<p>Online Resource 1 GenBank accession numbers for each gene/region by organelle</p><p>Article title: Biodiversity assessment using next-generation sequencing: comparison of phylogenetic and functional diversity between Nebraska grasslands Journal name: Plant Systematics and Evolution Authors: Dakota L. Ahrendsen, Shelly K. Aust, and P. Roxanne Kellar Affiliation: Department of Biology, University of Nebraska at Omaha, 6001 Dodge Street, Omaha, NE 68182 USA Author for correspondence: [email protected] Plastid (cpDNA): page 1 of 5 Species atpA atpB atpE atpF atpH atpI ccsA cemA matK ndhA ndhB ndhC ndhD ndhE Amorpha canescens a a a a a a a a a a a a a a Amorpha fruticosa a a a a a a a a a a a a a a Astragalus canadensis a a a a a a a a a a a a a a Astragalus crassicarpus a a a a a a a a a a a a a a KT45662 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT456898 KT456928 KT456957 KT456987 KT457017 KT457046 Callirhoe involucrata 9 9 9 9 9 9 9 9 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456899 KT456929 KT456958 KT456988 KT457018 KT457047 Cannabis sativa 0 0 0 0 0 0 0 0 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456900 KT456930 KT456959 KT456989 KT457019 KT457048 Capsella bursa-pastoris 1 1 1 1 1 1 1 1 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456901 KT456931 KT456960 KT456990 KT457020 KT457049 Ceanothus herbaceous 2 2 2 2 2 2 2 2 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456902 KT456932 KT456961 KT456991 KT457021 KT457050 Chamaecrista fasciculata 3 3 3 3 3 3 3 3 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456903 KT456933 KT456962 KT456992 KT457022 KT457051 Chorispora tenella 4 4 4 4 4 4 4 4 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456904 KT456934 KT456963 KT456993 KT457023 KT457052 Cleome serrulata 5 5 5 5 5 5 5 5 Comandra umbellata subsp. umbellata b b b b b b b b b b b b b b KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456905 KT456935 KT456964 KT456994 KT457024 KT457053 Croton texensis 6 6 6 6 6 6 6 6 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456906 KT456936 KT456965 KT456995 KT457025 KT457054 Desmodium illinoense 7 7 7 7 7 7 7 7 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456907 KT456937 KT456966 KT456996 KT457026 KT457055 Euphorbia esula 8 8 8 8 8 8 8 8 KT45663 KT45666 KT45669 KT45672 KT45675 KT45678 KT45681 KT45684 KT456908 KT456938 KT456967 KT456997 KT457027 KT457056 Euphorbia marginata 9 9 9 9 9 9 9 9 KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456909 KT456939 KT456968 KT456998 KT457028 KT457057 Gaura coccinea 0 0 0 0 0 0 0 0 KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456910 KT456940 KT456969 KT456999 KT457029 KT457058 Glycyrrhiza lepidota 1 1 1 1 1 1 1 1 Lathyrus decaphyllus a a a a a a a a a a a a a a KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456911 KT456941 KT456970 KT457000 KT457030 KT457059 Lepidium densiflorum 2 2 2 2 2 2 2 2 KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456912 KT456942 KT456971 KT457001 KT457031 KT457060 Lespedeza capitata 3 3 3 3 3 3 3 3 Medicago lupulina a a a a a a a a a a a a a a KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456913 KT456943 KT456972 KT457002 KT457032 KT457061 Medicago sativa 4 4 4 4 4 4 4 4 Melilotus albus a a a a a a a a a a a a a a Melilotus officinalis a a a a a a a a a a a a a a KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456914 KT456944 KT456973 KT457003 KT457033 KT457062 Oenothera nuttallii 5 5 5 5 5 5 5 5 KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456915 KT456945 KT456974 KT457004 KT457034 KT457063 Oxalis dillenii 6 6 6 6 6 6 6 6 Oxytropis lambertii a a a a a a a a a a a a a a Pediomelum argophyllum a a a a a a a a a a a a a a Pediomelum digitatum a a a a a a a a a a a a a a KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456916 KT456946 KT456975 KT457005 KT457035 KT457064 Pediomelum esculentum 7 7 7 7 7 7 7 7 KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456917 KT456947 KT456976 KT457006 KT457036 KT457065 Physaria ludoviciana 8 8 8 8 8 8 8 8 KT45664 KT45667 KT45670 KT45673 KT45676 KT45679 KT45682 KT45685 KT456918 c KT456977 KT457007 c KT457066 Polygala alba 9 9 9 9 9 9 9 9 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456919 KT456948 KT456978 KT457008 KT457037 KT457067 Prunus virginiana 0 0 0 0 0 0 0 0 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456920 KT456949 KT456979 KT457009 KT457038 KT457068 Psoralidium lanceolatum 1 1 1 1 1 1 1 1 Psoralidium tenuiflorum a a a a a a a a a a a a a a KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456921 KT456950 KT456980 KT457010 KT457039 KT457069 Salix exigua subsp. interior 2 2 2 2 2 2 2 2 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456922 KT456951 KT456981 KT457011 KT457040 KT457070 Senna marilandica 3 3 3 3 3 3 3 3 Silene antirrhina b b b b b b b b b b b b b b Silene vulgaris b b b b b b b b b b b b b b KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456923 KT456952 KT456982 KT457012 KT457041 KT457071 Sphaeralcea coccinea 4 4 4 4 4 4 4 4 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456924 KT456953 KT456983 KT457013 KT457042 KT457072 Vicia americana var. minor 5 5 5 5 5 5 5 5 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456925 KT456954 KT456984 KT457014 KT457043 KT457073 Vicia villosa 6 6 6 6 6 6 6 6 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456926 KT456955 KT456985 KT457015 KT457044 KT457074 Viola bicolor 7 7 7 7 7 7 7 7 KT45665 KT45668 KT45671 KT45674 KT45677 KT45680 KT45683 KT45686 KT456927 KT456956 KT456986 KT457016 KT457045 KT457075 Viola pedatifida 8 8 8 8 8 8 8 8 a See Kellar et al. 2015 for GenBank accession number b See Aust et al. 2015 for GenBank accession number c DNA region not recovered from Illumina sequencing reads; therefore, coded as missing data in phylogenetic analyses Plastid (cpDNA): page 2 of 5 Species ndhF ndhG ndhH ndhI ndhJ ndhK petA petB petG petL petN psaA psaB psaC Amorpha canescens a a a a a a a a a a a a a a Amorpha fruticosa a a a a a a a a a a a a a a Astragalus canadensis a a a a a a a a a a a a a a Astragalus crassicarpus a a a a a a a a a a a a a a KT45707 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT457344 KT457374 KT457404 KT457434 KT457464 KT457494 Callirhoe involucrata 6 5 4 4 4 4 4 4 KT45707 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT457345 KT457375 KT457405 KT457435 KT457465 KT457495 Cannabis sativa 7 6 5 5 5 5 5 5 KT45707 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT457346 KT457376 KT457406 KT457436 KT457466 KT457496 Capsella bursa-pastoris 8 7 6 6 6 6 6 6 KT45707 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT457347 KT457377 KT457407 KT457437 KT457467 KT457497 Ceanothus herbaceous 9 8 7 7 7 7 7 7 KT45708 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT457348 KT457378 KT457408 KT457438 KT457468 KT457498 Chamaecrista fasciculata 0 9 8 8 8 8 8 8 KT45708 KT45711 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT457349 KT457379 KT457409 KT457439 KT457469 KT457499 Chorispora tenella 1 0 9 9 9 9 9 9 KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457350 KT457380 KT457410 KT457440 KT457470 KT457500 Cleome serrulata 2 1 0 0 0 0 0 0 Comandra umbellata subsp. umbellata b b b b b b b b b b b b b b KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457351 KT457381 KT457411 KT457441 KT457471 KT457501 Croton texensis 3 2 1 1 1 1 1 1 KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457352 KT457382 KT457412 KT457442 KT457472 KT457502 Desmodium illinoense 4 3 2 2 2 2 2 2 KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457353 KT457383 KT457413 KT457443 KT457473 KT457503 Euphorbia esula 5 4 3 3 3 3 3 3 KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457354 KT457384 KT457414 KT457444 KT457474 KT457504 Euphorbia marginata 6 5 4 4 4 4 4 4 KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457355 KT457385 KT457415 KT457445 KT457475 KT457505 Gaura coccinea 7 6 5 5 5 5 5 5 KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457356 KT457386 KT457416 KT457446 KT457476 KT457506 Glycyrrhiza lepidota 8 7 6 6 6 6 6 6 Lathyrus decaphyllus a a a a a a a a a a a a a a KT45708 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457357 KT457387 KT457417 KT457447 KT457477 KT457507 Lepidium densiflorum 9 8 7 7 7 7 7 7 KT45709 KT45711 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457358 KT457388 KT457418 KT457448 KT457478 KT457508 Lespedeza capitata 0 9 8 8 8 8 8 8 Medicago lupulina a a a a a a a a a a a a a a KT45709 KT45712 KT45714 KT45717 KT45720 KT45723 KT45726 KT45729 KT457359 KT457389 KT457419 KT457449 KT457479 KT457509 Medicago sativa 1 0 9 9 9 9 9 9 Melilotus albus a a a a a a a a a a a a a a Melilotus officinalis a a a a a a a a a a a a a a KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457360 KT457390 KT457420 KT457450 KT457480 KT457510 Oenothera nuttallii 2 1 0 0 0 0 0 0 KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457361 KT457391 KT457421 KT457451 KT457481 KT457511 Oxalis dillenii 3 2 1 1 1 1 1 1 Oxytropis lambertii a a a a a a a a a a a a a a Pediomelum argophyllum a a a a a a a a a a a a a a Pediomelum digitatum a a a a a a a a a a a a a a KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457362 KT457392 KT457422 KT457452 KT457482 KT457512 Pediomelum esculentum 4 3 2 2 2 2 2 2 KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457363 KT457393 KT457423 KT457453 KT457483 KT457513 Physaria ludoviciana 5 4 3 3 3 3 3 3 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 c c KT457364 KT457394 KT457424 KT457454 KT457484 KT457514 Polygala alba 4 4 4 4 4 4 KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457365 KT457395 KT457425 KT457455 KT457485 KT457515 Prunus virginiana 6 5 5 5 5 5 5 5 KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457366 KT457396 KT457426 KT457456 KT457486 KT457516 Psoralidium lanceolatum 7 6 6 6 6 6 6 6 Psoralidium tenuiflorum a a a a a a a a a a a a a a KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457367 KT457397 KT457427 KT457457 KT457487 KT457517 Salix exigua subsp. interior 8 7 7 7 7 7 7 7 KT45709 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457368 KT457398 KT457428 KT457458 KT457488 KT457518 Senna marilandica 9 8 8 8 8 8 8 8 Silene antirrhina b b b b b b b b b b b b b b Silene vulgaris b b b b b b b b b b b b b b KT45710 KT45712 KT45715 KT45718 KT45721 KT45724 KT45727 KT45730 KT457369 KT457399 KT457429 KT457459 KT457489 KT457519 Sphaeralcea coccinea 0 9 9 9 9 9 9 9 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT45731 KT457370 KT457400 KT457430 KT457460 KT457490 KT457520 Vicia americana var. minor 1 0 0 0 0 0 0 0 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT45731 KT457371 KT457401 KT457431 KT457461 KT457491 KT457521 Vicia villosa 2 1 1 1 1 1 1 1 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT45731 KT457372 KT457402 KT457432 KT457462 KT457492 KT457522 Viola bicolor 3 2 2 2 2 2 2 2 KT45710 KT45713 KT45716 KT45719 KT45722 KT45725 KT45728 KT45731 KT457373 KT457403 KT457433 KT457463 KT457493 KT457523 Viola pedatifida 4 3 3 3 3 3 3 3 a See Kellar et al. 2015 for GenBank accession number b See Aust et al. 2015 for GenBank accession number c DNA region not recovered from Illumina sequencing reads; therefore, coded as missing data in phylogenetic analyses Plastid (cpDNA): page 3 of 5 Species psaI psaJ psbA psbB psbC psbD psbE psbF psbI psbJ psbK psbL psbM psbN Amorpha canescens a a a a a a a a a a a a a a Amorpha fruticosa a a a a a a a a a a a a a a Astragalus canadensis a a a a a a a a a a a a a a Astragalus crassicarpus a a a a a a a a a a a a a a KT45752 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT457794 KT457824 KT457854 KT457884 KT457914 KT457944 Callirhoe involucrata 4 4 4 4 4 4 4 4 KT45752 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT457795 KT457825 KT457855 KT457885 KT457915 KT457945 Cannabis sativa 5 5 5 5 5 5 5 5 KT45752 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT457796 KT457826 KT457856 KT457886 KT457916 KT457946 Capsella bursa-pastoris 6 6 6 6 6 6 6 6 KT45752 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT457797 KT457827 KT457857 KT457887 KT457917 KT457947 Ceanothus herbaceous 7 7 7 7 7 7 7 7 KT45752 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT457798 KT457828 KT457858 KT457888 KT457918 KT457948 Chamaecrista fasciculata 8 8 8 8 8 8 8 8 KT45752 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT457799 KT457829 KT457859 KT457889 KT457919 KT457949 Chorispora tenella 9 9 9 9 9 9 9 9 KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457800 KT457830 KT457860 KT457890 KT457920 KT457950 Cleome serrulata 0 0 0 0 0 0 0 0 Comandra umbellata subsp. umbellata b b b b b b b b b b b b b b KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457801 KT457831 KT457861 KT457891 KT457921 KT457951 Croton texensis 1 1 1 1 1 1 1 1 KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457802 KT457832 KT457862 KT457892 KT457922 KT457952 Desmodium illinoense 2 2 2 2 2 2 2 2 KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457803 KT457833 KT457863 KT457893 KT457923 KT457953 Euphorbia esula 3 3 3 3 3 3 3 3 KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457804 KT457834 KT457864 KT457894 KT457924 KT457954 Euphorbia marginata 4 4 4 4 4 4 4 4 KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457805 KT457835 KT457865 KT457895 KT457925 KT457955 Gaura coccinea 5 5 5 5 5 5 5 5 KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457806 KT457836 KT457866 KT457896 KT457926 KT457956 Glycyrrhiza lepidota 6 6 6 6 6 6 6 6 Lathyrus decaphyllus a a a a a a a a a a a a a a KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457807 KT457837 KT457867 KT457897 KT457927 KT457957 Lepidium densiflorum 7 7 7 7 7 7 7 7 KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457808 KT457838 KT457868 KT457898 KT457928 KT457958 Lespedeza capitata 8 8 8 8 8 8 8 8 Medicago lupulina a a a a a a a a a a a a a a KT45753 KT45756 KT45759 KT45762 KT45765 KT45768 KT45771 KT45774 KT457809 KT457839 KT457869 KT457899 KT457929 KT457959 Medicago sativa 9 9 9 9 9 9 9 9 Melilotus albus a a a a a a a a a a a a a a Melilotus officinalis a a a a a a a a a a a a a a KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457810 KT457840 KT457870 KT457900 KT457930 KT457960 Oenothera nuttallii 0 0 0 0 0 0 0 0 KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457811 KT457841 KT457871 KT457901 KT457931 KT457961 Oxalis dillenii 1 1 1 1 1 1 1 1 Oxytropis lambertii a a a a a a a a a a a a a a Pediomelum argophyllum a a a a a a a a a a a a a a Pediomelum digitatum a a a a a a a a a a a a a a KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457812 KT457842 KT457872 KT457902 KT457932 KT457962 Pediomelum esculentum 2 2 2 2 2 2 2 2 KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457813 KT457843 KT457873 KT457903 KT457933 KT457963 Physaria ludoviciana 3 3 3 3 3 3 3 3 KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457814 KT457844 KT457874 KT457904 KT457934 KT457964 Polygala alba 4 4 4 4 4 4 4 4 KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457815 KT457845 KT457875 KT457905 KT457935 KT457965 Prunus virginiana 5 5 5 5 5 5 5 5 KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457816 KT457846 KT457876 KT457906 KT457936 KT457966 Psoralidium lanceolatum 6 6 6 6 6 6 6 6 Psoralidium tenuiflorum a a a a a a a a a a a a a a KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457817 KT457847 KT457877 KT457907 KT457937 KT457967 Salix exigua subsp. interior 7 7 7 7 7 7 7 7 KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457818 KT457848 KT457878 KT457908 KT457938 KT457968 Senna marilandica 8 8 8 8 8 8 8 8 Silene antirrhina b b b b b b b b b b b b b b Silene vulgaris b b b b b b b b b b b b b b KT45754 KT45757 KT45760 KT45763 KT45766 KT45769 KT45772 KT45775 KT457819 KT457849 KT457879 KT457909 KT457939 KT457969 Sphaeralcea coccinea 9 9 9 9 9 9 9 9 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT45776 KT457820 KT457850 KT457880 KT457910 KT457940 KT457970 Vicia americana var. minor 0 0 0 0 0 0 0 0 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT45776 KT457821 KT457851 KT457881 KT457911 KT457941 KT457971 Vicia villosa 1 1 1 1 1 1 1 1 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT45776 KT457822 KT457852 KT457882 KT457912 KT457942 KT457972 Viola bicolor 2 2 2 2 2 2 2 2 KT45755 KT45758 KT45761 KT45764 KT45767 KT45770 KT45773 KT45776 KT457823 KT457853 KT457883 KT457913 KT457943 KT457973 Viola pedatifida 3 3 3 3 3 3 3 3 a See Kellar et al. 2015 for GenBank accession number b See Aust et al. 2015 for GenBank accession number c DNA region not recovered from Illumina sequencing reads; therefore, coded as missing data in phylogenetic analyses Plastid (cpDNA): page 4 of 5 Species psbT psbZ rbcL rpl14 rpl16 rpl2 rpl20 rpl23 rpl33 rpl36 rpoA rpoB rpoC1 rpoC2 Amorpha canescens a a a a a a a a a a a a a a Amorpha fruticosa a a a a a a a a a a a a a a Astragalus canadensis a a a a a a a a a a a a a a Astragalus crassicarpus a a a a a a a a a a a a a a KT45797 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT458243 KT458273 KT458303 KT458333 KT458363 KT458393 Callirhoe involucrata 4 4 4 4 4 4 4 3 KT45797 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT458244 KT458274 KT458304 KT458334 KT458364 KT458394 Cannabis sativa 5 5 5 5 5 5 5 4 KT45797 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT458245 KT458275 KT458305 KT458335 KT458365 KT458395 Capsella bursa-pastoris 6 6 6 6 6 6 6 5 KT45797 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT458246 KT458276 KT458306 KT458336 KT458366 KT458396 Ceanothus herbaceous 7 7 7 7 7 7 7 6 KT45797 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT458247 KT458277 KT458307 KT458337 KT458367 KT458397 Chamaecrista fasciculata 8 8 8 8 8 8 8 7 KT45797 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT458248 KT458278 KT458308 KT458338 KT458368 KT458398 Chorispora tenella 9 9 9 9 9 9 9 8 KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45818 KT458249 KT458279 KT458309 KT458339 KT458369 KT458399 Cleome serrulata 0 0 0 0 0 0 0 9 Comandra umbellata subsp. umbellata b b b b b b b b b b b b b b KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458250 KT458280 KT458310 KT458340 KT458370 KT458400 Croton texensis 1 1 1 1 1 1 1 0 KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458251 KT458281 KT458311 KT458341 KT458371 KT458401 Desmodium illinoense 2 2 2 2 2 2 2 1 KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458252 KT458282 KT458312 KT458342 KT458372 KT458402 Euphorbia esula 3 3 3 3 3 3 3 2 KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458253 KT458283 KT458313 KT458343 KT458373 KT458403 Euphorbia marginata 4 4 4 4 4 4 4 3 KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458254 KT458284 KT458314 KT458344 KT458374 KT458404 Gaura coccinea 5 5 5 5 5 5 5 4 KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458255 KT458285 KT458315 KT458345 KT458375 KT458405 Glycyrrhiza lepidota 6 6 6 6 6 6 6 5 Lathyrus decaphyllus a a a a a a a a a a a a a a KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458256 KT458286 KT458316 KT458346 KT458376 KT458406 Lepidium densiflorum 7 7 7 7 7 7 7 6 KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458257 KT458287 KT458317 KT458347 KT458377 KT458407 Lespedeza capitata 8 8 8 8 8 8 8 7 Medicago lupulina a a a a a a a a a a a a a a KT45798 KT45801 KT45804 KT45807 KT45810 KT45813 KT45816 KT45819 KT458258 KT458288 KT458318 KT458348 KT458378 KT458408 Medicago sativa 9 9 9 9 9 9 9 8 Melilotus albus a a a a a a a a a a a a a a Melilotus officinalis a a a a a a a a a a a a a a KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45819 KT458259 KT458289 KT458319 KT458349 KT458379 KT458409 Oenothera nuttallii 0 0 0 0 0 0 0 9 KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458260 KT458290 KT458320 KT458350 KT458380 KT458410 Oxalis dillenii 1 1 1 1 1 1 1 0 Oxytropis lambertii a a a a a a a a a a a a a a Pediomelum argophyllum a a a a a a a a a a a a a a Pediomelum digitatum a a a a a a a a a a a a a a KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458261 KT458291 KT458321 KT458351 KT458381 KT458411 Pediomelum esculentum 2 2 2 2 2 2 2 1 KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458262 KT458292 KT458322 KT458352 KT458382 KT458412 Physaria ludoviciana 3 3 3 3 3 3 3 2 KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45820 c KT458263 KT458293 KT458323 KT458353 KT458383 KT458413 Polygala alba 4 4 4 4 4 4 3 KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458264 KT458294 KT458324 KT458354 KT458384 KT458414 Prunus virginiana 5 5 5 5 5 5 4 4 KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458265 KT458295 KT458325 KT458355 KT458385 KT458415 Psoralidium lanceolatum 6 6 6 6 6 6 5 5 Psoralidium tenuiflorum a a a a a a a a a a a a a a KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458266 KT458296 KT458326 KT458356 KT458386 KT458416 Salix exigua subsp. interior 7 7 7 7 7 7 6 6 KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458267 KT458297 KT458327 KT458357 KT458387 KT458417 Senna marilandica 8 8 8 8 8 8 7 7 Silene antirrhina b b b b b b b b b b b b b b Silene vulgaris b b b b b b b b b b b b b b KT45799 KT45802 KT45805 KT45808 KT45811 KT45814 KT45817 KT45820 KT458268 KT458298 KT458328 KT458358 KT458388 KT458418 Sphaeralcea coccinea 9 9 9 9 9 9 8 8 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45817 KT45820 KT458269 KT458299 KT458329 KT458359 KT458389 KT458419 Vicia americana var. minor 0 0 0 0 0 0 9 9 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT45821 KT458270 KT458300 KT458330 KT458360 KT458390 KT458420 Vicia villosa 1 1 1 1 1 1 0 0 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT45821 KT458271 KT458301 KT458331 KT458361 KT458391 KT458421 Viola bicolor 2 2 2 2 2 2 1 1 KT45800 KT45803 KT45806 KT45809 KT45812 KT45815 KT45818 KT45821 KT458272 KT458302 KT458332 KT458362 KT458392 KT458422 Viola pedatifida 3 3 3 3 3 3 2 2 a See Kellar et al. 2015 for GenBank accession number b See Aust et al. 2015 for GenBank accession number c DNA region not recovered from Illumina sequencing reads; therefore, coded as missing data in phylogenetic analyses Plastid (cpDNA): page 5 of 5 r p rps11 rps12 rps15 rps16 rps18 rps19 rps2 rps3 rps7 rps8 ycf3 Species s 4</p><p>Amorpha canescens a a a a a a a a a a a a </p><p>Amorpha fruticosa a a a a a a a a a a a a </p><p>Astragalus canadensis a a a a a a a a a a a a </p><p>Astragalus crassicarpus a a a a a a a a a a a a </p><p>K T 4 KT45842 KT45845 KT45855 KT45858 KT45864 5 KT458 KT458513 c KT458611 KT458701 KT458761 Callirhoe involucrata 3 3 1 1 1 8 731 6 7 1 K T 4 KT45842 KT45845 KT45855 KT45858 KT45864 5 KT458 KT458514 c KT458612 KT458702 KT458762 Cannabis sativa 4 4 2 2 2 8 732 6 7 2 K T 4 KT45842 KT45845 KT45855 KT45858 KT45864 5 KT458 KT458515 KT458543 KT458613 KT458703 KT458763 Capsella bursa-pastoris 5 5 3 3 3 8 733 6 7 3 K T 4 KT45842 KT45845 KT45855 KT45858 KT45864 5 KT458 KT458516 c KT458614 KT458704 KT458764 Ceanothus herbaceous 6 6 4 4 4 8 734 6 7 4 K T 4 KT45842 KT45845 KT45855 KT45858 KT45864 5 KT458 KT458517 c KT458615 KT458705 KT458765 Chamaecrista fasciculata 7 7 5 5 5 8 735 6 7 5 K T 4 KT45842 KT45845 KT45855 KT45858 KT45864 5 KT458 KT458518 KT458544 KT458616 KT458706 KT458766 Chorispora tenella 8 8 6 6 6 8 736 6 7 6 K T 4 KT45842 KT45845 KT45855 KT45858 KT45864 5 KT458 KT458519 KT458545 KT458617 KT458707 KT458767 Cleome serrulata 9 9 7 7 7 8 737 6 7 7</p><p>Comandra umbellata subsp. umbellata b b b b b b b b b b b b </p><p>K T 4 KT45843 KT45846 KT45855 KT45858 KT45864 5 KT458 KT458520 c KT458618 KT458708 KT458768 Croton texensis 0 0 8 8 8 8 738 6 7 8 K T 4 KT45843 KT45846 KT45855 KT45858 KT45864 5 KT458 KT458521 c KT458619 KT458709 KT458769 Desmodium illinoense 1 1 9 9 9 8 739 6 7 9 K T 4 KT45843 KT45846 KT45856 KT45859 KT45865 5 KT458 KT458522 c KT458620 KT458710 KT458770 Euphorbia esula 2 2 0 0 0 8 740 6 8 0 K T 4 KT45843 KT45846 KT45856 KT45859 KT45865 5 KT458 KT458523 c KT458621 KT458711 KT458771 Euphorbia marginata 3 3 1 1 1 8 741 6 8 1 K T 4 KT45843 KT45846 KT45856 KT45859 KT45865 5 KT458 KT458524 KT458546 KT458622 KT458712 KT458772 Gaura coccinea 4 4 2 2 2 8 742 6 8 2 K T 4 KT45843 KT45846 KT45856 KT45859 KT45865 5 KT458 KT458525 c KT458623 KT458713 KT458773 Glycyrrhiza lepidota 5 5 3 3 3 8 743 6 8 3</p><p>Lathyrus decaphyllus a a a a a a a a a a a a </p><p>K T 4 KT45843 KT45846 KT45856 KT45859 KT45865 5 KT458 KT458526 KT458547 KT458624 KT458714 KT458774 Lepidium densiflorum 6 6 4 4 4 8 744 6 8 4 K T 4 KT45843 KT45846 KT45856 KT45859 KT45865 5 KT458 KT458527 c KT458625 KT458715 KT458775 Lespedeza capitata 7 7 5 5 5 8 745 6 8 5</p><p>Medicago lupulina a a a a a a a a a a a a </p><p>Medicago sativa KT45843 KT45846 KT458528 c KT45856 KT45859 KT458626 KT45865 K KT458716 KT458 KT458776 8 8 6 6 6 T 746 4 5 8 6 8 6</p><p>Melilotus albus a a a a a a a a a a a a </p><p>Melilotus officinalis a a a a a a a a a a a a </p><p>K T 4 KT45843 KT45846 KT45856 KT45859 KT45865 5 KT458 KT458529 KT458548 KT458627 KT458717 KT458777 Oenothera nuttallii 9 9 7 7 7 8 747 6 8 7 K T 4 KT45844 KT45847 KT45856 KT45859 KT45865 5 KT458 KT458530 c KT458628 KT458718 KT458778 Oxalis dillenii 0 0 8 8 8 8 748 6 8 8</p><p>Oxytropis lambertii a a a a a a a a a a a a </p><p>Pediomelum argophyllum a a a a a a a a a a a a </p><p>Pediomelum digitatum a a a a a a a a a a a a </p><p>K T 4 KT45844 KT45847 KT45856 KT45859 KT45865 5 KT458 KT458531 c KT458629 KT458719 KT458779 Pediomelum esculentum 1 1 9 9 9 8 749 6 8 9 K T 4 KT45844 KT45847 KT45857 KT45860 KT45866 5 KT458 KT458532 KT458549 KT458630 KT458720 KT458780 Physaria ludoviciana 2 2 0 0 0 8 750 6 9 0 K T 4 KT45844 KT45847 KT45857 KT45860 KT45866 5 KT458 KT458533 c KT458631 KT458721 KT458781 Polygala alba 3 3 1 1 1 8 751 6 9 1 K T 4 KT45844 KT45847 KT45857 KT45860 KT45866 5 KT458 KT458534 KT458550 KT458632 KT458722 KT458782 Prunus virginiana 4 4 2 2 2 8 752 6 9 2 K T 4 KT45844 KT45847 KT45857 KT45860 KT45866 5 KT458 KT458535 c KT458633 KT458723 KT458783 Psoralidium lanceolatum 5 5 3 3 3 8 753 6 9 3</p><p>Psoralidium tenuiflorum a a a a a a a a a a a a </p><p>Salix exigua subsp. interior KT45844 KT45847 KT458536 c KT45857 KT45860 KT458634 KT45866 K KT458724 KT458 KT458784 T 4 5 6 6 4 4 4 8 754 6 9 4 K T 4 KT45844 KT45847 KT45857 KT45860 KT45866 5 KT458 KT458537 c KT458635 KT458725 KT458785 Senna marilandica 7 7 5 5 5 8 755 6 9 5</p><p>Silene antirrhina b b b b b b b b b b b b </p><p>Silene vulgaris b b b b b b b b b b b b </p><p>K T 4 KT45844 KT45847 KT45857 KT45860 KT45866 5 KT458 KT458538 c KT458636 KT458726 KT458786 Sphaeralcea coccinea 8 8 6 6 6 8 756 6 9 6 K T 4 KT45844 KT45847 KT45857 KT45860 KT45866 5 KT458 KT458539 c KT458637 KT458727 KT458787 Vicia americana var. minor 9 9 7 7 7 8 757 6 9 7 K T 4 KT45845 KT45848 KT45857 KT45860 KT45866 5 KT458 KT458540 c KT458638 KT458728 KT458788 Vicia villosa 0 0 8 8 8 8 758 6 9 8 K T 4 KT45845 KT45848 KT45857 KT45860 KT45866 5 KT458 KT458541 c KT458639 KT458729 KT458789 Viola bicolor 1 1 9 9 9 8 759 6 9 9 K T 4 KT45845 KT45848 KT45858 KT45861 KT45867 5 KT458 KT458542 c KT458640 KT458730 KT458790 Viola pedatifida 2 2 0 0 0 8 760 7 0 0 a See Kellar et al. 2015 for GenBank accession number b See Aust et al. 2015 for GenBank accession number c DNA region not recovered from Illumina sequencing reads; therefore, coded as missing data in phylogenetic analyses Mitochondrial (mtDNA): Species atp8 cox1 cox3 CYTB matR nad4 nad6 nad7 rps3 KT45879 KT45883 KT45887 KT45891 KT45900 KT45904 KT45909 KT458961 KT459139 Amorpha canescens 1 0 5 6 4 9 4 KT45879 KT45883 KT45887 KT45891 KT45900 KT45905 KT45909 KT458962 KT459140 Amorpha fruticosa 2 1 6 7 5 0 5 KT45879 KT45883 KT45887 KT45891 KT45900 KT45905 KT45909 KT458963 KT459141 Astragalus canadensis 3 2 7 8 6 1 6 KT45879 KT45883 KT45887 KT45891 KT45900 KT45905 KT45909 KT458964 KT459142 Astragalus crassicarpus 4 3 8 9 7 2 7 KT45883 KT45887 KT45892 KT45900 KT45905 KT45909 c KT458965 c Callirhoe involucrata 4 9 0 8 3 8 KT45879 KT45883 KT45888 KT45892 KT45900 KT45905 KT45909 KT458966 KT459143 Cannabis sativa 5 5 0 1 9 4 9 KT45879 KT45883 KT45888 KT45892 KT45901 KT45905 KT45910 KT458967 KT459144 Capsella bursa-pastoris 6 6 1 2 0 5 0 KT45879 KT45883 KT45888 KT45892 KT45901 KT45905 KT45910 KT458968 KT459145 Ceanothus herbaceous 7 7 2 3 1 6 1 KT45879 KT45883 KT45888 KT45892 KT45901 KT45905 KT45910 KT458969 KT459146 Chamaecrista fasciculata 8 8 3 4 2 7 2 KT45883 KT45888 KT45892 KT45901 KT45905 KT45910 c c c Chorispora tenella 9 4 5 3 8 3 KT45879 KT45884 KT45888 KT45892 KT45901 KT45905 KT45910 KT458970 KT459147 Cleome serrulata 9 0 5 6 4 9 4 KT45884 KT45892 KT45901 KT45906 KT45910 b b KT458971 c Comandra umbellata subsp. umbellata 1 7 5 0 5 KT45880 KT45884 KT45888 KT45892 KT45901 KT45906 KT45910 KT458972 KT459148 Croton texensis 0 2 6 8 6 1 6 KT45880 KT45884 KT45888 KT45892 KT45901 KT45906 KT45910 KT458973 KT459149 Desmodium illinoense 1 3 7 9 7 2 7 KT45880 KT45884 KT45888 KT45893 KT45901 KT45906 KT45910 KT458974 KT459150 Euphorbia esula 2 4 8 0 8 3 8 KT45880 KT45884 KT45888 KT45893 KT45901 KT45906 KT45910 KT458975 KT459151 Euphorbia marginata 3 5 9 1 9 4 9 KT45880 KT45884 KT45889 KT45893 KT45902 KT45906 KT45911 KT458976 KT459152 Gaura coccinea 4 6 0 2 0 5 0 KT45880 KT45884 KT45889 KT45893 KT45902 KT45906 KT45911 KT458977 KT459153 Glycyrrhiza lepidota 5 7 1 3 1 6 1 KT45880 KT45884 KT45889 KT45893 KT45902 KT45906 KT45911 KT458978 c Lathyrus decaphyllus 6 8 2 4 2 7 2 KT45880 KT45884 KT45889 KT45893 KT45902 KT45906 KT45911 KT458979 c Lepidium densiflorum 7 9 3 5 3 8 3 KT45880 KT45885 KT45889 KT45893 KT45902 KT45906 KT45911 KT458980 KT459154 Lespedeza capitata 8 0 4 6 4 9 4 KT45880 KT45885 KT45889 KT45893 KT45902 KT45907 KT45911 KT458981 KT459155 Medicago lupulina 9 1 5 7 5 0 5 KT45881 KT45885 KT45889 KT45893 KT45902 KT45907 KT45911 KT458982 KT459156 Medicago sativa 0 2 6 8 6 1 6 KT45881 KT45885 KT45889 KT45893 KT45902 KT45907 KT45911 KT458983 KT459157 Melilotus albus 1 3 7 9 7 2 7 KT45881 KT45885 KT45889 KT45894 KT45902 KT45907 KT45911 KT458984 KT459158 Melilotus officinalis 2 4 8 0 8 3 8 KT45881 KT45885 KT45889 KT45894 KT45902 KT45907 KT45911 KT458985 KT459159 Oenothera nuttallii 3 5 9 1 9 4 9 KT45881 KT45885 KT45890 KT45894 KT45903 KT45907 KT45912 KT458986 KT459160 Oxalis dillenii 4 6 0 2 0 5 0 KT45881 KT45885 KT45890 KT45894 KT45903 KT45907 KT45912 KT458987 KT459161 Oxytropis lambertii 5 7 1 3 1 6 1 KT45881 KT45885 KT45890 KT45894 KT45903 KT45907 KT45912 KT458988 KT459162 Pediomelum argophyllum 6 8 2 4 2 7 2 KT45881 KT45885 KT45890 KT45894 KT45903 KT45907 KT45912 KT458989 KT459163 Pediomelum digitatum 7 9 3 5 3 8 3 KT45881 KT45886 KT45890 KT45894 KT45903 KT45907 KT45912 KT458990 KT459164 Pediomelum esculentum 8 0 4 6 4 9 4 KT45881 KT45886 KT45894 KT45903 KT45908 KT45912 c KT458991 KT459165 Physaria ludoviciana 9 1 7 5 0 5 KT45882 KT45886 KT45890 KT45894 KT45903 KT45908 KT45912 KT458992 KT459166 Polygala alba 0 2 5 8 6 1 6 KT45882 KT45886 KT45890 KT45894 KT45903 KT45908 KT45912 KT458993 KT459167 Prunus virginiana 1 3 6 9 7 2 7 KT45882 KT45886 KT45890 KT45895 KT45903 KT45908 KT45912 KT458994 KT459168 Psoralidium lanceolatum 2 4 7 0 8 3 8 KT45882 KT45886 KT45890 KT45895 KT45903 KT45908 KT45912 KT458995 KT459169 Psoralidium tenuiflorum 3 5 8 1 9 4 9 KT45882 KT45886 KT45890 KT45895 KT45904 KT45908 KT45913 KT458996 KT459170 Salix exigua subsp. interior 4 6 9 2 0 5 0 KT45882 KT45886 KT45891 KT45895 KT45904 KT45908 KT45913 KT458997 KT459171 Senna marilandica 5 7 0 3 1 6 1 KT45886 KT45895 KT45904 KT45908 KT45913 b b KT458998 c Silene antirrhina 8 4 2 7 2 KT45886 KT45895 KT45904 KT45908 KT45913 b b KT458999 c Silene vulgaris 9 5 3 8 3 KT45887 KT45891 KT45895 KT45904 KT45908 KT45913 c c c Sphaeralcea coccinea 0 1 6 4 9 4 KT45882 KT45887 KT45891 KT45895 KT45904 KT45909 KT45913 KT459000 c Vicia americana var. minor 6 1 2 7 5 0 5 KT45882 KT45887 KT45891 KT45895 KT45904 KT45909 KT45913 KT459001 KT459172 Vicia villosa 7 2 3 8 6 1 6 KT45882 KT45887 KT45891 KT45895 KT45904 KT45909 KT45913 KT459002 KT459173 Viola bicolor 8 3 4 9 7 2 7 KT45882 KT45887 KT45891 KT45896 KT45904 KT45909 KT45913 KT459003 KT459174 Viola pedatifida 9 4 5 0 8 3 8 a See Kellar et al. 2015 for GenBank accession number b See Aust et al. 2015 for GenBank accession number c DNA region not recovered from Illumina sequencing reads; therefore, coded as missing data in phylogenetic analyses Nuclear (nrDNA): Species 18S 26S 5.8S KT45921 KT459175 KT459259 Amorpha canescens 7 KT45921 KT459176 KT459260 Amorpha fruticosa 8 KT45921 KT459177 KT459261 Astragalus canadensis 9 KT45922 KT459178 KT459262 Astragalus crassicarpus 0 KT45922 KT459179 KT459263 Callirhoe involucrata 1 KT45922 KT459180 KT459264 Cannabis sativa 2 KT45922 KT459181 KT459265 Capsella bursa-pastoris 3 KT45922 KT459182 KT459266 Ceanothus herbaceous 4 KT45922 KT459183 KT459267 Chamaecrista fasciculata 5 KT45922 KT459184 KT459268 Chorispora tenella 6 KT45922 KT459185 KT459269 Cleome serrulata 7 Comandra umbellata subsp. umbellata b b b KT45922 KT459186 KT459270 Croton texensis 8 KT45922 KT459187 KT459271 Desmodium illinoense 9 KT45923 KT459188 KT459272 Euphorbia esula 0 KT45923 KT459189 KT459273 Euphorbia marginata 1 KT45923 KT459190 KT459274 Gaura coccinea 2 KT45923 KT459191 KT459275 Glycyrrhiza lepidota 3 KT45923 KT459192 KT459276 Lathyrus decaphyllus 4 KT45923 KT459193 KT459277 Lepidium densiflorum 5 KT45923 KT459194 KT459278 Lespedeza capitata 6 KT45923 KT459195 KT459279 Medicago lupulina 7 KT45923 KT459196 KT459280 Medicago sativa 8 KT45923 KT459197 KT459281 Melilotus albus 9 KT45924 KT459198 KT459282 Melilotus officinalis 0 KT45924 KT459199 KT459283 Oenothera nuttallii 1 KT45924 KT459200 KT459284 Oxalis dillenii 2 KT45924 KT459201 KT459285 Oxytropis lambertii 3 KT45924 KT459202 KT459286 Pediomelum argophyllum 4 KT45924 KT459203 KT459287 Pediomelum digitatum 5 KT45924 KT459204 KT459288 Pediomelum esculentum 6 KT45924 KT459205 KT459289 Physaria ludoviciana 7 KT45924 KT459206 KT459290 Polygala alba 8 KT45924 KT459207 KT459291 Prunus virginiana 9 KT45925 KT459208 KT459292 Psoralidium lanceolatum 0 KT45925 KT459209 KT459293 Psoralidium tenuiflorum 1 KT45925 KT459210 KT459294 Salix exigua subsp. interior 2 KT45925 KT459211 KT459295 Senna marilandica 3 Silene antirrhina b b b Silene vulgaris b b b KT45925 KT459212 KT459296 Sphaeralcea coccinea 4 KT45925 KT459213 KT459297 Vicia americana var. minor 5 KT45925 KT459214 KT459298 Vicia villosa 6 KT45925 KT459215 KT459299 Viola bicolor 7 KT45925 KT459216 KT459300 Viola pedatifida 8 a See Kellar et al. 2015 for GenBank accession number b See Aust et al. 2015 for GenBank accession number c DNA region not recovered from Illumina sequencing reads; therefore, coded as missing data in phylogenetic analyses</p>