<p>Electronic Supplementary Materials</p><p>Comparison of treatment effects of teriparatide and the bisphosphonate risedronate in an aged, osteopenic, ovariectomized rat model under various clinical conditions</p><p>Ayano Sugie-Oya, Aya Takakura, Ryoko Takao-Kawabata, Hiroko Sano, Yukari Shimazu, Yukihiro</p><p>Isogai, Akira Yamaguchi, Toshinori Ishizuya</p><p>Contents</p><p>Measurement of bone mineral density (BMD) of tibial shaft and femur</p><p>Mechanical properties of femoral shaft and neck</p><p>References</p><p>Table S1. Tibial shaft bone mineral density (BMD) in the preventive and therapeutic studies</p><p>Table S2. Femoral bone mineral density (BMD) in the preventive and therapeutic studies</p><p>Table S3. Femoral bone strength (maximum load) in the preventive and therapeutic studies Measurement of bone mineral density (BMD) of tibial shaft and femur</p><p>The BMD of the tibial shaft and right femur was determined using dual energy X-ray absorptiometry</p><p>(DXA) (DCS-600EX-3R; Aloka, Tokyo, Japan). The BMD of the tibia was measured at the following postoperative times: 0, 1, 2, and 4 months in the preventive study and 0, 12, 13, 14, and 16 months in the therapeutic study. The BMD of the femur was measured at the end of each administration period. The specimens were placed on the scanning table ventral side up and scanned at a pitch of 1 mm and speed of 25 mm/min. The BMD (mg/cm2) was calculated from the bone mineral content (mg) and bone area (cm2). The tibia and femur were divided into three regions for analysis (proximal, shaft, and distal), and the BMD in each region was obtained.</p><p>Mechanical properties of femoral shaft and neck</p><p>The femoral shaft was subjected to a three-point bending test as previously described [1] using the material testing machine at a constant speed of 10 mm/min. The maximum load (N) was recorded. </p><p>After the three-point bending test, mechanical testing of the femoral neck was performed as previously described [2] using the material testing machine. The shaft of the proximal femur was embedded vertically in dental resin. The specimen was placed on a lower platen with the proximal part facing up and compressed with a 4-mm-diameter upper platen at a constant speed of 2 mm/min.</p><p>The maximum load (N) was recorded. </p><p>References</p><p>1. Molster AO (1986) Biomechanical effects of intramedullary reaming and nailing on intact femora in rats. Clin. Orthop. Relat. Res.:278-285 2. Sogaard CH, Wronski TJ, McOsker JE, Mosekilde L (1994) The positive effect of parathyroid hormone on femoral neck bone strength in ovariectomized rats is more pronounced than that of estrogen or bisphosphonates. Endocrinology 134:650-657</p><p>Table S1. Tibial shaft bone mineral density (BMD) in the preventive and therapeutic studies Sham Preventive study BMD (mg/cm2) 0 M 91.4 ± ± 2.4 ** 93.4 1 M 101.2 ± ± 3.2 ** 106.5 2 M 105.1 ± ± 3.5 ** 112.8 4 M 111.2 ± ± 7.4 * 121.8 Change from pretreatment value (Δ%) 1 M 10.8 ± ± 3.0 14.1 2 M 15.1 ± ± 3.9 20.9 4 M 21.7 ± ± 8.2 30.5 Therapeutic study BMD (mg/cm2) 0 M 90.0 ± ± 5.2 90.6 12 M 121.6 ± ± 12.3 119.2 13 M 122.4 ± ± 14.3 121.6 14 M 122.1 ± ± 13.7 124.6 16 M 119.2 ± ± 14.6 126.7 Change from pretreatment value (Δ%) 13 M 0.6 ± ± 3.1 2.0 14 M 0.4 ± ± 4.0 4.6 16 M -2.0 ± ± 4.8 * 6.3 Data are presented as mean ± standard deviation; n = 9–15 *P < 0.05 and **P < 0.01 compared with sham group (t-test) †P < 0.05 and ††P < 0.01 compared with OVX group (Tukey-type test) ‡P < 0.05 and ‡‡P < 0.01 compared with teriparatide group (Tukey-type test) Table S2. Femoral bone mineral density (BMD) in the preventive and therapeutic studies Baseline Sham OVX Preventive study Whole 110.0 ± 4.4 129.5 120 ± 4.8 131.1 Shaft 99.3 ± 3.8 122.4 121.2 ± 5.7 126.0 Proxima 106.2 ± 3.9 126.7 116.9 ± 5.5 127.8 l .6 Baseline-sham Baseline-OVX Sham OVX Therapeutic Whole 137.9 ± 5.6 117.5 ± 6.8 137.1 119 ± 11.7 137.3 study Shaft 139.8 ± 7.3 123.5 ± 7.0 141.0 125.0 ± 12.2 144.6 Proxima 134.5 ± 6.0 111.7 ± 7.3 134.9 115.5 ± 12.5 130.2 l .0 BMD (mg/cm2) data are presented as mean ± standard deviation; n = 9–15 §§P < 0.01 compared with baseline-sham group (t-test) **P < 0.01 compared with sham group (t-test) ††P < 0.01 compared with OVX group (Tukey-type test) ‡P < 0.05 compared with teriparatide group (Tukey-type test)</p><p>Table S3. Femoral bone strength (maximum load) in the preventive and therapeutic studies Baseline Sham OVX Teriparatide Preventive study Shaft 132. ± 6.9 175. ± 13. 178. ± 2 1 Neck 109.6 ± 14.2 128.1 ± 14.6 124.7 ± 2 19 8 8 8 3 3 Baseline-sham Baseline-OVX Sham OVX Teriparatide Therapeutic Shaft 205. ± 29.4 172. ± 27. § 221. ± 29. 171. ± 3 ** 2 study Neck 120.5 ± 22.3 124.6 ± 16.5 132.2 ± 20.5 121.0 ± 2 12 2 7 8 4 6 5 4 Maximum load (N) data are presented as mean ± standard deviation; n = 9–15 §P < 0.05 compared with baseline-sham group (t-test) **P < 0.01 compared with sham group (t-test) †P < 0.05 and ††P < 0.01 compared with OVX group (Tukey-type test)</p>