1 SUPPLEMENTARY MATERIAL

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3 Microhabitat segregation and fine ecomorphological dissimilarity between two closely

4 phylogenetically related grazer fishes in an Atlantic Forest stream, Brazil

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6 Environmental Biology of Fishes

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8 Rafael Pereira Leitão, Jorge Ivan Sánchez-Botero, Daniele Kasper, Victor Trivério-

9 Cardoso, Carolina Morais Araújo, Jansen Zuanon & Érica Pellegrini Caramaschi

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11 Corresponding author

12 Rafael P. Leitão

13 Laboratório de Ecologia de Peixes – Universidade Federal do Rio de Janeiro

14 E-mail: [email protected]

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1 24 Online Resource Table 1 Environmental characteristics measured in Forested and

25 Deforested sites of Ouro Stream, Southeastern Brazil

Environmental Forested Deforested variable Mean Range Mean Range Altitude (m) 40 − 25 − Width (m) 6.9 3.0−9.9 7.4 3.5−11.7 Depth (cm) 23.6 2.0−99.0 25.9 2.0−66.0 Current velocity (cm/s) 32.9 0.0−82.1 38.0 0.0−90.2 Canopy cover (%) 75.2 60.2−94.7 0.0 − Water temperature (oC) 22.1 18.3−25.6 22.5 18.1−25.7 Dissolved Oxygen (mg/L) 7.9 7.2−8.4 7.6 7.3−8.1 Conductivity (µS/cm) 40.8 37.1−44.5 45.2 42.9−47.5 pH 7.5 6.9−7.6 7.2 7−7.5 Sand (%) 57.1 − 55.6 − Silt/Clay (%) 7.4 − 24.1 − Boulder/Rubble/Gravel (%) 21.8 − 6.9 − Wood debris/Decaying leaves (%) 13.0 − 2.7 − Grassy vegetation (%) 0.8 − 10.7 − 26

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2 36 Online Resource Table 2 Loadings of Linear Discriminant Analysis on microhabitat

37 variables used by Hisonotus notatus and Parotocinclus maculicauda in Forested and

38 Deforested sites of Ouro Stream, Southeastern Brazil

Microhabitat variable Code Forested Deforested Distance from nearest bank DNB 0.64 0.64 Water depth WTD 0.01 0.45 Focal elevation FEL -0.15 0.81 Average current velocity ACV 0.45 0.44 Focal-point velocity FPV 0.69 0.53 Distance from current shelter DCS 0.39 0.64 Surface-water turbulence SWT 0.63 0.46 Submerged cover SCO -0.02 0.22 Above-water cover ACO 0.02 0.41 Boulder BOU 0.35 − Rubble-gravel RGR 0.14 -0.21 Wood debris WOO -0.17 0.70 Decaying leaves LEA -0.23 -0.13 Grassy vegetation GRA − -0.42 39

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3 48 Online Resource Table 3 Loadings of Principal Component Analysis (PC1 and PC2),

49 mean and range values of ecomorphological traits measured on 30 individuals of

50 Hisonotus notatus and Parotocinclus maculicauda

Ecomorphological H. notatus P. maculicauda Code PC1 PC2 attribute Mean Range Mean Range Body-flatness index BoF 0.83 -0.21 0.75 0.68−0.83 0.62 0.56−0.68 Body depth (mm) BoD 0.70 -0.41 5.64 4.36−7.42 4.72 4.01−5.64 Trunk-shape index TrI 0.49 -0.62 15.51 13.03−18.88 14.37 12.86−16.81 Ventral-flatness index VeF -0.40 -0.27 0.32 0.25−0.41 0.34 0.27−0.39 Peduncle length (mm) PdL -0.19 0.54 10.01 8.29−12.67 9.60 8.39−11.02 Peduncle-flatness index PdF 0.53 -0.37 1.39 1.27−1.56 1.29 1.18−1.50 Oral-disc area (mm2) OdA -0.87 -0.10 16.05 10.63−24.58 21.86 17.44−25.72 Pectoral-fin length (mm) PtL 0.69 -0.37 9.82 7.71−12.42 8.50 7.48−10.08 Pectoral-fin area (mm2) PtA -0.49 -0.44 23.75 12.84−36.56 22.10 15.74−34.64 Pectoral-ray thickness (mm) PtT -0.38 -0.57 0.84 0.62−1.09 0.81 0.66−1.03 Pelvic-fin length (mm) PvL -0.73 -0.32 6.29 4.98−7.70 6.85 6.01−8.11 Pelvic-fin area (mm2) PvA -0.77 -0.39 12.64 6.66−17.64 16.27 10.27−24.18 Pelvic-ray thickness (mm) PvT -0.83 -0.17 0.55 0.41−0.79 0.66 0.57−0.81 Caudal-fork depth (mm) CdF -0.82 -0.03 2.57 1.48−3.41 3.82 2.72−5.45 51

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4 60 Online Resource Fig. 1 Schema of individual microhabitat assessment related to

61 submerged cover (any submerged structure located above focal fish) – SCO; and above-

62 water cover (any structure located up to 50 cm from the water surface above focal fish) –

63 ACO. The cover densities were visually estimated using a square (25 x 25 cm) and

64 categorized as: absent (0%), low (1-25%), medium (26-75%), and high (>75%)

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m c

ACO 0 5

Water surface

SCO

66 25cm

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