<p>Supplementary materials </p><p>Supplementary Table 1.Quality control parameters of exome captured sequence.</p><p>Sample all Covered Exome Re Reads Mapped Mapped Base To Mapped Mapped Average Exome Coverage ad Mapped Reads Genome Base To Base Depth (Base) (%) s To Exome Ratio（% Exome Ratio（%） M ） ap pe d To Ge no m e</p><p>3 25893008 98% 55120236 16874303 30% 4422446913 957079070 21% 36.96 SiBoAoK 5 25807627 98% 49173676 15587895 31% 3962808897 889041348 22% 34.45 e ZTQ 25825834 98% 45233076 15584134 34% 3671687550 881450729 24% 34.13 Supplementary Table 2.Annotation results of the 3 sequenced samples.</p><p>Sample cSNP calls SNP in dbSNP 1000 genome Heterozygous Homozygous</p><p>3 58711 16738 43975 41672 17039 5 54187 16350 42830 38328 15859 ZTQ 52408 15911 41908 37465 14943 Supplementary Table 3.Summary of the types of SNPs from the 3 sequenced samples.</p><p>Sample Non-synonymous synonymou Non-frame Frame Stop Stop s shift shift gain lost insertion insertion</p><p>3 39905 17863 53 159 633 98 5 36654 16660 49 145 596 83 ZTQ 35160 16300 36 131 688 93</p><p>Supplementary Table 4.Independent analysis of Indels from the 3 sequenced samples.</p><p>Sample cINDEL calls Frameshiftingindel Non-frameshiftingindel</p><p>3 264 194 68 5 295 224 71 ZTQ 251 185 66</p><p>Supplement Figure 1. The overlap of SNP from the 3 sequenced samples.</p><p>Supplementary data sheet.The list of 83 candidate mutation positions after rejecting process.</p><p>Chromosome Position Mutation Decription</p><p> chr1 22716505 A zinc finger and BTB domain containing 40 chr1 114325702 C olfactomedin-like 3 chr1 75456773 C solute carrier family 44, member 5 chr1 45859061 C coiled-coil domain containing 17 chr1 18564305 G immunoglobin superfamily, member 21 chr1 232519032 C solute carrier family 35, member F3 chr1 152570575 C ATPase, class I, type 8B, member 2 chr1 163035651 G pre-B-cell leukemia homeobox 1 chr1 9094103 C G protein-coupled receptor 157 mitogen-activated protein kinase-activated chr1 204970018 C protein kinase 2 chr1 39718248 C microtubule-actin crosslinking factor 1 chr1 182181214 G glycosyltransferase 25 domain containing 2 calcium/calmodulin-dependent protein kinase chr10 75282069 C II gamma amyloid beta (A4) precursor protein-binding, chr10 26830006 C family B, member 1 interacting protein chr10 88693223 C multimerin 2 chr10 13892874 A FERM domain containing 4A chr10 106027727 C glutathione S-transferase omega 2 chr10 61708597 C ankyrin 3, node of Ranvier (ankyrin G) chr11 15055464 G calcitonin-related polypeptide beta chr11 57212651 C zinc finger, DHHC-type containing 5 chr11 106315808 C guanylatecyclase 1, soluble, alpha 2 chr11 123014168 C sodium channel, voltage-gated, type III, beta chr12 110587859 G BRCA1 associated protein chr12 48739957 C amiloride-sensitive cation channel 2, neuronal solute carrier family 6 (neurotransmitter chr12 239337 C transporter, GABA), member 13 proteasome (prosome, macropain) 26S chr12 120822117 C subunit, non-ATPase, 9 chr12 92317185 C nudix (nucleoside diphosphate linked moiety X)-type motif 4 leucine-rich repeat containing G protein- chr12 70233200 G coupled receptor 5 chr12 3017446 C TEA domain family member 4 chr12 50664079 T activin A receptor, type IB potassium voltage-gated channel, Shaw- chr12 73730968 G related subfamily, member 2 coagulation factor VII (serum prothrombin chr13 112820776 G conversion accelerator) chr13 112946763 A cullin 4A chr13 19661221 C gap junction protein, beta 2, 26kDa chr13 97450835 T importin 5 chr13 112527821 G ATPase, class VI, type 11A chr16 70453202 G zinc finger protein 821 chr17 9472814 C WD repeat domain 16 chr2 127550751 C bridging integrator 1 chr2 135427616 T cyclin T2 chr2 68898610 C Rho GTPase activating protein 25 carbamoyl-phosphate synthase 1, chr2 211179723 C mitochondrial latent transforming growth factor beta binding chr2 33467850 A protein 1 chr20 4628210 G prion protein chr3 3070492 G contactin 4 sema domain, seven thrombospondin repeats chr3 124117389 C (type 1 and type 1-like) chr3 14730579 C chromosome 3 open reading frame 20 chr3 188927237 G B-cell CLL/lymphoma 6 chr3 52220582 C aminolevulinate, delta-, synthase 1 chr3 13388348 G nucleoporin 210kDa chr3 46282393 G chemokine (C-C motif) receptor 3 chr3 9077036 C SLIT-ROBO Rho GTPase activating protein 3 chr4 94225364 C glutamate receptor, ionotropic, delta 2 chr4 22035006 C G protein-coupled receptor 125 chr4 148683195 C endothelin receptor type A ATPase, aminophospholipid transporter chr4 42272427 G (APLT), class I, type 8A, member 1 chr5 121804244 T synuclein, alpha interacting protein chr5 112467880 C mutated in colorectal cancers chr5 148400432 C SH3 domain and tetratricopeptide repeats 2 chr5 14450227 G triple functional domain (PTPRF interacting) 3-hydroxy-3-methylglutaryl-CoA synthase 1 chr5 43329960 G (soluble) chr6 32024687 C complement factor B chr6 146762462 C glutamate receptor, metabotropic 1 chr6 132312177 G connective tissue growth factor chr6 46955544 G G protein-coupled receptor 116 minichromosome maintenance complex chr7 99534589 G component 7 chr7 120671570 C chromosome 7 open reading frame 58 chr7 148111905 G cullin 1 chr7 103031081 C reelin chr7 16468922 C sclerostin domain containing 1 protein kinase, AMP-activated, gamma 2 non- chr7 150888623 C catalytic subunit chr8 132033417 G adenylatecyclase 8 (brain) chr8 141970011 C PTK2 protein tyrosine kinase 2 chr8 74397787 A retinol dehydrogenase 10 (all-trans) chr8 121336729 G collagen, type XIV, alpha 1 runt-related transcription factor 1; chr8 93073084 G translocated to, 1 (cyclin D-related) chr8 22062205 G leucine-rich repeat LGI family, member 3 chr8 24207621 C ADAM metallopeptidase domain 28 chr9 74007381 C guanine deaminase chr9 130801807 C nucleoporin 188kDa glyoxylatereductase/hydroxypyruvatereductas chr9 37416578 C e chr9 110703565 G inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex- associated protein transient receptor potential cation channel, chr9 72403355 C subfamily M, member 3</p>