<p> Faculty of Health and Life Sciences</p><p>COSHH RISK ASSESSMENT FORM</p><p>Activity Title: Type of activity: Location of activity: Lactose practical 1 and 2 Undergraduate practical 1H4/5 or 2KH1</p><p>Assessed by (+date): Endorsed by (+date): Assessment review date: A. Ainger D Lush August 2015 August 2016</p><p>Description of experimental procedure. (Give sufficient detail so that it is clear as to the procedure being undertaken, to include substances to be used and importantly created)</p><p>Ortho-nitrophenyl-beta-D-Galactopyranoside (ONPG) is used instead of lactose as a substrate for the enzyme lactase. A yellow colour is produced which can be followed at 420nm in a spectrophotometer and record the data using a laptop.  Students prepare various concentrations of ONPG for session 1 to record initial rates of the reaction with a laptop attached to a spectrophotometer.  In session 2 students pipette various concentrations of galactose, which is used as an inhibitor of lactose. Students record initial rates of reaction with a laptop attached to a spectrophotometer  Students have an unknown galactose solution which they have to work out the concentration of.</p><p>Have you used the least hazardous substances possible in this procedure in order to eliminate or minimise the hazards to health? (✓)</p><p>Yes No (If No please justify )</p><p>Substance details Substance Quantity and Classification Hazard Statements Exposure (chemical, concentration (corrosive, toxic, to include exposure routes & potential Time (duration radiological or Amount in mL flammable, Biohazard health effects. / biological agents. or g then Group 1-4) From Label elements 2.2 – Hazard frequency of Including those concentration From pictogram 2.2 - Statements (H-numerical and use) created by in Mg/Ml, Please use words to description only). activity)Include %w/w, w/v, describe the pictogram. the common Molarity. See end of this form for chemical name description. from 1.1 0.1M TRIS/HCl 100mL bottle Not hazardous Not Hazardous Up to 2hrs pH 7 per 3 groups per session. (Tris(hydroxy 2 sessions methyl)amino methane) / hydrocholoric acid containing 20mM magnesium chloride</p><p>Page 1 of 3 April 2013 Lactase (β 1:20 dilution Not a hazardous This substance is not classified as Up to 2hrs galactosidase) 2mL bottle substance or mixture dangerous according to Directive per session. in per group according to 67/548/EEC. 2 sessions 0.1M Tris/HCl Regulation (EC) No. buffer pH 7 + 1272/2008. 20mM magnesium chloride o-Nitrophenyl 45 mM Not hazardous Not hazardous Up to 2hrs β-D- 20mL per per session. galactopyrano group 2 sessions side (ONPG) in 0.1M Tris/HCl buffer pH 7 containing 20mM magnesium chloride 0.666M 0.666M 10mL Not hazardous Not hazardous Up to 2hrs in Galactose in per group one session 0.1M Tris/HCl buffer pH 7 containing 20mM magnesium chloride Unknown 10mL per Not hazardous Not hazardous Up to 2hrs in concentration group one session. of Galactose in 0.1M Tris/HCl buffer pH 7 containing 20mM magnesium chloride Identify those persons at increased risk of harm (disabled, pregnancy, immuno-compromised etc) None</p><p>Control measures for the activity - from Precautionary Statements 2.2 i.e.: Engineering controls e.g. use of fume cupboard etc., PPE/RPE (detail type), restricting quantity of substance used, prohibiting lone working, level of supervision, exposure monitoring, relevant SOPs. </p><p>1. Induction of students into basic Health and Safety 2. Previous training of students in basic laboratory skills 3. A verbal introduction to the practical session will be delivered by a competent member of staff (academic and/or technical), during which specific risks and control measures will be highlighted to the students, including introduction of specific handling and dispensing techniques. 4. Use of personal protective equipment (PPE) – lab coat and disposable gloves. 5. Laboratory coat and nitrile gloves to be worn at all times.</p><p>Additional special measures of control i.e. particular training in handling the substances listed or for the experimental procedure, relevant health surveillance etc None</p><p>Page 2 of 3 April 2013 Indicate any specialist information relating to any of the substances in use e.g. storage, transportation, disposal, spillage, first aid Storage N/A Spillage If any of the solutions are spilled, clean up with a tissue and place in the normal waste bins. Disposal All liquids can be poured down the sink with plenty of water. First aid; N/A</p><p>Are any of the substances that you are using flammable, oxidising or explosive (name them)? No</p><p>Have you included suitable control measures above? Yes / No (delete as appropriate)</p><p>Page 3 of 3 April 2013</p>