<p>Virtual Lab: Testing Antibacterial Agents Name ______</p><p>Site: Classzone.com. Click on Biology Georgia 2008, then Virtual Labs, then Testing Antibacterial Agents</p><p>Do not print any of the pages from the computer. Place all of your answers on this sheet. You may have to fill in the log book but DO NOT print.</p><p>1. What is the purpose of this lab activity? ______</p><p>______</p><p>2. What is the purpose of the incubator? ______</p><p>______</p><p>3. Go to Background Information. Answer the following.</p><p> a. Bacteria that cause disease are called ______.</p><p> b. Most pathogens that enter the body are prevented from multiplying by the ______</p><p>______.</p><p> c. ______are substances that prevent bacterial growth.</p><p> d. ______are substances that are taken internally or </p><p> applied to the body to treat bacterial infections.</p><p> e. ______are bacterial agents that are safe enough to </p><p> apply to the skin, but not taken internally.</p><p> f. ______are substances used to clean surfaces but not</p><p> safe enough to apply to living tissues.</p><p>4. Predict whether each of the antibacterial agents will be E Effective, SE Somewhat Effective, or NE not effective in killing the bacteria.</p><p>Rate Test Substance Mouthwash Hydrogen Peroxide Bleach Household Disinfectant Alcohol Hand Soap</p><p>5. What is the control in the experiment? ______</p><p>6. After all of the disks have been soaked in the antibacterial agents and placed in the petri dishes, how long are they incubated? ______7. Why are the petri dishes stored upside down in the incubator? ______8. After the incubation period, you can click on the petri dishes to enlarge them. A clear area around the disk indicates no growth or that the bacterial agent on the disk is preventing the growth of bacteria. In the example below, A is effective, B is somewhat effective and C is not effective at all. ***Print the page to include with your answer sheet to be turned in.</p><p>In the chart below, indicate: NE NOT EFFECTIVE: normal growth (no clear areas around the disk) SE SOMEWHAT EFFECTIVE: less growth ( a narrow margin around the disk) E EFFECTIVE: no growth (a ring of clear area around the disk) </p><p>Rate Test Substance Mouthwash Hydrogen Peroxide Bleach Household Disinfectant Alcohol Hand Soap</p><p>9. According to your results, what was the best antibacterial agent to use against the bacteria? ______</p><p>10. Which ones were somewhat effective? ______</p><p>11. Which ones were not effective at all? ______</p><p>12. Did the results match your predictions? Explain. ______</p><p>13. Which of the agents that you tried are: (a) antiseptics ______</p><p>(b) antibiotics ______</p><p>(c) disinfectants ______</p><p>14. Based on your observations, can you accurately predict how effective the antibacterial agents used in this investigation will be against different types of bacteria? Explain. ______15. In this experiment, identify the (a) independent variable ______</p><p>(b) dependent variable ______</p>