<p>1</p><p>Supplemental Material</p><p>Table S1. Primer sequences for cloning, site-directed mutagenesis, and northern blot aThe base changes are shown in boldface and underlined. bfwd, forward; rev, reverse Name Sequence (5'/3') Directionb SF1 CACCATGCCGATGAGAATCGAGCGTG fwd SF2 TCACACATGAATTATTGCTTTCTCG rev SF4 ATGGTAGGTCTCATATCACACATGAATTATTGCTTTCTCGATG rev SF7 TCCGAATTCATGCCGATGAGAATCGAGCGTGATC fwd SF8 CGCAAGCTTTCACACATGAATTATTGCTTTCTC rev SK1 CACCATGTCCATAGCTGAAGCCTCAGC fwd SK2 CATCGAGAAAGCAATTCACCTGTGA rev SK12 TCCGAATTCATGAGAATAGAGCGTGATCTCCACATG fwd SK13 CGCAAGCTTTCACAGGTGAATTGCTTTCTCGATG rev OMT4-1 CACCATGAAGGTAGAGCGCGATTTCCAC fwd OMT4-2 TTATTTGGCGCGCCTCGCCTCAAGAG rev OMT5-1 CACCATGAATGTAGAGCGCGATCTCCAC fwd OMT5-2 TCATTTCGCCTGGAGGGAAACAAGG rev OMT6-1 CACCATGAAGGTAGAGCGCGATCTCCAC fwd OMT6-2 TCATTTCGCCTGGAGGGAAACGACG rev OMT7-1 ATGGTAGGTCTCAGCGCATGAATGTAGAGCGCGATCTCCA fwd OMT7-2 ATGGTAGGTCTCATATCATTTCGCCTGGAGGGAAACGACG rev OMT8-1 ATGGTACGTCTCAGCGCATGGATATAAGACGTGATTTCCATAT fwd OMT8-2 ATGGTACGTCTCATATCACTCTTTCTTCAAGCACATGGCGA rev</p><p>Mutation Sequence (5'/3')a Directionb Y246W AGAGTCCAACCATCTTTGGGGATTGCTTGCGCAGTC fwd Y246W GACTGCGCAAGCAATCCCCAAAGATGGTTGGACTCT rev Q167M CACTCTCTGTTCTGCCTTATGTGGCGCTCTCAGGCACC fwd Q167M GGTGCCTGAGAGCGCCACATAAGGCAGAACAGAGAGTG rev Q167H CTCTCTGTTCTGCCTTCATTGGCGCTCTCAGGCACCA fwd Q167H TGGTGCCTGAGAGCGCCAATGAAGGCAGAACAGAGAG rev 2</p><p>Table S2. Primer sequences for QPCR aRNA polymerase II large subunit, bputative adenine phosphoribosyltransferase </p><p>Sequence Product Sense/Antisense Primer (5'/3') Position Tm Definition Length AAMT1 220 CCGACTTAGGCTGCTCATCA 170 56.5 GCTGCATCTCCCTTGTGTGTC 389 58.8 AAMT2 242 GATGTTGTGCATAACAGTATTAGGAGC 945 58.9 ATGCCAAGCGAGATAGTGAGAAAC 1186 59,1 AAMT3 214 CCGACTTAGGCTGCTCATCG 170 57.6 GCCTCACCCTTGTGGTTTCTT 383 57.9 RPB1a 188 GCTGGATGATGAGAATTGGAGACC 2.508 59,1 GCTTGAGGTTCACAGGCATAGG 2.695 58,7 putative PT1Ab 162 AGGCGTTCCGTGACACCATC 380 59,8 CTGGCAACTTCTTCGGCTTCC 541 59,1 3</p><p>Figure S1 Comparison of the deduced amino acid sequences of AAMT1-Del1, AAMT1-Del2, AAMT2-Del1, AAMT3-Del1, OMT4, OMT5, OMT6, OMT7, and OMT8 with CbSAMT from Clarkia breweri (Ross et al., 1999) and AmBAMT from Antirrhinum majus (Dudareva et al., 2000). Amino acids identical in all proteins are indicated by black boxes. Amino acids involved in SAM-binding are marked with a plus (+) and amino acids involved in acid binding are marked with a bar (-). Amino acids discussed in this paper are labeled with a triangle (▼). For accession numbers of CbSAMT and AmBAMT see Figure 5. </p>