<p> 1 FUNDED BY</p><p>THE BRAIN TUMOUR TISSUE BANK</p><p>Application for Tissue</p><p>The Brain Tumour Tissue Bank provides scientists with optimally collected, dissected, and stored human brain tumor tissues. Brain tumour specimens, body fluids, and paraffin sections from a wide variety of pathologically verified tumors are available.</p><p>The Brain Tumour Tissue Bank collects appropriate specimens at the time of operation, immediately freezing and storing the specimens in liquid nitrogen (at -196C) . These tissues are made available to qualified scientists working in a number of research disciplines in the hopes that some day the cause of and cure for brain tumours will be found.</p><p>Types of Tissues Available</p><p>In addition to brain tumour tissues (snap frozen, snap frozen in OCT and paraffin sections), associated body fluids are available in some cases, including:</p><p>• Whole blood (in ACD anticoagulant)</p><p>• ** Serum • ** Cerebral spinal fluid • ** Tumour cyst fluid</p><p>** Please note, these samples are no longer collected and would be available only with some older cases. 2</p><p>Quality Control</p><p>The in house tissue grading system indicated below, was developed by the Brain Tumour Tissue Bank and is recorded by observing the H&E stained quality control slides. Technically, the tissue grade of the QC section should be very similar to the frozen tissue since it is the immediate adjacent representative piece of the frozen tissue. All tumours are graded as G (good), M (moderate), or P(poor) specimens, depending on the amount of tumour cells present (as opposed to hemorrhagic, necrotic, and fibrous tissues). In addition, all infiltrating tumours (gliomas) are further categorized as follows: </p><p>Note: If requesting “glioma samples”, please indicate on application which tissue grades are best suitable for your study. It is common to get a mixture of these in the same sample.</p><p>TISSUE GRADE FOR GLIOMAS</p><p> i tumour centre ii heavy tumour infiltrate iii light tumour infiltrate iv scant tumour cells (peritumour) v no visible tumour cells</p><p>Priorities</p><p>Studies focused on the mechanism of cerebral tumour generation, growth, and treatment. </p><p>Please e-mail your application to [email protected]</p><p>3</p><p>1. Applicants</p><p>1.1 Name: </p><p>Position:</p><p>Academic Affiliation:</p><p>Address:</p><p>Telephone:</p><p>Fax:</p><p>E-mail:</p><p>1.2 Name:</p><p>Position:</p><p>Academic Affiliation:</p><p>Address:</p><p>Telephone:</p><p>Fax:</p><p>E-mail: 4</p><p>2. Title of Research ______</p><p>3. Key Words</p><p>List eight words, which best describe the research:</p><p>4. Funding 5 5. Summary of Research ______</p><p>Please provide a brief summary of your project. This summary may be used for publications of the Brain Tumour Tissue Bank as well as its funding agency the Brain Tumour Foundation of Canada. If applicable, please describe the implications of your research project for brain tumour patients - how this research may affect treatments and therapy in the future. 6 6. Tissues Requested ______</p><p>Please be as specific as possible.</p><p>6.1Tumour type, number requested and tissue grade preferred (chart on page 2) Please indicate if recurrent samples are acceptable:</p><p>6.2 Type of specimen requested:</p><p>Snap frozen ______</p><p>* Snap frozen in OCT (cryostat ready) ______</p><p>Paraffin sections (Number of slides) ______</p><p>6.3 Request for other tissues and fluids (indicate volume if fluid requested):</p><p>* Whole blood ______</p><p>* Serum ______</p><p>* CSF ______</p><p>* Cyst fluid ______</p><p>* Please note that not all are available with each tumour case.</p>