<p> SUPPLEMENTARY TABLE LEGENDS</p><p>Table S1: According to AceView (March, 2011), the complete mRNA for variant “a” is 2295 bp long. The pre-message comprises 18 exons from the total of 104 contained within PRKCZ that covers 136.21 kb on the gene. It is exactly encoded by NM_002744. The predicted protein comprises 592 amino acids having a molecular mass of 67.7 kDa. </p><p>Table S2: Primer sequences employed for qPCR validation of PRKCZ variant “a” gene (PRKC--a) expression in cell-lines and primary prostatic tissues.</p><p>Table S3: Tissue-specific primers to analyze PRKCZ gene exon expression in prostate cell-lines and tissues by transcriptome-walking.</p><p>Table S4: Primer pairs for PCR reactions to independently analyze PRKCZ gene expression data obtained by transcriptome-walking using primers defined in Table S3.</p><p>Yao et al SUPPLEMENTARY TABLES SUPPLEMENTARY TABLE S1</p><p>Structure of human PRKCZ variant “a” according to NCBI (March, 2011)</p><p>PRKCZ PRKCZ Length Coordinates on variant “a” Exon included in all variants gene exon # (bp) PRKCZ gene exon # 1 1 a, b, k 203 1-203 2 6 a, k, o, vd 122 4943-5064</p><p>3 8 a, b, o, vd, vj 90 5986-6075 4 9 a, b, k, o, vd, vj 51 9043-9093</p><p> a, b, c, e, g, h, i, j, k, l, n, o, q, s, 5 39 va, ve, vg, vh, vk, vl, vn, vo, vr, vs, 86 84764-84849 vt, vw, vx a, b, c, e, g, h, i, j, k, l, n, p, q, s, 6 43 vc, ve, vg, vh, vi, vk, vl, vo, vr, vs, 132 93712-93843 vt, vw, vx</p><p> a, b, c, f, g, h, i, j, k, l, n, p, q, s, 7 51 vb, vc, ve, vg, vh, vi, vk, vl, vm, 82 95529-95610 vo, vs, vw a, b, c, e, f, g, h, i, j, k, l, n, p, q, s, 8 55 vb, vc, ve, vg, vh, vi, vk, vl, vm, 53 98374-98426 vo, vt, vw, vx</p><p> a, b, c, e, f, g, h, i, j, k, l, vb, vc, 9 69 189 100292-100480 vo</p><p>10 72 a, b, c, e, f, g, h, i, j, k, l, va, vo 98 105497-105594 11 77 a, c, e, f, g, h, i, j, k, l, va, vv 87 119020-119106</p><p>12 81 a, b, c, d, e, f, g, h, i, j, l, vv 136 121557-121692 13 82 a, b, c, d, e, f, g, h, i, j, l, w 88 121803-121890</p><p>14 84 a, b, c, d, e, f, g, h, i, j, l 120 123399-123518 15 85 a, b, c, d, e, f, g, h, i, j, l 80 124256-124335</p><p>16 86 a, b, c, d, e, f, g, h, i, j, l 90 124726-124815 17 90 a, b, c, d, e, f, g, h, i, j, l 116 134085-134200</p><p>18 98 a, d, h, vm 472 134424-134895</p><p>PRKCZ PRKCZ Length Coordinates on variant “vq” gene Also in variants (bp) PRKCZ gene exon # exon #</p><p>1 89 - 279 133922-134200</p><p>Yao et al SUPPLEMENTARY TABLES SUPPLEMENTARY TABLE S2</p><p>Primer sequences to amplify PRCKZ variants and PRCKI</p><p>Cycling conditions for the reaction were: 95⁰C for 15 minutes, then 40 cycles at 94⁰C for 15 seconds, 63⁰C for 30 seconds, plate read and 72⁰C for 30 seconds with a final extension at 72⁰C for 10 minutes</p><p>Amplicon Product Amplification Gene Sequences 5’ → 3’ location Size (bp) Temp (˚C ) (exon-exon)</p><p>PRKC- ζ -a (Fwd-1) ACATGTGTCGTCTGCACCAG 6 - 9 150 60 PRKC- ζ -a (Rev-1) GTGCTCGGGAAAACATGAAT</p><p>PRKC- ζ -a (Fwd-2) CCATGTCCGGGAGAAGAC 9 - 51 288 60 PRKC- ζ -a (Rev-2) TTGGGAAGGCATGACAGAATC</p><p>β-actin (Fwd) AGCACGGCATCGTCACCAACT 3 - 4 180 58 β-actin (Rev) TGGCTGGGGTGTTGAAGGTCT</p><p>Fwd: 5’-CCTTTATGGTCAGGGTTCCA-3’ variant “vq 89ext - 99 224 60 Rev: 5’-GAGCTGAGAAATGCCAGTGTC-3’</p><p>Fwd: 5’-GCTGCAGCGTGAGACTCAG-3’ variant “f” 45 - 51 226 60 Rev: 5’-TCCGAGTCGTCTTTAATGCTG-3’</p><p>Fwd: 5’-TTGTTTCTTGCCCTTTGACC-3’ variant “n” 68 - 69 281 60 Rev: 5’-TTGATGCACCTGTAGCCTTG-3’</p><p>Fwd: 5’-TGGGTTGTCATCCTCAAACA-3’ PRKCI 2 - 17 186 60 Rev: 5’-CATCTGGAGTGAGCTGGACA-3’</p><p>Yao et al SUPPLEMENTARY TABLES SUPPLEMENTARY TABLE S3</p><p>Tissue-specific primers for transcriptome-walking</p><p>Annealing Primer Exon location Sequence temperature designation (March 2011) (Tm - ºC)</p><p>TSP1 98-81 TCCTCGGTGGACAGCAATAAT 59.9 </p><p>TSP2 “ CTCAAAGCCTTCGAACGCTGAC 60.5</p><p>TSP3 “ GGTCGATCCTCTTTATGGCATC 62.2</p><p>TSP4 81-39 GATATTGACTGGGTACAGA 57.0</p><p>TSP5 “ CAGAGAAGCACGTGTTTGAGCA 62.0</p><p>TSP6 “ GACCAGGCATCCAGCAACC 65.0</p><p>TSP7 39-1 CCTGTTAAAGCGCTTGGCT 58.0</p><p>TSP8 “ GTTGGCACGGTACAGCTTC 60.0</p><p>TSP9 “ CTTCCTCCATCTTCTGGCTC 62.0</p><p>TSPr1 89→ TGCTTCTTCTCCAGCTTGG 58.0</p><p>TSPr2 “ GGCATCATCCCTACCT 60.0</p><p>TSPr3 “ TGATTGAGTGTTGCACGCAC 62.0</p><p>TSPr4 89 extension GAGCTGAGAAATGCCCAGTGT 62.0</p><p>TSPr5 ACATGTGGGACGGAGCAG 60.0</p><p>TSPr6 AGAGGGGCACCGACAACTAA 60.0</p><p>Yao et al SUPPLEMENTARY TABLES SUPPLEMENTARY TABLE S4</p><p>Primer pairs for PCR validation of transcriptome-walking data</p><p>Amplicon Expected Products Primer # Sequences location product generated (exon-exon) (bp) (bp)</p><p>AMPLIFICATION OF NOVEL SEQUENCE</p><p>Fwd: 5’-CCTTTATGGTCAGGGTTCCA-3’ variant “vq” 87-89ext 224 224 Rev: 5’-GAGCTGAGAAATGCCAGTGTC-3’</p><p>5’-UPSTREAM AMPLIFICATION</p><p>Fwd: 5’-ACGAGCAAGGGAATGAGAGA-3’ 1,400 1A 87-89ext 428 Rev: 5’-TGGAACCCTGACCATAAAGG-3’ 700</p><p>Fwd: 5’-ACGAGCAAGGGAATGAGAGA-3’ 1B 87-89 718 1,600 Rev: 5’-TGTTTTGCACGCACTTCAAT-3’</p><p>AMPLIFICATION OF EXON 83-EXON 89 BOUNDARY</p><p>Fwd: 5’-GCGCGGAGCTGACCCTTCTC-3’ 2 89-99 357 600 Rev: 5’-CAAGCGTCTGTCCCTGGCCG-3’</p><p>3’-DOWNSTREAM AMPLIFICATION</p><p>Fwd: 5’-AGTGAGCGTGTAGCGTCCT-3’ 3A 99-104 332 554 Rev: 5’-GCCTGTGTTTCTGGGTGTTT-3’ Fwd: 5’-CCACGGAAACAGAACTCGAT-3’ 3B 99-104 374 596 Rev: 5’-GCCTGTGTTTCTGGGTGTTT-3’</p><p>INDEPENDENT ASSESSMENT OF SEQUENCE EXPRESSION</p><p>Fwd: 5’-ACGAGCAAGGGAATGAGAGA-3’ Check 1 87-Intron 49 550 500 Rev: 5’-GCCCTCACAGACAAACTCCT-3’ Fwd: 5’-ACGAGCAAGGGAATGAGAGA-3’ Check 2 87-89 3475 1000 Rev: 5’-GCATATGCGGTGGTTAAGGA-3’</p><p>CONTROLS</p><p>-ve control Fwd: 5’-GCTGCAGCGTGAGACTCAG-3’ 45-55 226 - variant “f” Rev: 5’-TCCGAGTCGTCTTTAATGCTG-3’</p><p>-ve control Fwd: 5’-TTGTTTCTTGCCCTTTGACC-3’ 30-43 281 - variant “n” Rev: 5’-TTGATGCACCTGTAGCCTTG-3’</p><p>Atypical gene Fwd: 5’-TGGGTTGTCATCCTCAAACA-3’ 2-17 186 186 PRKCI Rev: 5’-CATCTGGAGTGAGCTGGACA-3’</p><p>Yao et al SUPPLEMENTARY TABLES</p>