<p>Microbiology Lab Report #3 – Spring 2015</p><p>Name:</p><p>In this report you will draw and describe the appearance of growth on/in each broth and slant you inoculated. Broth cultures should be observed both before and after mixing. </p><p>SLANTS: Growth on your slants should be described by color and amount (abundant, sparse, absent). Draw and describe your observations in the appropriate boxes in the table below. </p><p>BROTHS </p><p>The pattern of growth in a broth should be observed before any mixing, and can be described (as shown in figure 2-37 on page 73) as:</p><p>Flocculent Sediment Ring Pellicle Uniform fine turbidity</p><p>Amount of growth in broth can be described by its degree of cloudiness (turbidity), using a qualitative scoring system:</p><p>+++ means ‘very turbid’ ++ means ‘somewhat turbid’ + means ‘barely turbid’ 0 means not turbid</p><p>Observe the amount of growth before, and then after complete mixing.</p><p>Fill out the table below for each of your inoculated tubes., and then answer the questions that follow.</p><p>Medium Inoculated Source Nutrient Agar Slant Nutrient Broth Appearance S. epidermidis NA Slant</p><p>S. epidermidis NB</p><p>S. epidermidis NA Plate</p><p>NOT DONE Questions:</p><p>1. Considering the cultures used to inoculate each medium in this exercise, how many different microbial types should you expect to see in/on each medium?</p><p>2. You were asked to describe differences in the appearance of growth in each culture, if present. In which medium was this the most difficult to determine? What made this difficult?</p><p>3. Did you notice a difference in density (turbidity) of growth in NB tubes inoculated from NB versus NA slants? Whether or not you did, suggest a possible reason why a difference might occur.</p><p>4. Match the following: ______Flocculent a. Evenly cloudy throughout</p><p>______Sediment b. Growth at top around the edge</p><p>______Ring c. Growth on the bottom</p><p>______Pellicle d. Membrane at the top</p><p>______Uniform fine turbidity e. Suspended chunks or pieces</p>