<p> Supplementary Material</p><p>Molecular Imaging and Biology</p><p>Feasibility of lactadherin-bearing clinically available microbubbles as ultrasound contrast agent for angiogenesis</p><p>Kentaro Otani and Kenichi Yamahara</p><p>Department of Regenerative Medicine and Tissue Engineering, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan</p><p>Reprint requests and correspondence to: [email protected] Supplementary Figure 1: Changes in bubble concentration during targeted bubble preparation. a) During the process of targeted bubble preparation, the concentration of bubbles was significantly decreased. Moreover, addition of lactadherin further decreased the concentration of Sonazoid bubbles. b) By incubating Sonazoid bubbles with lactadherin, the number of adherent bubbles to the micro-tube wall was obviously increased (yellow arrows). *P < 0.05 vs. Untreated Sonazoid, †P < 0.05 vs. Sonazoid-PBS.</p>
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