<p> ANSWERS Process of Science (12.13)</p><p>How Can Gel Electrophoresis Be Used to Analyze DNA?</p><p>Lab Notebook</p><p>Actual Base Pairs (bp) Measured Distance (mm) 23,130 11 9,416 16 6,557 18 4,361 22 2,322 29 2,207 32 564 46</p><p>Distance Migrated (mm) Interpolated Fragment Size (in base pairs) Fragment 1 20 4796 Fragment 2 34 1405 Fragment 3 36 1268</p><p>Distance Migrated (mm) Interpolated Fragment Size (in base pairs) Fragment 1 17 6201 Fragment 2 36 1268</p><p>Questions 1. What is the relationship of migration distance to fragment size?</p><p>Migration distance is inversely proportional to the fragment size.</p><p>2. Approximately how many base pairs are there in the fragment circled in red at left?</p><p>3500</p><p>3. Which lane shows a digest with BamHI only?</p><p>III ______Campbell/Reece/Taylor/Simon: Biology: Concepts & Connections, Sixth Edition, © Pearson Education, Inc., publishing as Benjamin Cummings 4. Which lane shows a digest with EcoRI only?</p><p>II</p><p>5. Which lane shows the fragments produced when the plasmid was incubated with both EcoRI and BamH1?</p><p>IV</p><p>6. A segment of DNA has two restriction sites—I and II. When incubated with restriction enzymes I and II, three fragments will be formed—a, b, and c. Which of the following gels produced by electrophoresis would represent the separation and identity of these fragments?</p><p>B </p><p>______Campbell/Reece/Taylor/Simon: Biology: Concepts & Connections, Sixth Edition, © Pearson Education, Inc., publishing as Benjamin Cummings</p>