<p>SUPPLEMENTAL FIGURES</p><p>Figure S1 – Replication Kinetics of Vif-proficient and Vif-deficient HIV-1IIIB on</p><p>Panels of Human APOBEC3-Expressing SupT11 T Cell Lines </p><p>(A) The replication kinetics of Vif-proficient (gray diamonds) and Vif-deficient</p><p>(black squares) HIV-1IIIB were monitored over three weeks of a spreading infection on panels of SupT11 clones stably expressing each APOBEC3 protein by periodic infection of the reporter line CEM-GFP. The results for four clones from each panel</p><p> are shown here. Vif-proficient HIV-1IIIB replicates with similar kinetics on permissive</p><p>(APOBEC3-low) cell lines, such as CEM-SS and SupT11, and nonpermissive</p><p>(APOBEC3-high) cell lines, such as CEM and H9. Vif-deficient HIV-1IIIB only replicates on permissive cell lines. Stable expression of A3A, A3B, or A3C in the permissive</p><p>SupT11 line results in no inhibition of Vif-deficient HIV-1IIIB replication relative to</p><p>Vif-proficient HIV-1IIIB. On the other hand, Vif-deficient HIV-1IIIB replication is significantly delayed or undetectable on cell lines expressing A3D, A3F, A3G, or A3H.</p><p>In cell lines expressing moderate to high levels of A3H, Vif-proficient HIV-1IIIB</p><p> replication is also undetectable or delayed, reflecting the inability of HIV-1IIIB Vif to completely neutralize C-terminally tagged A3H as previously reported (4, 5) and as in Figure 2C.</p><p>(B) Immunoblots of the SupT11 clones, whose infectivity plots are shown above, demonstrate stable expression of each HA-tagged APOBEC3. The order of the infectivity plots corresponds to the loading order of the samples. Figure S2 – Replication Kinetics of Vif-proficient and Vif-deficient HIV-1IIIB on</p><p>Panels of Rhesus APOBEC3-Expressing SupT11 T Cell Lines</p><p>(A) The replication kinetics of Vif-proficient (gray diamonds) and Vif-deficient</p><p>(black squares) HIV-1IIIB were monitored over three weeks of a spreading infection on panels of SupT11 clones stably expressing each rhesus APOBEC3 protein by periodic infection of the reporter line CEM-GFP. The results for three clones from each panel are shown here. Stable expression of rhesus A3A, A3B, or A3C in the</p><p> permissive SupT11 line results in no inhibition of Vif-deficient HIV-1IIIB replication</p><p> relative to Vif-proficient HIV-1IIIB similar to the vector control lines. On the other</p><p> hand, Vif-deficient HIV-1IIIB replication is undetectable on cell lines expressing</p><p> rhesus A3D, A3F, A3G, or A3H. Vif-proficient HIV-1IIIB replication is similarly suppressed on rhesus A3F and A3H lines and is delayed on rhesus A3G lines,</p><p> reflecting the inability of HIV-1IIIB Vif to completely neutralize these proteins.</p><p>(B) Immunoblots of the SupT11 clones, whose infectivity plots are shown above, demonstrate stable expression of each HA-tagged rhesus APOBEC3. The order of the infectivity plots corresponds to the loading order of the samples.</p>