<p>MLAB 2432 Microbiology</p><p>Streptococcus, Enterococcus and other Catalase Negative Gram Positive Cocci Learning Objectives</p><p>1. Describe the following characteristics common to the streptococci and related organisms: a. Gram stain morphology b. Catalase production</p><p>2. Categorize the species of streptococci based on the following classification systems: a. Lancefield ( serological) b. Brown (hemolysis)</p><p>3. Examine the clinical, epidemiological and laboratory findings associated with the following infections: a. Group A Streptococcus ( Streptococcus pyogenes) i. Pharyngitis ii. Erysipelas iii. Impetigo iv. Scarlet fever v. Necrotizing fascitis vi. Toxic shock syndrome</p><p> b. Group B Streptococcus ( Streptococcus agalactiae) i. Neonatal pneumonia ii. Neonatal meningitis iii. Postpartum infections iv. UTI’s v. Osteomyelitis</p><p> c. Group D Streptococcus ( Streptococcus bovis) i. Bacteremia associated with colon malignancy</p><p> d. Enterococcus i. UTI’s ii. Wounds iii. Mixed infections of the pelvis and abdomen</p><p> e. Viridans Streptococcus group i. Subacute bacterial endocarditis (SBE)</p><p> f. Streptococcus pneumoniae MLAB 2432 Microbiology</p><p> i. Pneumonia ii. Meningitis iii. Otitis media iv. Sinusitis</p><p> g. Streptococcus anginosus/miliieri group i. Deep wound infections ii. Abdominal infections</p><p> h. Abiotrophia species ( nutritionally variant streptococci) i. Endocarditis</p><p>4. Examine the clinical and laboratory findings associated with the following post streptococcal sequelae: a. Acute glomerulonephritis b. Rheumatic fever</p><p>5. Explain the principle of the following biochemical tests, including the interpretation of results: a. Bacitracin/SXT susceptibility b. CAMP test c. Rapid hippurate hydrolysis d. Bile-esculin hydrolysis e. Growth in 6.5% NaCl f. Optochin susceptibility g. Bile solubility h. Latex agglutination i. PYR= Pyrrolidonyl arylamidase test j. Catalase production k. Staphylococcal streak plate l. Vancomycin susceptibility m. Rapid Strep tests</p><p>6. Describe the significance of the following virulence factors produced by Group A Streptococcus: a. Pyrogenic exotoxins b. Streptolysin O c. Streptolysin S d. Hyaluronidase e. DNase f. Streptokinase g. M protein MLAB 2432 Microbiology</p><p>7. Differentiate between the following Streptococcaceae based on the gram stain and colony morphology: a. Group A Streptococcus b. Group B Streptococcus c. Group D Streptococcus d. Enterococcus e. Viridans Streptococcus group f. Streptococcus pneumoniae g. Groups C, F, and G Streptococcus</p><p>8. Sketch a flow chart to identify Streptococcus, including specific characteristics and or differential tests to identify each species.</p><p>4/6/2018</p>