<p>Name:______Hour:______</p><p>Enzyme Lab</p><p>Purpose: To determine the job of an enzyme and how enzymes are affected by changes such as temperature and acidity</p><p>Procedure: . Set up the test tubes in a rack as shown in the first column. For example, put peroxide and a chunk of potato in the first test tube. . Liquids should be 1 finger width high. . Record results in second column based on the following scale: - 0 for no bubbles - 3 for fast bubbles - 1 for slow bubbles - 4 for very fast bubbles - 2 for moderate bubbles</p><p>Part I: Different Types of Potato</p><p>1. Peroxide (H2O2) + chunk potato Reaction: 0 1 2 3 4</p><p>2. Peroxide (H2O2) + ground potato Reaction: 0 1 2 3 4</p><p>3. Peroxide (H2O2) + boiled potato Reaction: 0 1 2 3 4</p><p>Part II: Different Types of Liver</p><p>4. Peroxide + chunk liver Reaction: 0 1 2 3 4</p><p>5. Peroxide + ground liver Reaction: 0 1 2 3 4</p><p>6. Peroxide + boiled liver Reaction: 0 1 2 3 4</p><p>Part III: Acid vs. Base</p><p>Allow a chunk of liver to sit in HCl or NaOH for 2-3 minutes before adding peroxide</p><p>7. Peroxide + chunk liver + HCl (acid) Reaction: 0 1 2 3 4</p><p>8. Peroxide + chunk liver + NaOH (base) Reaction: 0 1 2 3 4 Enzyme Lab Analysis</p><p>1. What is an enzyme?</p><p>2. Which substance(s) that we tested today contained enzymes? (Circle) Peroxide Potato Liver</p><p>3. What is a substrate?</p><p>4. What was the substrate that we used in lab today? (Circle) Peroxide Potato Liver</p><p>Part I: Different Types of Potato</p><p>5. Which tube had the greatest reaction? Why?</p><p>6. Why wouldn’t we expect a reaction with the boiled potato?</p><p>Part II: Different Types of Liver</p><p>7. Why would we expect a greater reaction with the ground liver than with the chunk of liver?</p><p>8. Why would we expect a greater reaction with the ground liver than with the boiled liver?</p><p>Part III: Acid vs. Base</p><p>9. What happened to the reaction when acid or base was added to the chunk of liver?</p><p>Summary 10. What does temperature do to enzymes? (Hint: think about the reaction of the boiled potato and boiled liver)</p><p>11. What do acids and bases do to enzymes? (Hint: think about Part III of the lab)</p><p>12. Why might someone with a fever have trouble digesting a large meal?</p>