<p> Supporting Information</p><p>In vitro biodegradability of bacterial cellulose by cellulase in simulated body fluid and compatibility in vivo </p><p>Baoxiu Wanga,ᴪ，Xiangguo Lvb,ᴪ，Shiyan Chena*，Zhe Lia，Xiaoxiao Suna，Chao Fengb*，Huaping Wanga*, Yuemin Xu a State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Key Laboratory of Textile Science & Technology (Ministry of Education), College of Materials Science and Engineering, Donghua University, Shanghai, 201620, P R China bDepartment of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University , Shanghai, 200233, P R China ᴪCo-first author: These authors contributed equally to the work.</p><p>Email: [email protected], [email protected], [email protected]</p><p>Fig. S1 X-ray diffraction patterns (peak deconvolution method) of (a) BC and cellulase/BC composite for different degradation time: (b) 1 w, (c) 2 w, (d) 4 w (The enzyme concentration and absorption time: 5 mg/mL, 30 min). Fig. S2 FE-SEM images of BC and cellulase/BC composites prepared by freeze-dried method (a) and by absorption method with the absorption time for 30 min (b) at the cellulase concentration of 5 mg/mL. The cellulase content absorbed in BC BCA Protein Assay Kit was used to assess concentration of unbound protein to deduce the cellulase absorbed in BC. In this experiment, the BC pellicles were put into flasks containing cellulase solutions with certain concentration. After certain time, the cellulase/BC composites were rinsed with water to remove cellulases unbounded to BC. The rinsed process was repeated for 5 times. All the washing liquid was collected into the rest of the cellulase solutions which contain endoglucanases, exoglucanases and beta-glucosidases which are some kind of protein. BCA Protein Assay Kit is a simple, highly stable and sensitive method to assess protein concentrations, with detection concentration limit of 25 ug/mL. Under the instruction of Kit, we can obtain the amount of unbound protein. So the concentration of bound protein can be calculated by the total protein minus the unbound protein.</p>