGACCTCCTTCCTCTTGTTATGTGCCATTTACTTTCTGTCCATAATCATGTCAGTTTGGAGTTTTGAGAGTCTGGAAAACAAA TAS2R31 TCTCAAGATCCCAGCACCAAGGTCCACATAAAAGCTTTGCAAACTGTGATCTTTTTCCTCTTGTTATGTGCCGTTTACTTTCTGTCCATAATGATATCAGTTTGGAGTTTTGGGAGTCTGGAAAACAAA TAS2R43 TCTCAAGATCCCAGCACCAAGGTCCACATAAAAGCTTTGCAAACTGTGATCTCCTTCCTCTTGTTATGTGCCATTTACTTTCTGTCCATAATGATATCAGTTTGGAGTTTTGGAAGTCTGGAAAACAAA ************** * **************** ************** ** ** * ***** ***** ******** **** * ** ** ******** * **** * ******** **</p><p>TAS2R45 CCTGTCTTCATGTTCTGCCAAGCTATTGGATTCAGCTGTTCTTCAGCCCACCCGTTCATCCTGATTTGGGGAAACAAGAAGCTAAAGCAGACTTATCTTTCAGTTTTGTGGCAAATGAGGTACTGA--- TAS2R30 CCTGTCTTCATGTTCTGCCAAGCTATTATATTCAGCTATCCTTCAACCCACCCATTCATCCTGATTTTGGGAAACAAGAAGCTAAAGCAGATTTTTCTTTCAGTTTTGCGGCATGTGAGGTACTGGGTG TAS2R46 CCTGTCTTCATGTTCTGCGAAGCTATTGCATTCAGCTATCCTTCAACCCACCCATTCATCCTGATTTGGGGAAACAAGAAGCTAAAGCAGACTTTTCTTTCAGTTTTGTGGCATGTGAGGTACTGGGTG TAS2R31 CCTGTCTTCATGTTCTGCAAAGCTATTAGATTCAGCTATCCTTCAATCCACCCATTCATCCTGATTTGGGGAAACAAGAAGCTAAAGCAGACTTTTCTTTCAGTTTTGCGGCAAGTGAGGTACTGGGTG TAS2R43 CCTGTCTTCATGTTCTGCAAAGCTATTAGATTCAGCTATCCTTCAATCCACCCATTCATCCTGATTTGGGGAAACAAGAAGCTAAAGCAGACTTTTCTTTCAGTTTTTTGGCAAATGAGGTACTGGGTG ****************** ******** ******** * ***** ****** ************* *********************** ** ************ **** ********** </p><p>TAS2R45 ------TAS2R30 AAAGACAGAAGCC-TTCGTCTCCATAGATTCACAAGAGGGGCATTGTGTGTCTTCTAG TAS2R46 AAAGGAGAGAAGCCTTCATCTTCATAG------TAS2R31 AAAGGAGAGAAGCCTTCATCTCCATAG------TAS2R43 AAAGGAGAGAAGACTTCATCTCCATAG------Journal: Molecular and Cellular Biochemistry Supplementary Figure 1. Multiple sequence alignment of human TAS2R30, 31, 43, 45 and 46. The gene sequences were retrieved from NCBI in the FASTA format and the multiple sequence alignment was carried out using Clustal Omega</p>