<p>Supplementary Table</p><p>Table S1. Oligonucleotides used in the present study</p><p>I. Primers for generating knockout mutations by one-step inactivation1</p><p>∆bcsA::cat 5’- TCAACGCGCGGCTTATCGGGCGTTATCGCGATTATCGTCGT CACGGTGCGGTGTAGGCTGGAGCTGCTTC -3’ 5’- GCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCA GCACACGGAATCATATGAATATCCTCCTTAG -3’ ∆cheA::kan 5’- CAGGTCAGTGTTCCCACAATGCCATCAGCCGAACCGAGGT GACAGCGTGTAGGCTGGAGCTGCTTC -3’ 5’- GCCTGACGGCTCGCTGGCCAGCTTTGTTACATTCGTCATAC CGGTCATATGAATATCCTCCTTAG -3’ ∆cheB::kan 5’- CGCTTCACGCTGCGTGGTCAGACGGTGTATGCGCTAAGTAA GGATTAACGGTGTAGGCTGGAGCTGCTTC -3’ 5’- CGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTA AGATCGACCATTCCGGGGATCCGTCGACC -3’ ∆cheY::kan 5’- ATGGCGGATAAAGAACTTAAATTTTTGGTTGTGGATGACTT TTCCACCATGGTGTAGGCTGGAGCTGCTTC -3’ 5’- CATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTG TTGAGCATATGAATATCCTCCTTAG -3’ ∆csgB::cat 5’- GTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAG GGTGACAACGTGTAGGCTGGAGCTGCTTC -3’ 5’- GTTTCATGTAAAACCCCCATCGGATTGATTTAAAAGTCGAA TGGAAACATATGAATATCCTCCTTAG -3’ ∆fliC::kan 5’- TGGCTGTTTTTGAAAAAAATTCTAAAGGTTGTTTTACGACG TGTAGGCTGGAGCTGCTTC -3’ 5’- AATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAAC CTGCATATGAATATCCTCCTTAG -3’ ∆fliK::kan 5’- GATGGATGAGTTCGCCCAGCGCGCCGCCATGAGGAAACCT GAATGATTCGTGTAGGCTGGAGCTGCTTC -3’ 5’- GAGCGCAACAAAGCGGGGAAAAGACGCGGATTACGGTGCT ACCTCTGACGCATATGAATATCCTCCTTAG -3’ ∆hdfR::cat 5’- GTGTAAAACAAAACATTTAAATCATAACGACAAATAATTTT GCGTGTAGGCTGGAGCTGCTTC -3’ 5’- CCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATT TTCATATGAATATCCTCCTTAG -3’ ∆motA::kan 5’- GCGTTAACGCCTGACGACTGAACATCCTGTCATGGTCAACA GTGGGTGTAGGCTGGAGCTGCTTC -3’ 5’- GGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGAT </p><p>1 Cat/Kan cassette-specific sequences are shown in boldface. TCTTCATATGAATATCCTCCTTAG -3’</p><p>II. Primers for generating the csgD(-756;549)::lacZ reporter fusion2 csgD-EcoRI 5’- CGGAATTCATGTTGTCACCCTGGACCTGGTCG -3’ csgD-BamHI 5’- GACGGATCCCCGCTGATGAACAACGAACGAGCG -3’</p><p>III. Primers for generating the csgD(-756;549)::gfp reporter fusion3 csgD(stop)-gfp(start) 5’- GTCTAATAACGAGATCGCTCGTTCGTTGTTCATCAGCGGG taaGAAGGAGATATACATatgAGTAAAGGAG -3’ lacZ(u1040)-cat' 5’- CCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTC CCCGTACCCTAGGTCCTATTCC -3’</p><p>2 Numbers in the designation of the fusion indicate the numbers of nucleotides upstream and downstream of the csgD start codon that are present in the fusion construct; restriction sites in the oligonucleotides are indicated by italics; 3 The stop codon that terminates csgD translation and the start codon for gfp are indicated by italics.</p>