<p>Supplemental </p><p>Table 1 Race designations and avirulence/virulence of P. triticina isolates used in greenhouse tests and field plots for assessing leaf rust resistance in the Tc*3/Americano 25e F6 RIL population.</p><p>Race Avirulence Virulence designation Seedling tests BBBDB Lr1, Lr2a, Lr2c, Lr3a, Lr9, Lr16, Lr24, Lr26, Lr14a, Lr14b, Lr20 Lr3ka, Lr11, Lr17, Lr30, LrB, Lr10, Lr18, Lr21, Lr23, Lr28, Lr39, Lr42 SBDGG Lr3a, Lr9, Lr16, Lr24, Lr26, Lr3ka, Lr11, Lr1, Lr2a, Lr2c, Lr17, Lr10, Lr14b, Lr20, Lr28 Lr30, LrB, Lr14a, Lr18, Lr21, Lr23, Lr39, Lr42</p><p>Adult plant greenhouse and field plot tests MLDSB Lr2a, Lr2c, Lr16, Lr24, Lr26, Lr3ka, Lr11, Lr1, Lr3a, Lr9, Lr17, LrB, Lr10, Lr14a, Lr14b, Lr30, Lr18, Lr21, Lr23, Lr28, Lr39, Lr42 Lr20 TDBGG Lr9, Lr16, Lr26, Lr3ka, Lr11, Lr17, Lr30, LrB, Lr1, Lr2a, Lr2c, Lr3a, Lr24, Lr10, Lr14b, Lr14a, Lr18, Lr20, Lr21, Lr39, Lr42, Lr23, Lr28, MFPSB Lr2a, Lr2c, Lr9, Lr16, Lr11, Lr18, Lr21, Lr23, Lr1, Lr3a, Lr24, Lr26, Lr3ka, Lr17, Lr30, LrB, Lr28, Lr39, Lr42 Lr14a, Lr14b, Lr20 THBJG Lr9, Lr24, Lr3ka, Lr11, Lr17, Lr30, LrB, Lr18, Lr1, Lr2a, Lr2c, Lr3a, Lr16, Lr26, Lr10, Lr21, Lr39, Lr42 Lr14a, Lr14b, Lr20, Lr23, Lr28 TNRJJ Lr16, Lr26, Lr17, LrB, Lr18, Lr21, Lr42 Lr1, Lr2a, Lr2c, Lr3a, Lr9, Lr24, Lr11, Lr3ka, Lr30, Lr10, Lr14a, Lr14b, Lr20, Lr23, Lr28, Lr39 MCRKG Lr2a, Lr2c, Lr9, Lr16, Lr24, Lr3ka, Lr11, Lr1, Lr3a, Lr26, Lr3ka, Lr11, Lr30, Lr10, Lr30, LrB, Lr21, Lr39, Lr42 Lr14a, Lr14b, Lr18, Lr20, Lr23, Lr28 Supplemental </p><p>Table 2 Contingency tablea of adult plant leaf rust infection type distribution of the </p><p>Thatcher*3/Americano 25e F6 RIL population in a greenhouse test.</p><p>Genotype Infection type csLV46+, csLV46+, csLV46-, csLV46-, range QLr.cdl -5BL+ QLr.cdl -5BL - QLr.cdl -5BL + QLr.cdl -5BL - ;12-;23 20 12 12 3 2-3 1 3 6 8 3-4 3 9 1 14 a χ2 = 32.5 p <0.001</p>