<p>Electronic Supplementary Materials</p><p>Biomechanical diversity of mating structures among harvestmen species is consistent with a spectrum of precopulatory strategies</p><p>Mercedes M. Burns1#a* and Jeffrey W. Shultz1</p><p>1 BEES Program and Department of Entomology, University of Maryland, College Park, MD, USA</p><p>#a Department of Biology, San Diego State University, San Diego, CA, USA</p><p>1 S1 Table. Sample data.</p><p>2 Species GenBank Molecular Morphological specimen Number of specimens examined accession numbers specimen locality Male Female Penis section</p><p> locality morphology morphology modulus (SX,SY)</p><p>(except SX,SY) Eumesosoma JQ432365, USA: TX: USA: TX: Travis Co., 6 5 3 roeweri JQ432307, JQ432253 Wichita Co. USA: TX: Wichita Co., USA: TX: Williamson Co. </p><p>Hadrobunus JQ432358, USA: FL: USA: FL: Alachua Co. 4 5 1 grandis JQ432300, JQ432249 Alachua Co.</p><p>Hadrobunus JQ432359, USA: TN: USA: TN: Blount Co., 6 5 4 n.sp.1 JQ432301, JQ432250 Sevier Co. USA: WV: Monongalia Co.</p><p>Hadrobunus JQ432360, USA: MD: USA: MD: Garrett Co. 6 5 5 maculosus JQ432302, Howard Co. JQ432361, JQ432362, JQ432303, JQ432304, JQ432251 Hadrobunus JQ432364, USA: MO: USA: KS: Douglas Co. 2 6 3 n.sp. 2 JQ432306, JQ432252 Ozark Co.</p><p>Leiobunum GQ870650, USA: MI: USA: OH: Stark Co. 5 5 2 aldrichi JQ432342, Calhoun Co. JQ432284, GQ872154, GQ870649, JQ432343, JQ432285, GQ872153,</p><p>3 Species GenBank Molecular Morphological specimen Number of specimens examined accession numbers specimen locality Male Female Penis section</p><p> locality morphology morphology modulus (SX,SY)</p><p>(except SX,SY) JQ432344, JQ432286, JQ432238</p><p>Leiobunum JQ432366, JQ432308 USA: FL: USA: VA: Nansemond Co., 2 6 2 bimaculatum Jackson Co. USA: MS: George Co., USA: GA: Toombs Co., USA: GA: Tyton Co. Leiobunum JQ432330, USA: NC: USA: MD: Frederick Co., 2 6 2 bracchiolum JQ432272, JQ432230 Guilford Co. USA: MD: Prince Georges Co.</p><p>Leiobunum GQ870653, USA: MD: USA: NC: Madison Co., 4 8 4 calcar JQ432316, Frederick Co. USA: MD: Garrett Co. JQ432258, GQ872157, JQ432317, JQ432259, JQ432223, JQ432319, JQ432261, GQ870655, JQ432320, JQ432262, GQ872158, JQ432318, JQ432260 Leiobunum JQ432331, USA: MO: USA: MO: Butler Co. 2 5 2 crassipalpe JQ432273, Butler Co. JQ432332, JQ432274, JQ432231</p><p>4 Species GenBank Molecular Morphological specimen Number of specimens examined accession numbers specimen locality Male Female Penis section</p><p> locality morphology morphology modulus (SX,SY)</p><p>(except SX,SY) Leiobunum JQ432321, USA: MD: USA: PA: Bucks Co. 5 7 3 euserratipalpe JQ432263, Montgomery GQ870656, Co. JQ432322, JQ432264, GQ872160 Leiobunum JQ432353, USA: AR: USA: AR: Garland Co. 5 3 3 flavum JQ432295, JQ432245 Garland Co.</p><p>Leiobunum JQ432354, USA: FL: USA: FL: Hernando Co., 5 4 6 formosum JQ432296, Jackson Co. USA: VA: Dickerson Co., JQ432356, USA: VA: Northampton Co. , JQ432298, USA: FL: Liberty Co. JQ432247, JQ432355, JQ432297, JQ432246, JQ432357, JQ432399, JQ432248 Leiobunum GQ870654, USA: VA: USA: VA: Grayson Co. 6 6 3 hoffmani JQ432315, Grayson Co. JQ432257, GQ872159 Leiobunum JQ432345, USA: TN: USA: TN: Hamilton Co., 2 3 2 holtae JQ432287, Cumberland USA: TN: Van Buren Co. JQ432239, Co. JQ432346, JQ432288, JQ432240</p><p>5 Species GenBank Molecular Morphological specimen Number of specimens examined accession numbers specimen locality Male Female Penis section</p><p> locality morphology morphology modulus (SX,SY)</p><p>(except SX,SY) Leiobunum JQ432352, JQ432294 USA: NE: USA: NE: Lancaster Co. 5 5 4 n.sp. 1 Lancaster Co.</p><p>Leiobunum JQ432323, USA: MD: USA: MD: Garrett Co. 6 11 4 nigropalpi JQ432265, Frederick Co. JQ432224, JQ432324, JQ432266, JQ432225, JQ432325, JQ432267, JQ432226 Leiobunum JQ432326, USA: AR: USA: MO: Greene Co., 2 4 1 politum JQ432268, Lawrence Co. USA: MO: Butler Co., JQ432227, USA: AR: Lafayette Co. JQ432327, JQ432269, JQ432228, JQ432328, JQ432270, JQ432229, JQ432329, JQ432271 Leiobunum JQ432370, JQ432312 MEXICO: MEXICO: Puebla, 6 5 2 potosum Tlaxcala, MEXICO: Guerrero Ixtacuixtla</p><p>Leiobunum JQ432340, USA: OK: USA: OK: Comanche Co. 3 6 2 relictum JQ432341, Comanche JQ432282, Co. JQ432283, JQ432237</p><p>6 Species GenBank Molecular Morphological specimen Number of specimens examined accession numbers specimen locality Male Female Penis section</p><p> locality morphology morphology modulus (SX,SY)</p><p>(except SX,SY) Leiobunum JQ432367, MEXICO: MEXICO: Veracruz 5 6 2 royali JQ432309, JQ432254 Veracruz, Xalapa</p><p>Leiobunum JQ432369, JQ432311 USA: AZ: USA: AZ: Cochise Co. 5 5 2 townsendi Cochise Co.</p><p>Leiobunum JQ432339, USA: VA: USA: VA: King George Co., 3 8 2 uxorium JQ432281, Smythe Co. USA: PA: Lancaster Co., JQ432235, USA: MD: Howard Co., JQ432338, USA: PA: Cumberland Co. JQ432280, JQ432236 Leiobunum JQ432348, USA: TN: USA: TN: Sevier Co., 4 4 8 ventricosum JQ432290, Blount Co. USA: TN: Knox Co., JQ432349, USA: KY: Whitley Co. JQ432291, JQ432242, JQ432350, JQ432292, JQ432243 Leiobunum JQ432351, USA: TN: USA: KY: Whitley Co. 4 5 3 verrucosum JQ432293, Cumberland JQ432244, Co. JQ432347, JQ432289, JQ432241 Leiobunum JQ432333, USA: TN: USA: MO: Carter Co., 5 4 3 vittatum JQ432275, Davidson Co. USA: AR: Greene Co., JQ432232, GQ870651, JQ432334, JQ432276, </p><p>7 Species GenBank Molecular Morphological specimen Number of specimens examined accession numbers specimen locality Male Female Penis section</p><p> locality morphology morphology modulus (SX,SY)</p><p>(except SX,SY) GQ872155, JQ432335, JQ432277, JQ432233, JQ432336, JQ432278, JQ432234, GQ870652, JQ432337, JQ432279, GQ872156 Leuronychus JQ432368, USA: AZ: USA: AZ: Cochise Co., 4 3 1 pacificus JQ432310, JQ432253 Cochise Co. USA: CA: San Diego Co., USA: CA: Los Angeles Co., USA: CA: Orange Co. Togwoteeus JQ432371, JQ432313 USA: NM: USA: NV: White Pine Co., 5 2 1 biceps Taos Co. USA: NM: Taos Co.</p><p>8 References</p><p>1 Hedin M, Derkarabetian S, McCormack M, Richart C, Shultz JW. The phylogenetic utility of the</p><p> nuclear protein-coding gene EF-1α for resolving recent divergences in Opiliones, emphasizing intron </p><p> evolution. J Arachnol. 2010; 38: 9-20. </p><p>9</p>