<p>Journal of Consumer Protection and Food Safety</p><p>A new 7-plex PCR assay for simultaneous detection of shiga toxin-producing</p><p>Escherichia coli O157 and Salmonella Enteritidis in meat products</p><p>Yuexia Wanga1, Biao Suoa2*</p><p>1 College of Life Sciences, Henan Agricultural University, 63 Nongye Rd. Zhengzhou,</p><p>Henan Province 450002, China</p><p>2 College of Food Science & Technology, Henan Agricultural University, 63 Nongye</p><p>Rd. Zhengzhou, Henan Province 450002, China</p><p>*Corresponding author:</p><p>Biao Suo</p><p>E-mail: [email protected] a Yuexia Wang and Biao Suo contributed equally to this work.</p><p>1 Supplemental Table 1. Bacterial strains used in the specificity test of multiplex PCR </p><p>Strains Multiplex PCR Results</p><p> invA SdfI rfbE eae stx1 stx2 IAC S. enterica Enteritidis CMCC 50335 + + - - - - - S. enterica Enteritidis ATCC 13076 + + - - - - - S. enterica Typhimurium ATCC 13311 + ------S. enterica Typhimurium ATCC 14028 + ------S. enterica Infantis ATCC 51741 + ------S. enterica Anatum ATCC 9270 + ------S. enterica Montevideo ATCC 8387 + ------S. enterica Gallinarum CMCC 50770 + ------S. enterica Saintpaul ATCC 9712 + ------S. enterica Vellore ATCC 15611 + ------S. enterica Tallahassee ATCC 12002 + ------E. coli O157:H7 ATCC 43895 - - + + + + - E. coli O157:H7 ATCC 43890 - - + + + - - E. coli O157:H7 ATCC 43889 - - + + - + - E. coli O157:H7 ATCC 43888 - - + + - - - L. monocytogenes ATCC54002 ------Enterobacter sakazakii ATCC29544 ------Staphylococcus aureus ATCC 2593 ------Enterococcus faecalis ATCC 49452 ------Enterobacter sakazakii ATCC 29544 ------Proteus mirabilis ATCC 12453 ------Citrobacter freundii ATCC 8090 ------Aeromonas hydrophila ATCC 7965 ------Enterococcus avium ATCC 14025 ------Staphylococcus epidermidis ATCC 11993 ------Staphylococcus simulaus ATCC 13044 ------Micrococcus luteus ATCC 9341 ------(+) Positive results; (-) Negative results.</p><p>2 Supplemental Table 2. Comparison results of traditional microbiological method and multiplex PCR in detection of meat samples.</p><p>Food name Detection method</p><p>Multiplex PCR Microbiological analysis</p><p>E. coli O157 Salmonella IAC E. coli O157 Salmonella (Enteritidis) rfbE eae stx1 stx2 invA sdfI</p><p>Raw sausage 1 (A) + + + + + + - + + (+) Raw sausage 2 (N) ------+ - - (-) Raw sausage 3 (N) - - - - + + + - + (+) Raw sausage 4 (N) ------+ - - (-) Raw sausage 5 (C) ------+ - - (-) Cooked ham 1 (A) + + + + + + - + + (+) Cooked ham 2 (N) ------+ - - (-) Cooked ham 3 (N) ------+ - - (-) Cooked ham 4 (N) ------+ - - (-) Cooked ham 5 (C) ------+ - - (-) Raw Duck 1 (A) + + + + + + - + + (+) Raw Duck 2 (N) ------+ - - (-) Raw Duck 3 (N) ------+ - - (-) Raw Duck 4 (N) + + - - - - + + - (-) Raw Duck 5 (C) ------+ - - (-) Raw Beef 1 (A) + + + + + + - + + (+) Raw Beef 2 (N) ------+ - - (-) Raw Beef 3 (N) + + - - + + + - + (+) Raw Beef 4 (N) ------+ - - (-) Raw Beef 5 (C) ------+ - - (-) Raw Chicken 1 (A) + + + + + + - + + (+) Raw Chicken 2 (N) ------+ - - (-) Raw Chicken 3 (N) - - - - + + + - + (+) Raw Chicken 4 (N) - - - - + - + - + (-) Raw Chicken 5 (C) ------+ - - (-) Raw Lamb 1 (A) + + + + + + - + + (+) Raw Lamb 2 (N) - - - - + + + - + (+) Raw Lamb 3 (N) ------+ - - (-) Raw Lamb 4 (N) ------+ - - (-) Raw Lamb 5 (C) ------+ - - (-) Raw pork 1 (A) + + + + + + - + + (+) Raw pork 2 (N) ------+ - - (-) Raw pork 3 (N) + + - + + + - + + (+) Raw pork 4 (N) - - - - + - + - + (-)</p><p>3 Raw pork 5 (C) ------+ - - (-) (N) naturally contaminated food; (A) artificially contaminated food; (C) negative control; (+) positive results; (-) negative results.</p><p>4</p>