<p>Table S4. QTL data for five GS traits detected by QTL Cartographer version 2.5 in VH population from three environments (DEL: Delhi, GWL: Gwalior and LEH)</p><p>Source of QTL detected Intra- position at Additive trait Environment Flanking Traits by LG LOD score R2 (%)a population peak LOD effect enhancing s positions Cartographer meta-QTL allele Prpyl-A9-1 A9 68.9 2.6 -2.7 1.9 Heera GWL E-Prpyl-A9-1 68.6 - 69.6 Prpyl-B4-1 B4 62.2 33.1 14.0 50.2 Varuna DEL Prpyl-B4-2 B4 62.2 34.7 12.4 51.8 Varuna GWL S-Prpyl-B4-1 61.5 - 63.3 Prpyl-B4-3 B4 63.2 23.0 11.7 42.2 Varuna LEH Percent Prpyl-B8-1 B8 31.5 11.9 -6.4 11.0 Heera DEL propyls (Prpyl) Prpyl-B8-2 B8 32.2 11.0 -6.5 14.0 Heera LEH S-Prpyl-B8-1 30.7 - 33.2 Prpyl-B8-3 B8 43.3 14.5 -6.5 13.5 Heera GWL Prpyl-B8-4 B8 120.0 7.8 -4.9 6.5 Heera DEL Prpyl-B8-5 B8 128.1 3.6 -3.2 2.6 Heera GWL S-Prpyl-B8-2 118.8 - 130.5 Prpyl-B8-6 B8 128.1 6.2 -4.6 7.0 Heera LEH Total 10 ------4 (3S, 1E) - Btyl-A3-1 A3 27.9 7.8 5.9 12.2 Varuna LEH Btyl-A3-2 A3 28.0 13.5 6.9 17.2 Varuna GWL S-Btyl-A3-1 27.1 - 35.2 Btyl-A3-3 A3 33.7 9.3 6.7 13.0 Varuna DEL Btyl-A5-1 A5 45.7 3.4 -3.8 4.3 Heera DEL E-Btyl-A5-1 44.3 - 46.4 Btyl-B4-1 B4 62.2 21.5 -12.4 43.9 Heera DEL Percent Butyls Btyl-B4-2 B4 62.2 26.3 -11.3 45.3 Heera GWL S-Btyl-B4-1 61.5 - 62.9 (Btyl) Btyl-B4-3 B4 62.2 22.1 -11.5 45.2 Heera LEH Btyl-B8-1 B8 31.3 3.5 3.7 4.6 Varuna LEH Btyl-B8-2 B8 31.5 2.6 3.4 3.3 Varuna DEL S-Btyl-B8-1 28.7 - 45.5 Btyl-B8-3 B8 43.3 5.6 4.1 5.8 Varuna GWL Btyl-B8-4 B8 114.9 3.2 3.0 3.2 Varuna GWL E-Btyl-B8-1 113.5 - 117.7 Total 11 ------5 (3S, 2E) - Source of QTL detected Intra- position at Additive trait Environment Flanking Traits by LG LOD score R2 (%)a population peak LOD effect enhancing s positions Cartographer meta-QTL allele Ptyl-A1-1 A1 45.4 3.3 -1.7 4.4 Heera LEH S-Ptyl-A1-1 44.7 - 49.1 Ptyl-A1-2 A1 46.4 4.1 -2.0 5.1 Heera GWL Ptyl-A3-1 A3 27.7 21.9 -5.5 46.1 Heera LEH Ptyl-A3-2 A3 28.0 26.2 -6.2 50.9 Heera GWL S-Ptyl-A3-1 27.2 - 36.4 Percent Ptyl-A3-3 A3 35.2 21.9 -6.7 50.5 Heera DEL pentyls (Ptyl) Ptyl-A6-1 A6 56.4 2.5 1.6 2.9 Varuna DEL E-Ptyl-A6-1 52.8 - 58.0 Ptyl-B8-1 B8 31.5 5.4 2.6 7.4 Varuna DEL Ptyl-B8-2 B8 32.2 11.4 3.3 15.1 Varuna GWL S-Ptyl-B8-1 31.3 - 46.8 Ptyl-B8-3 B8 42.7 9.0 3.1 15.6 Varuna LEH Total 9 ------4 (3S, 1E) Ali-A1-1 A1 60.4 3.3 -5.5 4.1 Heera DEL E-Ali-A1-1 60.0 - 61.4 Ali-A3-1 A3 26.8 16.4 13.3 27.8 Varuna DEL Ali-A3-2 A3 27.7 11.7 10.2 17.9 Varuna GWL S-Ali-A3-1 26.6 - 28.1 Ali-A3-3 A3 27.7 10.7 10.0 19.8 Varuna LEH Ali-A7-1 A7 21.0 3.3 5.0 4.1 Varuna DEL E-Ali-A7-1 19.5 - 31.4 Ali-A9-1 A9 14.7 13.1 11.9 21.2 Varuna DEL</p><p>Total Ali-A9-2 A9 16.9 13.0 10.6 23.5 Varuna LEH S-Ali-A9-1 13.3 - 21.2 aliphatics (Ali) Ali-A9-3 A9 18.0 10.6 9.5 15.8 Varuna GWL Ali-A9-4 A9 134.1 3.8 5.3 5.0 Varuna GWL E-Ali-A9-1 125.2 - 136.5 Ali-A10-1 A10 53.2 3.3 -5.1 4.6 Heera LEH E-Ali-A10-1 50.9 - 54.4 Ali-B3-1 B3 84.2 8.3 8.2 13.1 Varuna LEH E-Ali-B3-1 83.8 - 84.5 Ali-B3-2 B3 125.2 5.2 6.9 6.8 Varuna DEL S-Ali-B3-1 123.2 -127.2 Ali-B3-3 B3 125.2 4.9 6.1 6.3 Varuna GWL Ali-B8-1 B8 56.3 3.5 4.9 4.4 Varuna GWL E-Ali-B8-1 52.0 - 61.7 Total 14 ------9 (3S, 6E) - Source of QTL detected Intra- position at Additive trait Environment Flanking Traits by LG LOD score R2 (%)a population peak LOD effect enhancing s positions Cartographer meta-QTL allele ToGsl-A1-1 A1 60.4 3.4 -5.7 4.4 Heera DEL E-ToGsl-A1-1 60.0 - 61.4 ToGsl-A3-1 A3 26.3 14.7 11.6 27.5 Varuna LEH ToGsl-A3-2 A3 26.8 15.7 13.1 26.9 Varuna DEL S-ToGsl-A3-1 26.0 - 30.0 ToGsl-A3-3 A3 27.7 11.2 10.0 17.1 Varuna GWL ToGsl-A4-1 A4 0.0 2.8 -4.3 4.0 Heera LEH E-ToGsl-A4-1 0.0 - 3.0 ToGsl-A7-1 A7 21.0 3.3 5.1 4.2 Varuna DEL E-ToGsl-A7-1 19.5 - 31.4 Total ToGsl-A9-1 A9 14.7 12.8 11.8 21.0 Varuna DEL glucosinolates (ToGsl) ToGsl-A9-2 A9 14.7 13.1 10.8 24.7 Varuna LEH S-ToGsl-A9-1 13.2 - 21.3 ToGsl-A9-3 A9 18.0 10.6 9.5 15.9 Varuna GWL ToGsl-A9-4 A9 134.1 3.9 5.3 5.1 Varuna GWL E-ToGsl-A9-1 125.3 - 136.5 ToGsl-B3-1 B3 91.2 4.9 6.0 7.2 Varuna LEH E-ToGsl-B3-1 91.0 - 93.5 ToGsl-B3-2 B3 125.2 5.3 7.0 7.1 Varuna DEL S-ToGsl-B3-1 123.2 - 127.2 ToGsl-B3-3 B3 125.2 4.9 6.2 6.5 Varuna GWL ToGsl-B8-1 B8 56.3 3.5 4.9 4.5 Varuna GWL E-ToGsl-B8-1 52.0 - 61.7 Total 14 ------9 (3S, 6E) -</p><p> aMajor QTL (R2 ≥ 10.0%) are highlighted </p>