<p>Supplement Fig. 1 The amplification plot of caspase-3 mRNA by RT-PCR The</p><p>SW620 cells were pretreated with curcumin (10 μM) for 1 h followed by treatment with PAR2-AP (100 μM) or FVIIa (10 nM) for additional 4 h. The mRNA levels of caspase-3 andβ-actin were detected by real-time quantitative RT-PCR A. the amplification of β-actin mRNA B. the standards curve of β-actin mRNA C. the amplification of caspase-3 mRNA D. the standards curve of caspase-3 mRNA Supplement Fig. 2 The amplification plot of TF mRNA RT-PCR The SW620 cells were pretreated with curcumin (10 μM) for 1 h followed by treatment with</p><p>PAR2-AP (100 μM) or FVIIa (10 nM) for additional 24 h. The mRNA levels of TF andβ-actin were detected by real-time quantitative RT-PCR A. the amplification of β- actin mRNA B. the standards curve of β-actin mRNA C. the amplification of TF mRNA D. the standards curve of TF mRNA Supplement Fig. 3 The amplification plot of MMP-9 mRNA RT-PCR The</p><p>SW620 cells were pretreated with curcumin (10 μM) for 1 h followed by treatment with PAR2-AP (100 μM) or FVIIa (10 nM) for additional 12 h. The mRNA levels of</p><p>MMP-9 andβ-actin were detected by real-time quantitative RT-PCR A. the amplification of β-actin mRNA B. the standards curve of β-actin mRNA C. the amplification of MMP-9 mRNA D. the standards curve of MMP-9 mRNA</p>