<p>Table S5. Strains Plasmid Description Reference E. coli BL21(DE3) pVL847 RocR Purification of His-MBP-tagged PA14 RocR This study E. coli T7Iq pVL847 Gn GW VC0341 Purification of His-MBP-tagged V. cholerae El Tor N16961 Orn This study E. coli BL21(DE3) pVL847 PA14 orn Purification of His-MBP-tagged PA14 Orn This study E. coli BL21(DE3) pVL847 D11A Purification of His-MBP-tagged PA14 Orn D11A This study E. coli BL21(DE3) pVL847 E13A Purification of His-MBP-tagged PA14 Orn E13A This study E. coli BL21(DE3) pVL847 D111A Purification of His-MBP-tagged PA14 Orn D111A This study E. coli BL21(DE3) pVL847 H157A Purification of His-MBP-tagged PA14 Orn H157A This study E. coli BL21(DE3) pVL847 D162A Purification of His-MBP-tagged PA14 Orn D162A This study PA14 Wild type (1) PA14 ∆orn In-frame deletion of orn, oligoribonuclease This study PA14 ∆pel In-frame deletion of the pel operon (pelA-G) (2) PA14 ∆orn∆pel In-frame deletion of orn and the pel operon This study PA14 cat::tn MrT7 transpson insertion in cat, chloramphenicol acetyltransferase (3) PA14 orn::tn MrT7 transposon insertion in orn, oligoribonuclease (3) PA14 PA4108::tn MrT7 transposion insertion in PA4108, HD-GYP domain (3) PA14 PA4781::tn MrT7 transposion insertion in PA4781, HD-GYP domain (3) PA14 pMMB Wild type with empty vector This study PA14 ∆orn pMMB In-frame deletion of orn with empty vector This study PA14 ∆orn pMMB orn Complementation with wild type orn This study PA14 ∆orn pMMB D11A Complementation with D11A allele orn This study PA14 ∆orn pMMB E13A Complementation with E13A allele orn This study PA14 ∆orn pMMB D111A Complementation with D111A allele orn This study PA14 ∆orn pMMB H157A Complementation with H157A allele orn This study PA14 ∆orn pMMB D162A Complementation with D162A allele orn This study</p><p>References 1. Rahme LG, et al. (1995) Common virulence factors for bacterial pathogenicity in plants and animals. Science 268(5219):1899-1902. 2. Cole SJ, Records AR, Orr MW, Linden SB, & Lee VT (2014) Catheter-associated urinary tract infection by Pseudomonas aeruginosa is mediated by exopolysaccharide-independent biofilms. Infect Immun 82(5):2048-2058. 3. Liberati NT, et al. (2006) An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A 103(8):2833-2838.</p><p>2</p>