<p>Supplementary material</p><p>Biological Invasions</p><p>Comparative phylogeography of two colonial ascidians reveals contrasting invasion histories in North America</p><p>Christophe Lejeusne1, 2, Dan Bock1, Thomas W. Therriault3, Hugh J. MacIsaac1, and Melania E. Cristescu1</p><p>1 Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada </p><p>2 Estación Biológica de Doñana - CSIC, Wetland Ecology Department, Avenida Américo Vespucio, s/n, 41092 Sevilla, Spain </p><p>3 Fisheries and Oceans Canada, Pacific Biological Station, Nanaimo, British Columbia, V9T 6N7, Canada </p><p>Corresponding author: Hugh J. MacIsaac, Phone: (519) 253-3000 ext. 3754, Fax: (519) 971-3616, Email: [email protected]</p><p>1 Appendix 1. Pairwise ST values between populations of Botryllus schlosseri. Significant values after Bonferroni corrections (=0.05) are in boldface. 1: Shimoda Bay; 2: Port Lincoln; 3: Deep Bay; 4: French Creek; 5: Ladysmith; 6: Brinnon; 7: Sequim; 8: Port-La-Tour; 9: Ingomar; 10: St Peter's Bay; 11: Louisbourg; 12: Arenys de Mar; 13: Alicante; 14: Sète; 15: Blanes; 16: Estaque; 17: Estartit; 18: Roses; 19: Canet; 20: Cubelles; 21: LaRochelle; 22: Cadaques; 23: Grana; 24: Fornelos. Japan Australia NE Pacific NW Atlantic NE Atlantic and Mediterranean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 1 - 2 0.500 3 0.250 0.250 4 0.691 0.691 0.000 - 5 0.399 0.399 0.031 0.080 6 0.193 0.193 0.113 0.362 0.120 - 7 -0.258 0.400 0.719 0.545 0.386 0.258 8 0.220 0.220 0.321 0.543 0.402 0.180 0.341 9 0.353 0.353 0.508 0.765 0.595 0.268 0.457 -0.072 10 0.713 0.713 1.000 1.000 0.903 0.579 0.669 0.065 0.062 - 11 0.489 0.489 1.000 1.000 0.885 0.474 0.532 -0.126 0.000 0.171 12 0.547 0.547 0.848 0.917 0.860 0.784 0.360 0.640 0.778 0.896 0.880 - 13 0.404 0.404 0.739 0.835 0.773 0.707 0.256 0.605 0.722 0.819 0.796 0.033 14 0.376 0.376 0.562 0.819 0.733 0.638 0.483 0.6240 0.593 0.586 0.422 0.725 0.740 - 15 -0.025 0.402 0.570 0.477 0.413 0.011 0.418 0.519 0.634 0.581 0.246 0.184 0.686 0.025 - 16 0.508 0.508 0.785 0.859 0.808 0.757 0.357 0.662 0.769 0.852 0.835 0.008 0.770 0.269 0.007 17 0.692 0.692 0.902 0.951 0.906 0.838 0.547 0.692 0.818 0.928 0.914 0.360 0.306 0.717 0.447 0.177 18 0.052 0.052 0.309 0.576 0.470 0.384 0.113 0.394 0.383 0.395 0.249 0.233 0.259 0.158 0.277 0.293 0.258 19 0.194 0.194 0.336 0.564 0.473 0.401 0.277 0.430 0.399 0.392 0.268 0.466 0.495 0.015 0.452 0.532 0.469 0.048 20 0.485 0.485 0.717 0.854 0.783 0.699 0.454 0.590 0.674 0.767 0.718 0.552 0.518 0.659 0.478 0.546 0.588 0.309 0.445 21 0.160 0.160 0.393 0.595 0.501 0.419 0.219 0.337 0.390 0.545 0.462 0.426 0.410 0.606 0.312 0.439 0.433 0.293 0.420 0.447 22 0.836 0.836 0.865 0.920 0.895 0.872 0.843 0.854 0.865 0.877 0.861 0.892 0.892 0.363 0.873 0.901 0.891 0.645 0.441 0.873 0.843</p><p>2 - 23 -0.008 0.145 0.307 0.242 0.204 0.088 0.261 0.252 0.263 0.135 0.263 0.275 0.156 0.190 0.314 0.303 0.028 0.085 0.319 0.255 0.565 0.008 24 0.475 0.475 0.563 0.734 0.667 0.613 0.526 0.624 0.594 0.585 0.510 0.665 0.690 0.096 0.664 0.714 0.652 0.346 0.223 0.629 0.615 0.349 0.344</p><p>3 Appendix 2. Pairwise ST values between populations of Botrylloides violaceus. Significant values after Bonferroni corrections (=0.05) are in boldface. NE Pacific Japan NW Atlantic 1 2 3 4 5 6 7 8 9 10 11 12 13 14 1. Deep Bay 2. Ladysmith -0.051 3. Neah Bay 0.027 -0.001 4. French Creek 0.220 0.278 0.386 5. Sequim 0.196 0.252 0.360 0.000 6. Lopez Island 0.354 0.285 0.152 0.776 0.755 7. Brinnon 0.382 0.314 0.173 0.792 0.772 -0.072 8. Shimoda Bay 0.392 0.408 0.462 0.619 0.593 0.722 0.737 9. Aomori 0.767 0.746 0.740 1.000 1.000 0.823 0.836 0.906 10. Cardigan River 0.242 0.302 0.408 0.000 0.000 0.794 0.808 0.449 1.000 11. Methegan 0.110 0.163 0.279 0.000 0.000 0.683 0.707 0.168 1.000 0.000 12. Ingomar 0.142 0.195 0.308 0.000 0.000 0.708 0.730 0.236 1.000 0.000 0.000 13. Lunenburg 0.088 0.141 0.260 0.000 0.000 0.668 0.694 0.125 1.000 0.000 0.000 0.000 14. Savage Harbor 0.220 0.278 0.386 0.000 0.000 0.776 0.792 0.405 1.000 0.000 0.000 0.000 0.000 15. St Peter's Bay 0.228 0.286 0.393 0.000 0.000 0.782 0.797 0.421 1.000 0.000 0.000 0.000 0.000 0.000</p><p>4</p>