<p> 1 SUPPLEMENTARY MATERIAL</p><p>2</p><p>3 Microhabitat segregation and fine ecomorphological dissimilarity between two closely </p><p>4 phylogenetically related grazer fishes in an Atlantic Forest stream, Brazil</p><p>5</p><p>6 Environmental Biology of Fishes</p><p>7</p><p>8 Rafael Pereira Leitão, Jorge Ivan Sánchez-Botero, Daniele Kasper, Victor Trivério-</p><p>9 Cardoso, Carolina Morais Araújo, Jansen Zuanon & Érica Pellegrini Caramaschi</p><p>10</p><p>11 Corresponding author</p><p>12 Rafael P. Leitão</p><p>13 Laboratório de Ecologia de Peixes – Universidade Federal do Rio de Janeiro</p><p>14 E-mail: [email protected]</p><p>15</p><p>16</p><p>17</p><p>18</p><p>19</p><p>20</p><p>21</p><p>22</p><p>23</p><p>1 24 Online Resource Table 1 Environmental characteristics measured in Forested and </p><p>25 Deforested sites of Ouro Stream, Southeastern Brazil</p><p>Environmental Forested Deforested variable Mean Range Mean Range Altitude (m) 40 − 25 − Width (m) 6.9 3.0−9.9 7.4 3.5−11.7 Depth (cm) 23.6 2.0−99.0 25.9 2.0−66.0 Current velocity (cm/s) 32.9 0.0−82.1 38.0 0.0−90.2 Canopy cover (%) 75.2 60.2−94.7 0.0 − Water temperature (oC) 22.1 18.3−25.6 22.5 18.1−25.7 Dissolved Oxygen (mg/L) 7.9 7.2−8.4 7.6 7.3−8.1 Conductivity (µS/cm) 40.8 37.1−44.5 45.2 42.9−47.5 pH 7.5 6.9−7.6 7.2 7−7.5 Sand (%) 57.1 − 55.6 − Silt/Clay (%) 7.4 − 24.1 − Boulder/Rubble/Gravel (%) 21.8 − 6.9 − Wood debris/Decaying leaves (%) 13.0 − 2.7 − Grassy vegetation (%) 0.8 − 10.7 − 26</p><p>27</p><p>28</p><p>29</p><p>30</p><p>31</p><p>32</p><p>33</p><p>34</p><p>35</p><p>2 36 Online Resource Table 2 Loadings of Linear Discriminant Analysis on microhabitat </p><p>37 variables used by Hisonotus notatus and Parotocinclus maculicauda in Forested and </p><p>38 Deforested sites of Ouro Stream, Southeastern Brazil</p><p>Microhabitat variable Code Forested Deforested Distance from nearest bank DNB 0.64 0.64 Water depth WTD 0.01 0.45 Focal elevation FEL -0.15 0.81 Average current velocity ACV 0.45 0.44 Focal-point velocity FPV 0.69 0.53 Distance from current shelter DCS 0.39 0.64 Surface-water turbulence SWT 0.63 0.46 Submerged cover SCO -0.02 0.22 Above-water cover ACO 0.02 0.41 Boulder BOU 0.35 − Rubble-gravel RGR 0.14 -0.21 Wood debris WOO -0.17 0.70 Decaying leaves LEA -0.23 -0.13 Grassy vegetation GRA − -0.42 39</p><p>40</p><p>41</p><p>42</p><p>43</p><p>44</p><p>45</p><p>46</p><p>47</p><p>3 48 Online Resource Table 3 Loadings of Principal Component Analysis (PC1 and PC2), </p><p>49 mean and range values of ecomorphological traits measured on 30 individuals of </p><p>50 Hisonotus notatus and Parotocinclus maculicauda</p><p>Ecomorphological H. notatus P. maculicauda Code PC1 PC2 attribute Mean Range Mean Range Body-flatness index BoF 0.83 -0.21 0.75 0.68−0.83 0.62 0.56−0.68 Body depth (mm) BoD 0.70 -0.41 5.64 4.36−7.42 4.72 4.01−5.64 Trunk-shape index TrI 0.49 -0.62 15.51 13.03−18.88 14.37 12.86−16.81 Ventral-flatness index VeF -0.40 -0.27 0.32 0.25−0.41 0.34 0.27−0.39 Peduncle length (mm) PdL -0.19 0.54 10.01 8.29−12.67 9.60 8.39−11.02 Peduncle-flatness index PdF 0.53 -0.37 1.39 1.27−1.56 1.29 1.18−1.50 Oral-disc area (mm2) OdA -0.87 -0.10 16.05 10.63−24.58 21.86 17.44−25.72 Pectoral-fin length (mm) PtL 0.69 -0.37 9.82 7.71−12.42 8.50 7.48−10.08 Pectoral-fin area (mm2) PtA -0.49 -0.44 23.75 12.84−36.56 22.10 15.74−34.64 Pectoral-ray thickness (mm) PtT -0.38 -0.57 0.84 0.62−1.09 0.81 0.66−1.03 Pelvic-fin length (mm) PvL -0.73 -0.32 6.29 4.98−7.70 6.85 6.01−8.11 Pelvic-fin area (mm2) PvA -0.77 -0.39 12.64 6.66−17.64 16.27 10.27−24.18 Pelvic-ray thickness (mm) PvT -0.83 -0.17 0.55 0.41−0.79 0.66 0.57−0.81 Caudal-fork depth (mm) CdF -0.82 -0.03 2.57 1.48−3.41 3.82 2.72−5.45 51</p><p>52</p><p>53</p><p>54</p><p>55</p><p>56</p><p>57</p><p>58</p><p>59</p><p>4 60 Online Resource Fig. 1 Schema of individual microhabitat assessment related to </p><p>61 submerged cover (any submerged structure located above focal fish) – SCO; and above-</p><p>62 water cover (any structure located up to 50 cm from the water surface above focal fish) – </p><p>63 ACO. The cover densities were visually estimated using a square (25 x 25 cm) and </p><p>64 categorized as: absent (0%), low (1-25%), medium (26-75%), and high (>75%)</p><p>65</p><p> m c</p><p>ACO 0 5</p><p>Water surface </p><p>SCO </p><p>66 25cm </p><p>5</p>