<p> Brain morphometry and FMS symptoms SUPPLEMENTARY MATERIAL</p><p>SUPPLEMENTARY MATERIAL</p><p>THE IMPACT OF FIBROMYALGIA SYMPTOMS ON BRAIN MORPHOMETRY</p><p>Carolina Diaz-Piedraa,b, Manuel A. Guzmanc, Gualberto Buela-Casala, & Andres Catenaa Brain Imaging and Behavior Corresponding author: [email protected]</p><p> aMind, Brain, and Behavior Research Center-CIMCYC; University of Granada; Campus de Cartuja s/n; 18071 Granada; Spain bCollege of Nursing & Health Innovation; Arizona State University; 550 N. 3rd St.; 85004 Phoenix, AZ, USA cService of Rheumatology; University Hospital Virgen de las Nieves; Avda. de las Fuerzas Armadas 2; 18014 Granada; Spain</p><p>1. CLINICAL DATA</p><p>Supplemental Table 1 Descriptive statistics for pain intensity, psychological distress, anxiety, depression, sleep- related, and health-status measures of patients with fibromyalgia syndrome and healthy controls.</p><p>Fibromyalgia patients Healthy controls N=23 N=23 Mean (SD) Mean (SD) Pain intensity* 6.1 (1.9) 0.9 (1.3) Psychological distress: HADS* 20.3 (7.2) 9.9 (6.1) Anxiety: STAI-T* 34.7 (11.4) 18.4 (10.9) Anxiety: STAI-S* 24.9 (11.4) 13.7 (8.8) Depression: BDI* 24.7 (12.2) 6.4 (7.2) Sleep quality: PSQI* 14.2 (3.9) 3.6 (2.3) Diurnal sleepiness: ESS* 10.9 (5.9) 5.0 (3.8) Health status: FIQ* 63.5 (15.3) 6.9 (7.4) Note. SD = Standard deviation; HADS = Hospital Anxiety and Depression Scale; STAI = State-Trait Anxiety Inventory; PSQI = Pittsburgh Sleep Quality Index; ESS = Epworth Sleepiness Scale; FIQ = Fibromyalgia Impact Questionnaire. * p<.05 Brain morphometry and FMS symptoms SUPPLEMENTARY MATERIAL</p><p>2. COMPARISON OF LOCAL BRAIN VOLUMES BETWEEN FMS PATIENTS AND HEALTHY CONTROLS</p><p>FMS patients exhibited less gray matter volume (GMV) in the left medial and superior orbital frontal gyrus (BA11) and greater GMV in the temporal pole (BA38) (corrected p-values<.05). No differences in local WMV were observed between groups (all corrected p-values>.05). </p><p>Supplemental Table 2 Local gray matter volume differences in patients with fibromyalgia syndrome (N=23) relative to healthy controls (N=23).</p><p>Effect Lobe Area BA k T x y z FMS < Controls Frontal L Frontal Med/Sup Orb 11 469 5.22 -17 53 -24 FMS > Controls Frontal L Temporal Pole 38 542 4.49 -53 15 -29 Note. FMS=Fibromyalgia syndrome; L=Left; Med=Medial; Sup=Superior; Orb=Orbital; BA=Brodmann area; k=number of voxels; T=Peak t values; x, y, z= Montreal Neurological Institute coordinates (in mm).</p><p>Supplemental Fig 1 Coronal, sagittal, and axial brain slices showing the areas exhibiting significant gray matter volume (GMV) differences between fibromyalgia syndrome (FMS) patients (N=23) and healthy controls (N=23) (corrected p<.05), superimposed on a high-resolution T1-weighted template (generated by averaging 46 scans). Brain morphometry and FMS symptoms SUPPLEMENTARY MATERIAL</p><p>Areas with reduced GMV in FMS vs. healthy controls are shown in blue; areas with increased GMV in FMS vs. healthy controls are shown in red. Coordinates are shown in Supplemental Table 2.</p>