<p> Aimee Rebecca Paskins MRes BSc MRSB [email protected] 07814720374</p><p>Education</p><p>October 2013- October 2016: PhD Molecular Biology and Biochemistry - Sheffield Hallam University</p><p>I am currently completing my project investigating the role of a number of factors on both the conformation of the α-Synuclein protein, and its subsequent aggregation, under the supervision of Dr David Smith and Dr Caroline Dalton.</p><p>I have utilised a number of techniques including electrospray ionisation - ion mobility spectrometry - mass spectrometry (ESI-IMS-MS), inductively coupled plasma - mass spectrometry/ mass spectrometry imaging (ICP-MS/MSI), matrix assisted laser desorption - mass spectrometry imaging (MALDI-MSI), protein synthesis, cell culture and immunocytochemistry, thioflavin-t assays, toxicity assays, dot blotting and enzyme-linked immunosorbent assays (ELISA).</p><p>2011-2012: MRes Oncology – The University of Manchester – Merit</p><p>Based at the Christie Hospital NHS Foundation Trust and the Paterson Institute for Cancer Research, Manchester</p><p>2005-2008: BSc (Hons) Human Biosciences – Sheffield Hallam University 2:1</p><p>Employment History</p><p>October 2014 - Present: Laboratory Demonstrator - Sheffield Hallam University, Sheffield</p><p>Demonstrating and teaching in undergraduate and masters level laboratory classes.</p><p>March 2013 - August 2013: Assistant Research Technician - Sheffield Hallam University, Sheffield</p><p>Design, optimisation and utilisation of Liquid Chromatography - Mass Spectrometry (LC-MS) methods to determine the rate of antibiotic elution from orthopaedic bone cements, in collaboration with Novartis and The Northern General Hospital, Sheffield.</p><p>October 2011 - August 2012: Student Researcher - Paterson Institute for Cancer Research, Manchester</p><p>MRes project investigating the antisense regulation of cyclin D1 in breast cancer development and pathogenesis, utilising Western blotting, transfection, siRNA knockdown, gene</p><p>Page 1 of 4 overexpression, cell culture, immunohistochemistry and quantitative polymerase chain reactions (qPCR). </p><p>August 2010 - August 2011: Medical Laboratory Assistant - Royal Hallamshire Hospital, Sheffield</p><p>Working in the NHS cervical screening program preparation laboratory using the SurePath method of liquid-based cytology to prepare slides from patient samples for histological examination.</p><p>I have been continuously employed since the age of 16 in a variety of other roles, which include Housekeeper and Ward Assistant roles in the NHS, charity fundraising, waitressing, night club promotion and retail work, which aided me in developing a variety of skills including customer service and communication, along with team leader experience.</p><p>Publications:</p><p>Mason et al (2016) "Copper binding and subsequent aggregation of alpha-synuclein is modulated by N-terminal acetylation and is ablated by the H50Q missense mutation." Biochemistry - in press</p><p>Salih et al (2015) "The cement spacer with multiple indentations: increasing antibiotic elution using a cement spacer 'teabag'". Bone Joint J. 97-B(11):1519-24. doi: 10.1302/0301- 620X.97B11.35618</p><p>Conference Attendance and Disseminations:</p><p>13-15/09/2016: BMSS Annual Meeting (Eastbourne, UK) - Oral Presentation</p><p>17/12/2015: BMRC/MERI Christmas Poster Event (Sheffield, UK) - Poster Presentation</p><p>22/09/2015: Sheffield Glia Symposium (Sheffield, UK)</p><p>7-8/07/2015: BMSS IM-MS SIG Annual Meeting (Liverpool, UK) </p><p>27-30/06/2015: Brain & Brain PET (Vancouver, Canada) - Poster Presentation</p><p>11/12/2014: BMRC/MERI Christmas Poster Event (Sheffield, UK) - Poster Presentation</p><p>24-29/08/2014: IMSC (Geneva, Switzerland) - Poster Presentation 01-02/07/2014: BMSS IM-MS SIG Annual Meeting (Manchester, UK) </p><p>31/03/2014 - 02/04/2014: BMSS Annual Meeting (Manchester, UK) - Poster Presentation</p><p>17/12/2013: BMRC/MERI Christmas Poster Event (Sheffield, UK) - Poster Presentation</p><p>Public Engagement and Volunteering:</p><p>The Big Bang Yorkshire - June 2016 (Doncaster)</p><p>Page 2 of 4 Assisted the British Society for Immunology with their Britain Breathing stand.</p><p>230th Gleadless Scouts Science Fair - April 2016 (Sheffield) </p><p>Demonstrated the use of microscopy to observe a variety of materials.</p><p>Science and Engineering Week Event - March 2016 (Sheffield Hallam University) Ran a stall extracting DNA from strawberries with the public</p><p>Biotechnology YES Competition - October 2015 (GSK, Stevenage)</p><p>National competition entered with three fellow PhD students.</p><p>Work experience Supervision - July 2015 (Sheffield Hallam University)</p><p>Supervising local work experience students aged 15-18</p><p>3 Minute Thesis Competition - June 2015 (Sheffield Hallam University)</p><p>Successfully completed heat 1 and was a finalist for Sheffield Hallam University.</p><p>Science and Engineering Week Event - March 2015 (Sheffield Hallam University) </p><p>Work experience Supervision - July 2014 (Sheffield Hallam University)</p><p>Science and Engineering Week Event - March 2014 (Sheffield Hallam University) Work experience Supervision - July 2013 (Sheffield Hallam University)</p><p>Teaching Experience:</p><p>Throughout my PhD I have been involved in demonstrating and teaching in undergraduate and masters level laboratory classes, for both supervised and unsupervised periods. Classes assisted include forensics sessions determining caffeine concentration in hair samples, molecular biology classes identifying unknown protein concentrations, biochemistry labs investigating amino acids and titrations and the role of pH and buffers, and second year undergraduate project labs. I have been responsible for the majority of the training of the two PhD students succeeding me in my research group, and have trained and supervised MSci students. I have also been responsible for training Portuguese ERASMUS exchange students during their 6 month placements at the University. In addition, I have gained experience in teaching work experience students basic laboratory practices and techniques, and aided them in completing CREST award schemes during their time at the university.</p><p>Training Courses:</p><p>09/07/2016: Mass Spectrometry Imaging Symposium (Sheffield, UK)</p><p>18/04/2016: Royal Society of Biology Science Communication Workshop (London, UK)</p><p>28/04/2015 - 01/05/2015: Common Purpose Leadership Award (Sheffield, UK)</p><p>Page 3 of 4 01-02/07/2014: BMSS Ion Mobility-Mass Spectrometry Special Interest Group Training Course (Manchester, UK) </p><p>31/03/2014 - 02/04/2014: BMSS Introduction to Mass Spectrometry Course (Manchester, UK)</p><p>26/02/2016 - 27/04/2016 - Astrobiology and the Search for Extraterrestrial Intelligence - The UK Centre for Astrobiology, University of Edinburgh/Coursera (Online E-learning)</p><p>13/10/2015 - 30/11/2015 - Super-Earths and Life - HarvardX/EdX (Online E-learning)</p><p>Hobbies and Interests:</p><p>I have a passion for music and have been playing piano for many years. In addition, I have a keen interest in extreme sports and enjoy snowboarding and wakeboarding with friends and family as regularly as possible, and in as many different locations as I can. I love travelling to new places and hope to see most of the world in my time. I have also recently taken up amateur astronomy, and am slowly building a collection of my own photographs of both ours and Jupiter's moons.</p><p>References:</p><p>PhD Supervisor: Dr David Smith, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield, S1 1WB. [email protected]. 0114 225 3044</p><p>Research Centre Head: Prof Nicola Woodroofe, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield, S1 1WB. [email protected]. 0114 225 3065</p><p>Previous Employer: Prof Tom Smith, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield, S1 1WB. [email protected] . 0114 225 3042</p><p>Page 4 of 4</p>