<p> CURRICULUM VITAE November, 2016</p><p>ERIC A. NELSON Veterinary & Biomedical Sciences/ADRDL South Dakota State University Brookings, South Dakota 57007 Phone: 605-688-5653 E-mail: [email protected] ______Education Ph.D., 1993, Biological Sciences/Veterinary Microbiology, South Dakota State University, Brookings, SD. M.A., 1981, Zoology. University of South Dakota, Vermillion, SD. B.A., 1979, Chemistry/Biology, Mount Marty College, Yankton, SD. </p><p>PhD Dissertation Title Nelson, E. (1993). Characterization of Porcine Reproductive and Respiratory Syndrome (PRRS) Virus. South Dakota State University, Brookings, SD, p. 1-131.</p><p>Appointments/Professional Experience Assistant Department Head: Veterinary & Biomedical Sciences/ADRDL, 2013-present South Dakota State University, Brookings, SD. Adjunct Professor: School of Veterinary Medicine and Biomedical Sciences, 2010-present University of Nebraska-Lincoln, NE. Professor: Veterinary & Biomedical Sciences/ADRDL, 2003-present South Dakota State University, Brookings, SD. Associate Professor: Department of Veterinary Science/ADRDL, 1998-2003 South Dakota State University, Brookings, SD. Assistant Professor: Department of Veterinary Science/ADRDL 1994-1998 South Dakota State University, Brookings, SD. Instructor: Department of Veterinary Science/ADRDL, 1988-1994 South Dakota State University, Brookings, SD. Research Scientist: NOBL Laboratories, Inc., Sioux Center, IA. 1987-1988 Assistant in Virology: Department of Veterinary Science, South Dakota State University, Brookings, SD. 1982-1987</p><p>Selected Awards Joseph J. Garbarino Achievement Award. Presented by the Animal Health Institute Foundation for original research in animal health, 1994.</p><p>South Dakota State University Gamma Sigma Delta Research Award, 2009.</p><p>SDSU Technology Transfer Office Intellectual Property Commercialization Award, 2009.</p><p>Pat and Jo Scott Cannon Intellectual Property Commercialization Award, 2015.</p><p>Professional Service and Societies American Society for Virology Conference of Research Workers in Animal Disease American Association of Veterinary Laboratory Diagnosticians American Association of Swine Veterinarians</p><p>1 Sigma Xi South Dakota ADRDL Serology Section Leader</p><p>Selected Recent Refereed Publications Joshi, L.R., M.H.V. Fernandes, T. Clement, S. Lawson, A. Pillatzki, T.P Resende, F. Vanucci, G.F. Kutish, E.A. Nelson, D.G. Diel. 2016. Pathogenesis of Senecavirus A infection in finishing pigs. J. Gen. Virol. doi: 10.1099/jgv.0.000631.</p><p>Hain, K.S., L.R. Joshi, F. Okda, J. Nelson, A. Singrey, S. Lawson, A. Pillatzki, G.F. Kutish, E.A. Nelson, E.F. Flores, D.G. Diel. 2016. Immunogenicity of a recombinant parapoxvirus expressing the spike protein of porcine epidemic diarrhea virus. J. Gen. Virol. 97:2719-2731. doi: 10.1099/jgv.0.000586.</p><p>Vitosh-Sillman, S., J. Loy, B. Brodersen, C. Kelling, A. Doster, C. Topliff, E. Nelson, J. Bai, L. Peddireddi, B. Hause, R. Hesse. 2016. Experimental infection of conventional nursing pigs and their dams with porcine deltacoronavirus. J. Vet. Diagn. Invest. 28:468-497.</p><p>Diel, D.G., S. Lawson, F. Okda, A. Singrey, T. Clement, M.H.V. Fernandes, J. Christopher-Hennings, E.A. Nelson. 2016. Porcine epidemic diarrhea virus: an overview of current virological and serological diagnostic methods. Virus Res. doi.org/10.1016/j.virusres. 2016.05.013.</p><p>Song, Y., P. Singh, E. Nelson, S. Ramamoorthy. 2016. A computationally-designed serological assay for the porcine epidemic diarrhea virus. J. Clin. Micro. doi:10.1128/JCM.00460-16.</p><p>Okda, F., S. Lawson, X. Liu, A. Singrey, T. Clement, J. Nelson, J. Christopher-Hennings, E.A. Nelson. 2016. Development of monoclonal antibodies and serological assays including indirect ELISA and fluorescent microsphere immunoassays for diagnosis of porcine deltacoronavirus. BMC Vet. Res. 12:95. doi: 10.1186/s12917-016-0716-6.</p><p>Clement, T., A. Singrey, S. Lawson, F. Okda, J. Nelson, D. Diel, E.A. Nelson, J. Christopher-Hennings. 2016. Quantitation of porcine epidemic diarrhea virus neutralizing antibodies in sow serum, colostrum, milk and piglet serum after feedback. J. Swine Health and Prod. 24:147-153.</p><p>Useh, N.M., E.O. Ngbede, N. Akange, M. Thomas, A. Foley, M. Chan Keena, E. Nelson, J. Christopher- Hennings, M. Tomita, H. Suzuki, J. Scaria. 2016. Draft genome sequences of 37 Salmonella enterica strains isolated from poultry sources in Nigeria. Genome Announc. 4(3):e00315-16. doi:10.1128/genomeA.00315-16.</p><p>Joshi, L., K. Mohr, T. Clement, K. Hain, B. Myers, J. Yaros, E. Nelson, J. Christopher-Hennings, D. Gava, R. Schaefer, L. Caron, S. Dee, D. Diel. 2016. Detection of the newly emerging Senecavirus A in pigs, mice and houseflies. J. Clin. Micro. 54:1536-1545. doi: 10.1128/JCM.03390-15.</p><p>Wang, X., H. Zhang, A.M. Abel, E. Nelson. 2016. Protein kinase R (PKR) plays a pro-viral role in porcine reproductive and respiratory syndrome virus (PRRSV) replication by modulating viral gene transcription. Arch. of Virol. 161:327-333. 10.1007/s00705-015-2671-0.</p><p>Swartz, J.E., D.W. Brake, E.E. Grings, E.A. Nelson, C.L. Wright, J.A. Walker, E.J. Blon, G.A. Perry. 2016. Effects of enzymatically hydrolyzed yeast supplementation and supplementation frequency on nitrogen balance and apparent digestibility in periparturient beef cows. J. Animal Science 94(2)169-170.</p><p>2 Dee, S., C. Neill, A. Singrey, T. Clement, R. Cochrane, C. Jones, G. Patterson, J. Christopher-Hennings, E. Nelson. 2016. Modeling the transboundary risk of feed ingredients contaminated with porcine epidemic diarrhea virus. BMC Vet. Res. 12:51.</p><p>Sreenivasan, C., M. Thomas, Z. Sheng, B. Hause, E. Collin, D. Knudsen, A. Pillatzki, E. Nelson, D. Wang, R. Kaushik, F. Li. 2015. Replication and transmission of novel bovine influenza D virus in guinea pig model. J. Virol. 89:11990-12001.</p><p>Quast, M., C. Sreenivasan, G. Sexton, A. Singrey, L. Fawcett, D. Lauer, S. Voss, S. Pollock, C.W. Cunha, J. Christopher-Hennings, E. Nelson, F. Li. 2015. Serological evidence for the presence of influenza D virus in small ruminants. Vet. Micro. 180(3-4):281-285.</p><p>Ran, Z., H. Shen, Y. Lang, E. Kolb, N. Turan, L. Zhu, J. Ma, B. Bawa, Q. Liu, H. Liu, M. Quast, G. Sexton, F. Krammer, B. Hause, J. Christopher-Hennings, E. Nelson, J. Richt, F. Li, and W. Ma. 2015. Domestic pigs are susceptible to infection with influenza B viruses. J. Virol. 89:4818-4826.</p><p>Collin, E.A., S. Anbalagan, F. Okda, R. Batman, E.A. Nelson, B.M. Hause. 2015. An inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs. BMC Vet. Res. 11:62.</p><p>Robinson, S., J. Li, E.A. Nelson, M.P. Murtaugh. 2015. Broadly neutralizing antibodies against the rapidly evolving Porcine reproductive and respiratory syndrome virus. Virus Res. 203:56-65.</p><p>Clement, T., G. Kutish, J. Nezworski, J. Scaria, E. Nelson, J. Christopher-Hennings, D. Diel. 2015. Complete genome sequence of a highly pathogenic avian influenza virus (H5N2) associated with an outbreak in commercial chickens, Iowa, USA, 2015. Genome Announc. 3(3):e00613-15. doi:10.1128/genomeA.00613-15. </p><p>Okda, F., X. Liu, A. Singrey, T. Clement, J. Nelson, J. Christopher-Hennings, E.A. Nelson, S. Lawson. 2015. Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus. BMC Vet. Res. 11:180. doi:10.1186/s12917-015-0500-z.</p><p>Dee, S., C. Neill, T. Clement, A. Singrey, J. Christopher-Hennings, E. Nelson. 2015. An evaluation of porcine epidemic diarrhea virus survival in individual feed ingredients in the presence or absence of a liquid antimicrobial. Porcine Health Management. 1:9 doi:10.1186/s40813-015-0003-0.</p><p>Hause, B., E. Collin, R. Liu, B. Huang, Z. Sheng, W. Lu, D. Wang, E. Nelson, F. Li. 2014. Characterization of a novel influenza virus in cattle and swine: Proposal for a new genus in the Orthomyxoviridae family. mBio 5(2):e00031-14. doi:10.1128.</p><p>Dee S., C. Neill, T. Clement, J. Christopher-Hennings, E. Nelson. 2014. An evaluation of a liquid antimicrobial (Sal CURB®) for reducing the risk of porcine epidemic diarrhea virus infection of naïve pigs during consumption of contaminated feed. BMC Vet. Res. 10:220. </p><p>3 Dee S., T. Clement, A. Schelkopf, J. Nerem, D. Knudsen, J. Christopher-Hennings, E. Nelson. 2014. An evaluation of contaminated complete feed as a vehicle for porcine epidemic diarrhea virus infection of naïve pigs following consumption via natural feeding behavior: Proof of concept. BMC Vet. Res. 10:176. </p><p>Christopher-Hennings J., K. Araujo, Y. Fang, S. Lawson, E. Nelson, J. Lunney. 2013. Considerations for performing Luminex multianalyte profiling (xMAP) in veterinary diagnostic laboratories. J. Vet. Diagn. Invest. 25:773.</p><p>Christopher-Hennings J, K.P.C. Araujo, C.J.H. Souza, Y. Fang, S. Lawson, E.A. Nelson, T. Clement, M. Dunn, J.K. Lunney. 2013. Opportunities for bead-based multi-plex assays in veterinary diagnostic laboratories. J. Vet. Diagn. Invest. 25:671-691.</p><p>Olsen C., C. Wang, J. Christopher-Hennings, K. Doolittle, K. Harmon, S. Abate, A. Kittawornrat, S. Lizano, R. Main, E. Nelson, T. Otterson, Y. Panyasing, C. Rademacher, R. Rauh, R. Shah, J. Zimmerman. 2013. Probability of detecting porcine reproductive and respiratory syndrome virus infection using pen-based swine oral fluid specimens as a function of within-pen prevalence. J. Vet. Diagn. Invest. 25:328-325. </p><p>Zhang, H., X. Guo, E. Nelson, J. Christopher-Hennings, X. Wang. 2012. Porcine reproductive and respiratory syndrome virus activates the transcription of interferon alpha/beta (IFN-α/β) in monocyte- derived dendritic cells (Mo-DC). Vet. Microbiol. 159:494.</p><p>Anderson, T., W. Laegreid, F. Cerutti, F. Osorio, E. Nelson, J. Christopher-Hennings, T. Goldberg. 2012. Ranking viruses: Measures of positional importance within networks define core viruses for rational polyvalent vaccine development. Bioinformatics 28:1624-1632. </p><p>Lawson, S., Y. Li, J. Patton, R.J. Langenhorst, Z. Sun, Z. Jiang, J. Christopher-Hennings, E.A. Nelson, D. Knudsen, Y. Fang, K.-O. Chang. 2012. Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus. Virus Res. 163:461-468.</p><p>Langohr, I.M., G.W. Stevenson, E.A. Nelson, S.D. Lenz, H., R.M. Pogranichniy. 2012. Experimental co- infection of pigs with bovine viral diarrhea virus 1 and porcine circovirus-2. J. Vet. Diagn. Invest. 24:51- 64.</p><p>Kittawornrat, A., C. Wang, G. Anderson, A. Ballagi, A. Broes, S. Carman, K. Doolittle, J. Galeota, J. Johnson, S. Lizano, E. Nelson, D. Patnayak, R. Pogranichniy, A. Rice, G. Scherba, J. Zimmerman. 2012. Ring test evaluation for the detection of PRRSV antibodies in oral fluid specimens using a commercial PRRSV serum antibody ELISA. J. Vet. Diagn. Invest. 24:1057-1063.</p><p>Langenhorst, R., S. Lawson, A. Kittawornrat, J. Zimmerman, Z. Sun, Y. Li, J. Christopher-Hennings, E. Nelson, Y. Fang. 2012. Development of a fluorescent microsphere immunoassay for detection of antibodies against PRRSV using oral fluid samples as an alternative to serum-based assays. Clin. and Vacc. Immunol. 19:180-189.</p><p>Brockmeier, S.L., C.L. Loving, E.A. Nelson, L.C. Miller, T.L. Nicholson, K.M. Register, M.J. Grubman, D.E. Brough, M.E. Kehrli. 2012. Effects of interferon-α on the immune response to porcine reproductive and respiratory syndrome virus. Clin. and Vacc. Immunol. 19:508-514. </p><p>4 Lawson, S., J. Lunney, F. Zuckermann, F. Osorio, E. Nelson, C. Welbon, T. Clement, Y. Fang, S. Wong, K. Kulas, J. Christopher-Hennings. 2010. Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: Assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination. Vaccine 28:5383-5391.</p><p>Wang, D., A. Harmon, J. Jin, D.H. Francis, J. Christopher-Hennings, E. Nelson, R.C. Montelaro, F. Li. 2010. The lack of an inherent membrane targeting signal is responsible for the failure of the matrix (M1) protein of influenza A virus to bud into virus-like particles. J. Virol. 84:4673-4681.</p><p>Chen, Z., X. Zhou, J.K. Lunney, S. Lawson, Z. Sun, E. Brown, J. Christopher-Hennings, D. Knudsen, E.A. Nelson, Y. Fang. 2010. Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication but play an important role in modulation of host immune response. J. Gen. Virol. 91:1047-1057.</p><p>Chen, Z., S. Lawson, Z. Sun, X. Zhou, X. Guan, J. Christopher-Hennings, E.A. Nelson, Y. Fang. 2010. Identification of two autocleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virol. 398:87-97.</p><p>Langohr, I.M., G.W. Stevenson, E.A. Nelson, S.D. Lenz, H. HogenEsch, H. Wei, R.M. Pogranichniy. 2010. Vascular lesions in pigs experimentally infected with porcine circovirus type 2b. Vet. Pathol. 47:140-147.</p><p>Kinch, M.S., A.S. Yunus, C. Lear, H. Mao, H. Chen, Z. Fesseha, G. Luo, E.A. Nelson, L. Li, L. Huang, M. Mury, W.Y. Ellis, L. Hensley, J. Christopher-Hennings, G.G. Olinger, M. Goldblatt. 2009. FGI-104: a broad-spectrum small molecule inhibitor of viral infection. Am. J. Trans. Res. 1:87-98.</p><p>Brown, E., S. Lawson, C. Welbon, J. Gnanandarajah, J. Li, M.P. Murtaugh, E.A. Nelson, R.M. Molina, J.J. Zimmerman, R.R.R. Rowland, Y. Fang. 2009. Antibody response of nonstructural proteins: Implication for diagnostic detection and differentiation of Type I and Type II porcine reproductive and respiratory syndrome viruses. Clin. and Vacc. Immunol. 16:628-635.</p><p>Spilman, M.S., C. Welbon, E.A. Nelson, T. Dokland. 2009. Cryo-electron tomography of porcine reproductive and respiratory syndrome virus (PRRSV): Organization of the nucleocapsid. J. Gen. Virol. 90:527-535.</p><p>Molina, R.M., E.A. Nelson, J. Christopher-Hennings, R. Hesse, R.R. Rowland, J.J. Zimmerman. 2009. Evaluation of the risk of PRRSV transmission via ingestion of muscle from persistently-infected pigs. Transboundary and Emerging Diseases 56:1-8.</p><p>Fang, Y., J. Christopher-Hennings, E. Brown, H. Liu, Z. Chen, S. Lawson, R. Breen, T. Clement, X. Gao, J. Bao, D. Knudsen, R. Daly, E.A. Nelson. 2008. Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: Implications for future recombinant marker vaccine development. J. Gen. Virol. 89:3086-3096.</p><p>Molina, R.M., W. Chittick, E.A. Nelson, J. Christopher-Hennings, R.R.R. Rowland, J.J. Zimmerman. 2008. Diagnostic performance of assays for the detection of anti-PRRSV antibodies in porcine muscle transudate “meat juice” samples. J. Vet. Diagn. Invest. 20:735-743.</p><p>5 Molina, R.M., S.-H. Cha, W. Chittick, S. Lawson, M.P. Murtaugh, E.A. Nelson, J. Christopher-Hennings, K.-J. Yoon, R. Evans, R.R.R. Rowland, J.J. Zimmerman. 2008. Immune response against PRRSV during acute and chronic infection. Vet. Immunol. Immunopath. 126:283-292. </p><p>Cafruny, W.A., R.G. Duman, R.R. Rowland, E.A. Nelson, G.H. Wong. 2008. Antibiotic-mediated inhibition of porcine reproductive and respiratory syndrome virus (PRRSV) infection: A novel quinolone function which potentiates the antiviral cytokine response in MARC-145 cells and pig macrophages. Virology: Research and Treatment 1:1-12.</p><p>Technology Transfer/Commercialization </p><p>17 SDSU invention disclosures </p><p>15 Commercially licensed technologies</p><p>17 Patent applications based on disclosed technologies</p><p>Patents Awarded</p><p>Recombinant North American type 1 porcine reproductive and respiratory syndrome virus and methods of use. Fang, Y., E.A. Nelson and J. Christopher-Hennings. U.S. patent # 8182984. Issued May 22, 2012.</p><p>6</p>