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Supplemental Table 1 107 Candidate Primer Pairs Which Cross-Amplified in Lamprotula Leai

Supplemental Table 1 107 Candidate Primer Pairs Which Cross-Amplified in Lamprotula Leai

<p>Supplemental Table 1 107 candidate primer pairs which cross-amplified in Lamprotula leai </p><p>Table S1 107 candidate primer pairs which cross-amplified in Lamprotula leai Order GenBank Primer sequences(5'—3') Repeat mode Annealing Size range Reference accession temperature no./locus 1 Cgi- 1 F:TTGCAGGAAGCAAGAGATGA —b 57 30- 80 Launey & Hedgecock R:CTTGTTAACTGCCGGTGAGG 2001 2 Cgi- 10 F:TGCACCAATTTGAGATGTGA —b 50 70- 600 Launey & Hedgecock R:ACTGAGTTTGAAAATGTCACCG 2001 3 Cgi- 18 F:TCCATGTTTACTGCTACTTTTGG —b 50 50- 100 Launey & Hedgecock R:AAATGCTGTGCAGAGAAGCC 2001 4 Cgi- 22 F:GGAAGAGGAATAGTCTACTTATGC —b 43 100- 240 Launey & Hedgecock R:GTCAGACGTTCCTAACTCTTC 2001 5 Cgi- 24 F:CAGAGAGCCGGACTATTTC —b 45 100- 550 Launey & Hedgecock R:GCTCTTTGACACTATGCCGA 2001 6 Cgi- 25 F:CATCAGGGGTAAATTAAAGTAAGC (GT)35 55 90-120 Magoulas et al 1998 R:CCACAGACGATTTCATATATCCTG 7 Cgi- 26 F:ATATGTAATGATTACGAAACTC (GT)18 55 90-600 Magoulas et al 1998 R:GTATGAGATTTGGTTCCACC</p><p>8 Cgi- 29 F:TCAAACCATCTGCTCGTCTACG (GA)n>30 60 90-600 Huvet et al 2000 R:TCCGAAAATCCAGGAATACCGG</p><p>9 Cgi- 30 F:TCGTCACCTCCCTCTCAGAG (GA)17 60 60-600 Sekino et al 2003 R:GCTGTATTTCCATCAATTCGAG 10 Cgi- 32 F:TTGCAGGAAGCAAGAGATGA (TC)11TA(TC)8 60 40- 620 Sekino et al 2003 R:CTTGTTAACTGCCGGTGAGG</p><p>11 MP7 F:AGCTGGAGTAAAAATGAAACGTAATGT (AG)15 51 160–190 Xu et al 2011 R:AGTATTTCACACAATGTTTGTCTGTCC</p><p>12 MP15 F:TGATGAGGAGATGAAGTCGGTCAG (CA)5 52 147–200 Xu et al 2011 R:GTAGACGGAAGGATGCCAAGAAGA</p><p>13 MP17 F:CCTAAATGAGGGTGGTCGCAGTA (CA)11 53 155–250 Xu et al 2011 R:CGGTTTCAATGGACCTAAACGAG a 14 MP19 F:AACTGTGGTGTCCCAACGACCTCTT (GAT)10 53 220–285 Xu et al 2011 R:TTGTGGAATGTCATTGCCAGTGCTA</p><p>15 MP42 F:TACAATCAATATCAAAACGGAACTA (CT)15 50 235–370 Xu et al 2011 R:TCGAGAAAAATCTGTTGTTATTATG</p><p>16 MP43 F:GTTTTGGCATAATCGGAAAGAGGGAC (AG)18 53 215–262 Xu et al 2011 R:ACCTTATCACATTTACGCCGAAGCAC</p><p>17 MP47 F:GCAGGAAACTAGCATTTTCGGCA (CT)10 53 125–147 Xu et al 2011 R:ACTCTGCCTCTGCACACACTGCA</p><p>18 AP05 F:TTCCAAGTCACACAGGTCTTGACAC (TG)7C(GT)8 50 150–204 Xu et al 2011 R:TGTACAGAAAACGAGTTGATGCACA</p><p>19 AP10 F:CTTTCTCCCAAGGGCAAAT (CT)6…(AG)5…(AG)15 48 180–212 Xu et al 2011 R:TCGGTCTCCGTAGAAGCCT</p><p>20 AP24 F:GTTATGAAAAACATCCCGCACTAG (AC)17 51 190–230 Xu et al 2011 R:ATATTCAGCACTCATCTGCCGATA</p><p>21 AP42 F:KKVRVRVCTCTCTCTCTCT (TG)20…(TG)6…(AG)6 57 190–214 Xu et al 2011 R:AGCTGCTAACGTCTATGAAATG</p><p>22 AP44 F:KKVRVRVCTCTCTCTCTCT (TG)5C(GT)5(GA)6 57 188–212 Xu et al 2011 R:AAATGGCGATAAACCCTACAAC 23 APSF2 F:KKVRVRVCTCTCTCTCTCT (GA)9GC(GA)8GG(GA)6 57 364–410 Xu et al 2011 R:GACGATGCTATTTGTGATACTT a 24 APS28 F:ATGGGCTAAACTCGATGAA (AG)19 54 132–166 Xu et al 2011 R:GGTCGGTTCGACTTTGAAA</p><p>25 APS31 F:TCTGGACTTATGAATGATGGTACA (AG)6…(GA)11 54 100–112 Xu et al 2011 R:ACTTTAAAACTCTCTGGTCGGTTT a 26 APS45 F:AGAAGGATGAGGAGGATAAAAGCC (CATA)8 54 234–276 Xu et al 2011 R:AACCGACCAACGGAAAGGGTAAAA</p><p>27 APSF29 F:KKVRVRVCTCTCTCTCTCT (AG)17 57 214–278 Xu et al 2011 R:CTGGACTTATGAATGATGGTAA</p><p>28 APSF36 F:KKVRVRVCTCTCTCTCTCT (TG)7(AG)6 57 242–272 Xu et al 2011 R:ACATGCAATATGCAAAGTTCGA</p><p>29 Hcu01 F:CCACGCACAAATGAAACTCAA (GT)9 55 356–374 Li et al 2007 R:TGGGACAGCAGTGCATTACAG</p><p>30 Hcu02 F:ACACAGACACTCACAGACACA (CT)11 55 212–236 Li et al 2007 R:CACGCCAGCATAAAAACT</p><p>31 Hcu03 F:AACGCTACATAGACAGACAGATATG (GACA)17 55 128–148 Li et al 2007 R:CAGCGTGACTCACTGTGACA</p><p>32 Hcu06 F:AGCAAGGGGAAAATTGAGATG (GA)9 55 108–204 Li et al 2007 R:TGATGAGGGCTCGTATAGATTTAC</p><p>33 Hcu09 F:GCTCGTCGTGATCAAAATGAAA (AG)14 60 130–172 Li et al 2007 R:CAATATGAAAAGAAATGGCAAGAA</p><p>34 Hcu10 F:GTGTTTGGTGAAAATGTGGAGAGT (GT)14 60 160–172 Li et al 2007 R:TGTTAAAGCACCATTCGATACAAA</p><p>35 Hcu15 F:TTAATGCCCACACCTTTTGTTTC (GT)35 60 228–292 Li et al 2007 R:CTTGGGGAGATGCACTCTGG 36 Hcu17 F:AGAAAATAAAACTAAACGGGGATTTA (GATA)9 55 104–216 Li et al 2007 R:CTCCGAGAATACTGAACATGACTTTAT</p><p>37 Hcu18 F:TTGGCGCTCGGCATTTGTTAG (CA)9 55 281–327 Li et al 2007 R:AAGCTGCCGTTCAATCAATGTCA</p><p>38 Hcu19 F:ATCCGCCGAATTATCAAACCT (AC)8 55 154–166 Li et al 2007 R:TTTCGTCCTGTCTGCCACTTTA a 39 HCM01 F:GGCACCATGTGGACATTCTA (ACAT)5G(CATA)7 62 136–178 Bai et al 2009 R:ACTCCCTAGGGCGCTAACAT a 40 HCM02 F:ACTCCAAATTTCAGCGCAGT (TAA)10 60 191–278 Bai et al 2009 R:TTTTTCAGAAATGGGAGGATTT a 41 HCM08 F:TCCCTTGCCTATCACATCAA (TA)11 59 145–185 Bai et al 2009 R:CAACCTGCTATGAGTAATGTGGA</p><p>42 HCM09 F:CTTTGGCGGGTCACTGTACT (TAA)15(GAA)8 58 235–349 Bai et al 2009 R:TGCGTTCGTTGTCTCTTGTT a 43 HCM11 F:GCAGTTACATTTGCCACGTTT (CTA)5ATAATAAT(AA 56 232–274 Bai et al 2009</p><p>R:TTGAGTCAGAAATGGGTGGA G)7</p><p>44 HCM12 F:CGTTAACTGACGCTGCGATA (CA)18 48 149–207 Bai et al 2009 R:TCCCTTACATGAATCTGGTCA a 45 HCM13 F:ACGTTGACGAATCAGACGTG (TA)37 48 135–172 Bai et al 2009 R:GAGACGGAATGTTGCTATGC a 46 HCM14 F:GCAGTTACATTTGCCACGTTT (TAA)5(GAA)6 59 224–264 Bai et al 2009 R:TTGAGTCAGAAATGGGTGGA</p><p>47 HCM17 F:TTTTTCAGAAATGGGTGGATTT (TTC)7TTATTATTAT(TA 50 232–291 Bai et al 2009</p><p>R:GCAGTTACATTTGCCACGTTT G)5</p><p>48 HCM19 F:GCCCACTTGACAGTGAAACA (TC)12 48 192–235 Bai et al 2009 R:CGTTTTTGGCTTTGTCCCTA 49 HCM25 F:GCCAATTGCGTGAGCTACTT (AT)24 48 120–136 Bai et al 2009 R:GCCGCAGTGTGGATTTATTT</p><p>50 HCM28 F:GAAGGGAGCGCAACTTTTCT (AC)13 50 295–377 Bai et al 2009 R:AACTGAAGCCGTCTTCCAAA a 51 HCM29 F:AATGAGTGAGGAGGCAGGAC (TA)15 59 172–204 Bai et al 2009 R:GGGTGGGGGATGTTTTTAAT</p><p>52 HCM31 F:GCCTTAGCGGTGTTCAAGAG (AC)10 62 256–320 Bai et al 2009 R:ATGGCACAAGCCGAAAATAA</p><p>53 Ecap1 F:TGCATCATATGAAATGTGTTCG (AG)17(GT)17 55 146-190 Jones et al 2004 R:TCAGCATATTTCAAAGCAAACA</p><p>54 Ecap2 F:ATCCTCAGGTTGGTGGTCAG (GT)14 60 87-123 Jones et al 2004 R:TTTGAAAACCTTGTGATTGGC</p><p>55 Ecap4 F:ATGCCCCAGTGCTAGACATT (CA)10 55 88-280 Jones et al 2004 R:AGAACAAAACACCCGTGTCC</p><p>56 Ecap8 F:TGCAGACATCGTAGCGATATG (CA)15 55 102-201 Jones et al 2004 R:ATTTCCAGTTGCAAGTCTCATT</p><p>57 Ecap9 F:AAAAAGGTGTGGAGAGAGATGG (GT)18 55 98-238 Jones et al 2004 R:CCACTCTGCAGATATCGTATCG</p><p>58 Ecap10 F:ACACTGCAGACATCGTAGCG (AC)20 52 118-238 Jones et al 2004 R:TCACATACTTTGGGGACTTTCA</p><p>59 MarMa5167 F:AAAGGTCGTTCTTCACATCA (CAA)3CAGCAA(CA)14 55 125-238 Geist et al 2003 R:GCAACCAAGACCCGAATTA</p><p>60 MarMa4726 F:CTTGTGTCCAATCCCTTTGC (TC)15…(CA)4 55 145-308 Geist et al 2003 R:GTCAACCCATCCCAAACAAT</p><p>61 MarMa4315 F:TCATTGTGTCATTAGCAATTTTTG (CTAT)3(CT)12(AT)15AA 55 147-274 Geist et al 2003 R:CCATTGCACTTAGCTGGAAA A(CA)7CT(AT)3</p><p>62 MarMa4277 F:TGTCGGAAATCTTAGCTTGG (CT)20(CA)16 55 108-330 Geist et al 2003 R:GGCGAAATTGAATGCTCTAA</p><p>63 MarMa3116 F:TTAGCCGCTATTACAAAACG (GT)3…(GT)3… 55 118-223 Geist et al 2003</p><p>R:AGAATGAAACATTCGACAGC (CA)5CG(CA)15 c 64 ZJUpy002 F:CAGGAAGAAACACACA (AC)19AG(AC)8 54.4 260-322 Zhu et al 2010 R:TCAAGTGAGGGTGTG c 65 ZJUpy003 F:TCACGCTATGTATGT (TATG)6(TG)13 53.2 227-277 Zhu et al 2010 R:ACTGTACAACACACA c 66 ZJUpy005 F:CTCTCTCACTCTCTC (TC)18TT(AC)18TCTC(A 53.2 199-252 Zhu et al 2010</p><p>R:TATCAAGCAAGTGTG C)4 c 67 ZJUpy006 F:AAACCGTCTTTGTG (GT)17 51.0 195-229 Zhu et al 2010 R:ATCTTCGTACACACA c 68 ZJUpy007 F:AGAACTATACGTGTG (GT)4TT(GT)12 49.9 195-217 Zhu et al 2010 R:TTCCAAATCACACAC c 69 ZJUpy008 F:ACAATGAATATAAA (GTT)5 52.0 182-233 Zhu et al 2010 R:TAAACCCCACAA c 70 ZJUpy013 F:TGCACACTTGAGAGAAG (GAA)6 50.6 271-307 Zhu et al 2010 R:CCACTCCATCTTCTTCG c 71 ZJUpy018 F:CTATCTGTCTATCTGTCTGTCT (CCGT)5(CCAT)4(CTAT) 51.8 303-369 Zhu et al 2010</p><p>R:AGATGGATGGATGGATGGA 4(GT)21 c 72 ZJUpy019 F:GTTGTGCGAATAACG (GA)3AA(GA)3GG(GA)5 58.0 289-313 Zhu et al 2010 R:CTCTCTCCCTCTCTC c 73 ZJUpy023 F:ACATTGCTTT (CA)19 55.0 169-203 Zhu et al 2010 R:TGCATATATATGTG c 74 ZJUpy030 F:TTCAAGTGTGTG (GT)19 48.2 203-245 Zhu et al 2010 R:CAAATCCTCCAC</p><p>75 GQ302634 F:CATCTCTCATTCTGTGGTG (TG)19 52 300-318 Zhu et al 2010 R:GCTTCCTAAAAAGACTCAAAT a 76 GQ302635 F:CTGCTACACAAGAAACAATGACAT (TG)20 52 222-299 Xu et al 2010 R:AGCCACACACACGCACGCA a 77 GQ302636 F:ATAGCGATCATTCTCACTGCAC (TG)18TA(TG)2 55 67-139 Xu et al 2010 R:GCTGACACCTGTACCCACTCT</p><p>78 GQ302637 F:GCTTGTTATCACTAATCATCATCCTA (AC)6TC(AC)10 54 141 Xu et al 2010 R:GTAGTAGCAAAGGGAATGAAA</p><p>79 GQ302638 F:GACAGACTTATGTGGGGAAACC (AC)30 52 200 Xu et al 2010 R:AAGGCTAGGATGGTCAGGATA</p><p>80 GQ302639 F:AGCCTCCTCCATTATACCTTCC (CA)3GA(CA)2TA(CA)12 52 158-179 Xu et al 2010 R:GATTTGCCAATGTGTTTGTATG</p><p>81 GQ302640 F:TGGAACAACCTCCTTTGATAA (CA)12A(CA)8 52 209 Xu et al 2010 R:TGCCAATGTGTGTATGTAGAGTA</p><p>82 GQ302641 F:GCATTGTATAGCCAGGATTGT (CA)20 52 235-266 Xu et al 2010 R:TGTCAGCAGGAGATGGATGG</p><p>83 GQ302642 F:TACATGCTCCCATTTATTTGC (CA)16 54 197 Xu et al 2010 R:ACTTCATCCACCCACAATAA</p><p>84 GQ302643 F:AGGTTGGTTTTAGGACTGGTT (CA)16 54 242 Xu et al 2010 R:CTACAAAGTGACGACGATTACA</p><p>85 GQ302644 F:GCTGGTGGGACCCCTACTA (CA)15 56 154 Xu et al 2010 R:CTTGGGTCTCTTGTTATCACTG</p><p>86 GQ302645 F:TCAGGATAATGTAACTGGGTG (GT)7GA(GT)10 55 150-175 Xu et al 2010 R:GTGTGAATAGTAGATTATGGGTT a 87 GQ302646 F:TCAGCCAGAGTGACCTAAACCT (CA)17 52 200-333 Xu et al 2010 R:TAACCCGCCAGTCTTACTTGAA a 88 GQ302647 F:GAGTCCTGAGTAAGGGAACGAG (CA)3TA(CA)23 52 107-176 Xu et al 2010 R:GAGTTCAAAACAAACAGTCCCT</p><p>89 GQ302648 F:TTTTCTTTTCCGCTTTCATAC (CA)27(TACA)8 55 240-268 Xu et al 2010 R:CAGAGGTGAGATGGCAGTGAT</p><p>90 GQ302649 F:AACTCGCTTGAAAGACATTGC (CA)16 52 209 Xu et al 2010 R:TGGAGCGCGTTTCGCAAAGTATGA</p><p>91 GQ302650 F:CACCCTCATTGCCAAACTA (CA)18 55 23 Xu et al 2010 R:TCACGCTTTAGGCAACACT</p><p>92 GQ302651 F:ATCCTCAACATCGCCCATAC (CA)20 52 164-180 Xu et al 2010 R:TACTATACTCACATGTGCAA</p><p>93 GQ302652 F:GTCCTTTCAGGGCAGCAATA (CA)20 54 174 Xu et al 2010 R:TCTGTTCCAAGACGAATAAGC</p><p>94 GQ302653 F:ATTTGTTGCCCGCCACTTT (CA)21 52 102-115 Xu et al 2010 R:ATATCCCCAATTCCACCTTC</p><p>95 GQ302654 F:GAATCAATGCGAGTAAAGTGG (CA)28 52 231-252 Xu et al 2010 R:AGAAACAAACCGCTGAAAGA</p><p>96 GQ302655 F:TATCCACCAGCCTTTCGTCC (CA)23CT(CA)2 54 212 Xu et al 2010 R:AATAGGGGTCACCTTTCTACAA a 97 GQ302656 F:ATTCCTACATACTAACTGACCG (CA)81 52 157-259 Xu et al 2010 R:GCCCCTCACCTGAACACTC</p><p>98 GQ302657 F:ACTGTAAAATGGACCCGTGT (CT)26(CA)15 55 300-328 Xu et al 2010 R:CATAGAACTAAAGCCCGAAGA</p><p>99 GQ302658 F:ACGCAAGCAAAGGAGAATG (TC)21;(AC)12 52 113-132 Xu et al 2010 R:GAATAGGCTCCGTGTCTTT 100 GQ302659 F:AATAAGCCCAAGAGGACGACA (TC)21 55 300-324 Xu et al 2010 R:TGCGGTTACAGATGAAAGAC</p><p>101 GQ302660 F:GAGTGGGGGAAATAGCCA (CA)44 54 246 Xu et al 2010 R:TAATATCAGAAGGCGAGCGA</p><p>102 GQ302661 F:ACAAGCCCCAAAGCTCAA (CA)19 54 196 Xu et al 2010 R:GAAGACATATAATGGGCAAAG</p><p>103 GQ302662 F:GGAAAGACAAATACGCGC (CA)11 52 150 Xu et al 2010 R:ATGCGTACACCTTCAAACA</p><p>104 GQ302663 F:GACGGTTGCGTTGGAACA (CA)15 52 159 Xu et al 2010 R:AGCGAGCCTACCTGATGGA</p><p>105 GQ302664 F:GGACCATGTTCGGAGAAACG (CA)81 52 367 Xu et al 2010 R:CAGAAGGAAGTGGAGGTGGC</p><p>106 GQ302665 F:AGGAAAACAATGCTTCAGAT (CA)20 52 94-109 Xu et al 2010 R:AGTCAGTGCCCTATGTGTAAT</p><p>107 GQ302667 F:TCAGGGCATAAGATAAGTAGAG (CA)2CT(CA)31 56 189 Xu et al 2010 R:CGATTTGCCAATGTGTGTATGTA a Polymorphic primers successfully amplified in Lamprotula leai b Without information in the GenBank c Primers designed based on products sequence from GenBank using Primer Premier 5.0</p>

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