Name Class Date

Use the following link, to view an animation on gene cloning and DNA recombinant technology. Select step through at the menu screen. http://www.sumanasinc.com/webcontent/animations/content/plasmidcloning.html

Slide 1: 1. What is DNA cloning? ______

Slide 2: 2. In the diagram to the right, which letter represents a region where the DNA can be inserted? ______

3. What is region B and what does this region allow the plasmid to do? ______

______

4. What gene is located in region C, what is the function of this gene? ______

Slide 3: 5. What is used to cleave (cut) the DNA? ______6. What are sticky ends? ______

Slide 4: 7. What is the enzyme that forms bonds between the sticky ends of the foreign DNA and the plasmid DNA? ______

8. What are the three regions that are contained on each of the plasmids? ______Name Class Date

Slide 5: 9. Explain what happens through a process called transformation. ______

Slide 6: 10. Why is the bacteria grown in nutrient agar which contains ampicillin? ______

11. What happens to the cells that lack the plasmid? Why? ______

12. What happens to the cells that have the plasmid? Why? ______

Slide 7: 13. What happens to the plasmids when the cell divides? ______

Finding Genes

mRNA Amino Codon Acids UCU SER AGA ARG CCG PRO GGC GLY AUC ILE GUU VAL AAG LYS ACG THR UAG TERM CAA GLN GAU ASP AUG MET UUU PHE UGG TRP Name Class Date mRNA: ______

DNA: ______

G