<p> EXERCISE 26: PROTOZOA/SLIME MOLDS</p><p>What is the Latin meaning of the word Protozoan?</p><p>I. PHYLUM: Rhizopoda (Amoebas) Define: a. pseudopodia- b. phagocytic- c. contractile vacuole-</p><p>PROCEDURE 26.1</p><p>1. From a living culture, prepare a wet mount slide of Amoeba ( or use a prepared slide). 2. Examine under low power ( 4x or 5x). 3. Sketch 2 or 3 Amoeba (about 1” in size) in the space below.</p><p>4. How do Amoeba move?</p><p>5. Can you see any movement? If so describe.</p><p>6. Why is a contractile vacuole a “must have” organelle for aquatic protozoans?</p><p>7. How would you describe the shape of Amoeba?</p><p>II. PHYLUM: Foraminifera (forams) a. Why are forams called shelled Amoebas?</p><p> b. What are foram tests? What are they made of?</p><p>PROCEDURE 26.2</p><p>1. Obtain a prepared slide of some Foraminifers. Look near the edge of coverslip. 2. Sketch 3 different types below (1” size).</p><p>3. Obtain a prepared slide of Radiolaria. 4. Sketch 3 different types below (1” size).</p><p>5. How are the shells of Radiolaria different than Foraminifers? III. PHYLUM: Sarcomastigophora</p><p> a. How do these protozoans move?</p><p> b. What are Trypanasomes?</p><p> c. How do these pathogenic protozoans infect humans?</p><p>PROCEDURE 26.3</p><p>1. Examine a prepared slide of Trypanasoma in a human blood smear. 2. Sketch a Trypanasoma below (1” size).</p><p>3. How does their size compare to a blood cell?</p><p>IV. PHYLUM: Ciliophora (Ciliates) Define: a. cilia-</p><p> b. macronucleus-</p><p> c. micronucleus-</p><p> d. conjugation-</p><p>PROCEDURE: 26.4</p><p>1. Prepare a wet mount slide of Paramecia. 2. How fast do they move compared to Amoeba? </p><p>3. Place a drop of methyl cellulose (Protoslo) on your slide to slow them down for observing their internal structure. Sketch a Paramecium below and label any visible structures.</p><p>4. Examine a prepared slide of conjugating (sexually reproducing) Paramecia. 5. Sketch below.</p><p>6. What are the advantages & disadvantages of conjugation?</p><p>7. Examine a prepared slide of Paramecium during asexual fission. Sketch below.</p><p>8. Examine a prepared slide of the ciliate Vorticella. They attach by a coiled tail to small objects on the bottom of ponds & lakes. Sketch 2 or 3 below.</p><p>9. Of what importance is the protozoan Plasmodium? What is its vector (carrier).</p><p>V. PHYLUM: Myxomycota (slime molds)</p><p> a. Where could you find slime molds growing?</p><p> b. What do they feed on?</p><p>PROCEDURE 26.8</p><p>Follow steps 1-3 page 286 in your lab manual and then sketch a slime mold culture below.</p>
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